دورية أكاديمية
Barrier-Forming Potential of Epithelial Cells from the Exstrophic Bladder.
| العنوان: | Barrier-Forming Potential of Epithelial Cells from the Exstrophic Bladder. |
|---|---|
| المؤلفون: | Hinley J; Jack Birch Unit for Molecular Carcinogenesis, Department of Biology and York Biomedical Research Institute, University of York, York, United Kingdom., Duke R; Jack Birch Unit for Molecular Carcinogenesis, Department of Biology and York Biomedical Research Institute, University of York, York, United Kingdom., Jinks J; Jack Birch Unit for Molecular Carcinogenesis, Department of Biology and York Biomedical Research Institute, University of York, York, United Kingdom., Stahlschmidt J; Jack Birch Unit for Molecular Carcinogenesis, Department of Biology and York Biomedical Research Institute, University of York, York, United Kingdom; Department of Histopathology, St James's University Hospital, Leeds, United Kingdom., Keene D; Department of Paediatric Urology, Royal Manchester Children's Hospital, Manchester, United Kingdom., Cervellione RM; Department of Paediatric Urology, Royal Manchester Children's Hospital, Manchester, United Kingdom., Mushtaq I; Department of Paediatric Urology, Great Ormond Street Hospital for Children National Health Service Trust, London, United Kingdom., De Coppi P; Department of Paediatric Urology, Great Ormond Street Hospital for Children National Health Service Trust, London, United Kingdom; National Institute for Health and Care Research Biomedical Research Centre, University College London Great Ormond Street Institute of Child Health, London, United Kingdom. Electronic address: p.decoppi@ucl.ac.uk., Garriboli M; National Institute for Health and Care Research Biomedical Research Centre, University College London Great Ormond Street Institute of Child Health, London, United Kingdom; Paediatric Urology, Evelina London Children's Hospital, London, United Kingdom., Southgate J; Jack Birch Unit for Molecular Carcinogenesis, Department of Biology and York Biomedical Research Institute, University of York, York, United Kingdom. Electronic address: j.southgate@york.ac.uk. |
| المصدر: | The American journal of pathology [Am J Pathol] 2022 Jun; Vol. 192 (6), pp. 943-955. Date of Electronic Publication: 2022 Mar 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0370502 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-2191 (Electronic) Linking ISSN: 00029440 NLM ISO Abbreviation: Am J Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011-: New York : Elsevier Original Publication: Philadelphia [etc.] American Assn. of Pathologists [etc.] |
| مواضيع طبية MeSH: | Carcinoma, Squamous Cell*/pathology , Urinary Bladder*/metabolism, Cell Differentiation ; Epithelial Cells/metabolism ; Humans ; Urothelium/metabolism |
| مستخلص: | Bladder exstrophy (BEX) is a rare developmental abnormality resulting in an open, exposed bladder plate. Although normal bladder urothelium is a mitotically quiescent barrier epithelium, histologic studies of BEX epithelia report squamous and proliferative changes that can persist beyond surgical closure. The current study examined whether patient-derived BEX epithelial cells in vitro were capable of generating a barrier-forming epithelium under permissive conditions. Epithelial cells isolated from 11 BEX samples, classified histologically as transitional (n = 6) or squamous (n = 5), were propagated in vitro. In conditions conducive to differentiated tight barrier formation by normal human urothelial cell cultures, 8 of 11 BEX lines developed transepithelial electrical resistances of more than 1000 Ω.cm 2 , with 3 squamous lines failing to generate tight barriers. An inverse relationship was found between expression of squamous KRT14 transcript and barrier development. Transcriptional drivers of urothelial differentiation PPARG, GATA3, and FOXA1 showed reduced expression in squamous BEX cultures. These findings implicate developmental interruption of urothelial transcriptional programming in the spectrum of transitional to squamous epithelial phenotypes found in BEX. Assessment of BEX epithelial phenotype may inform management and treatment strategies, for which distinction between reversible versus intractably squamous epithelium could identify patients at risk of medical complications or those who are most appropriate for reconstructive tissue engineering strategies. (Copyright © 2022 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | MR/N028414/1 United Kingdom MRC_ Medical Research Council; MR/L013347/1 United Kingdom MRC_ Medical Research Council; United Kingdom DH_ Department of Health |
| تواريخ الأحداث: | Date Created: 20220331 Date Completed: 20220613 Latest Revision: 20220716 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9194657 |
| DOI: | 10.1016/j.ajpath.2022.03.009 |
| PMID: | 35358476 |
| قاعدة البيانات: | MEDLINE |
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