دورية أكاديمية
Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model.
| العنوان: | Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model. |
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| المؤلفون: | Muñiz XF; Instituto de Agrobiotecnología y Biotecnología Molecular (IABIMO), UEDD INTA-CONICET; CICVyA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina; CONICET, Argentina., García EA; Instituto de Agrobiotecnología y Biotecnología Molecular (IABIMO), UEDD INTA-CONICET; CICVyA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina; CONICET, Argentina., Blanco FC; Instituto de Agrobiotecnología y Biotecnología Molecular (IABIMO), UEDD INTA-CONICET; CICVyA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina; CONICET, Argentina., Bigi F; Instituto de Agrobiotecnología y Biotecnología Molecular (IABIMO), UEDD INTA-CONICET; CICVyA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina; CONICET, Argentina., Zumárraga MJ; Instituto de Agrobiotecnología y Biotecnología Molecular (IABIMO), UEDD INTA-CONICET; CICVyA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina; CONICET, Argentina., Cataldi AA; Instituto de Agrobiotecnología y Biotecnología Molecular (IABIMO), UEDD INTA-CONICET; CICVyA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina; CONICET, Argentina., Eirin ME; Instituto de Agrobiotecnología y Biotecnología Molecular (IABIMO), UEDD INTA-CONICET; CICVyA, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina; CONICET, Argentina. Electronic address: eirin.maria@inta.gob.ar. |
| المصدر: | Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2022 May; Vol. 134, pp. 102203. Date of Electronic Publication: 2022 Mar 29. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Edinburgh, New York : Churchill Livingstone, c2001- |
| مواضيع طبية MeSH: | Mycobacterium bovis* , Mycobacterium tuberculosis* , Tuberculosis*/prevention & control , Tuberculosis, Bovine*/prevention & control, Animals ; BCG Vaccine ; Cattle ; Disease Models, Animal ; Mice ; Mice, Inbred BALB C |
| مستخلص: | Cattle vaccination is an attractive approach in compliance with control and eradication programs against Bovine Tuberculosis (bTB). Today, there is no anti bTB vaccine licensed. Two vaccine candidates, MbΔmce2 and MbΔmce2-phoP previously designed were evaluated in BALB/c mice, including the parental M. bovis NCTC10772 and a M. bovis hypervirulent Mb04-303 strains as controls. Sentinel mice (non-inoculated) cohoused with subcutaneous inoculated mice. Persistence, visible tuberculosis lesions (VTL) in lungs and spleens and bacillary load were investigated subcutaneously delivered at 60 and 90 days after inoculation (dpi) as well as their potential transmission to naïve mice. While a 100% survival was observed at 90 dpi without VTL in all groups, transmission was not evidenced in the sentinels mice. Vaccine candidates and control strains were isolated from the spleen of all inoculated mice, while Mb04-303 was isolated from the lungs of one inoculated mouse. Vaccine candidate's attenuation considering survival, lung bacillary load and VTL was confirmed, administrated by the subcutaneous route. Future experiments are necessary to demonstrate whether the persistence of both mutants in the spleen, with low CFU, remains over time to increase the potential increasing risk of dissemination to organs and subsequent transmission to other animals by airborne or other routes. (Copyright © 2022 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: M. bovis vaccine candidates; MbΔmce2; MbΔmce2-phoP; Mice; Persistence; Transmissibility |
| المشرفين على المادة: | 0 (BCG Vaccine) |
| تواريخ الأحداث: | Date Created: 20220403 Date Completed: 20220602 Latest Revision: 20220614 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.tube.2022.102203 |
| PMID: | 35367869 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-281X |
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| DOI: | 10.1016/j.tube.2022.102203 |