دورية أكاديمية
أعرض في EDS

Transcriptomic analysis of cumulus cells shows altered pathways in patients with minimal and mild endometriosis.

التفاصيل البيبلوغرافية
العنوان: Transcriptomic analysis of cumulus cells shows altered pathways in patients with minimal and mild endometriosis.
المؤلفون: Da Luz CM; Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, Ribeirão Preto, São Paulo, 14049-900, Brazil. carollinemantovani@gmail.com.; National Institute of Hormones and Women's Health, CNPq, Porto Alegre, Rio Grande Do Sul, 90035-003, Brazil. carollinemantovani@gmail.com., Da Broi MG; Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, Ribeirão Preto, São Paulo, 14049-900, Brazil.; National Institute of Hormones and Women's Health, CNPq, Porto Alegre, Rio Grande Do Sul, 90035-003, Brazil., Koopman LO; Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, Ribeirão Preto, São Paulo, 14049-900, Brazil.; National Institute of Hormones and Women's Health, CNPq, Porto Alegre, Rio Grande Do Sul, 90035-003, Brazil., Plaça JR; Center for Integrative Systems Biology - CISBi, NAP/USP, Ribeirão Preto, São Paulo, 14049-900, Brazil., da Silva-Jr WA; Center for Integrative Systems Biology - CISBi, NAP/USP, Ribeirão Preto, São Paulo, 14049-900, Brazil.; Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil., Ferriani RA; Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, Ribeirão Preto, São Paulo, 14049-900, Brazil.; National Institute of Hormones and Women's Health, CNPq, Porto Alegre, Rio Grande Do Sul, 90035-003, Brazil., Meola J; Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, Ribeirão Preto, São Paulo, 14049-900, Brazil.; National Institute of Hormones and Women's Health, CNPq, Porto Alegre, Rio Grande Do Sul, 90035-003, Brazil., Navarro PA; Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, Ribeirão Preto, São Paulo, 14049-900, Brazil.; National Institute of Hormones and Women's Health, CNPq, Porto Alegre, Rio Grande Do Sul, 90035-003, Brazil.
المصدر: Scientific reports [Sci Rep] 2022 Apr 06; Vol. 12 (1), pp. 5775. Date of Electronic Publication: 2022 Apr 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Endometriosis*/metabolism , Infertility, Female*/metabolism, Case-Control Studies ; Cumulus Cells/metabolism ; Female ; Humans ; Male ; Oocytes/metabolism ; Transcriptome
مستخلص: Endometriosis is a chronic inflammatory disorder that is highly associated with infertility. This association seems to be related to oocyte impairment, mainly in the initial stages of endometriosis (minimal and mild), where no distortions or adhesions are present. Nonetheless, invasive oocyte analyses are not routinely feasible; thus, indirect assessment of oocyte quality is highly desirable, and, in this context, cumulus cells (CCs) may be more suitable targets of analysis. CCs are crucial in oocyte development and could be used as an index of oocyte quality. Therefore, this prospective case-control study aimed to shed light on the infertility mechanisms of endometriosis I/II by analyzing the CCs' mRNA transcription profile (women with endometriosis I/II, n = 9) compared to controls (women with tubal abnormalities or male factor, n = 9). The transcriptomic analyses of CCs from patients with minimal and mild endometriosis revealed 26 differentially expressed genes compared to the controls. The enrichment analysis evidenced some altered molecular processes: Cytokine-cytokine receptor interactions, Chemokine signaling, TNF signaling, NOD-like receptor signaling, NF-kappa B signaling, and inflammatory response. With the exception of CXCL12, all enriched genes were downregulated in CCs from patients with endometriosis. These findings provide a significant achievement in the field of reproductive biology, directing future studies to discover biomarkers of oocyte quality in endometriosis.
(© 2022. The Author(s).)
References: J Assist Reprod Genet. 1998 Apr;15(4):193-7. (PMID: 9565848)
Lancet. 2004 Nov 13-19;364(9447):1789-99. (PMID: 15541453)
Clin Dev Immunol. 2012;2012:606459. (PMID: 22007253)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10501-6. (PMID: 10468638)
J Assist Reprod Genet. 1997 Mar;14(3):152-6. (PMID: 9090558)
Mol Reprod Dev. 2002 Mar;61(3):414-24. (PMID: 11835587)
Redox Biol. 2020 Feb;30:101431. (PMID: 31972508)
J Assist Reprod Genet. 2014 Oct;31(10):1277-85. (PMID: 25113618)
J Endocrinol. 2004 Feb;180(2):203-12. (PMID: 14765973)
Gynecol Obstet Invest. 2002;53 Suppl 1:46-51. (PMID: 11834868)
Development. 2003 May;130(10):2253-61. (PMID: 12668637)
Biol Reprod. 2008 Aug;79(2):301-9. (PMID: 18417713)
Endocrinology. 2009 Jul;150(7):3360-8. (PMID: 19299453)
Reprod Biomed Online. 2021 May;42(5):952-962. (PMID: 33736992)
Reprod Sci. 2015 Nov;22(11):1452-60. (PMID: 25947891)
PLoS One. 2011;6(11):e27179. (PMID: 22087263)
Hum Reprod. 2014 Feb;29(2):315-23. (PMID: 24166595)
Hum Reprod. 1999 Jan;14(1):162-6. (PMID: 10374114)
Reprod Biomed Online. 2015 May;30(5):532-41. (PMID: 25773531)
Hum Reprod. 1999 Dec;14 Suppl 2:77-89. (PMID: 10690803)
Rev Bras Ginecol Obstet. 2010 Jun;32(6):279-85. (PMID: 20945013)
Hum Reprod. 1994 Apr;9(4):725-9. (PMID: 8046030)
Theriogenology. 2018 Feb;107:85-94. (PMID: 29132039)
Fertil Steril. 2009 Nov;92(5):1749-52. (PMID: 19523612)
Reprod Suppl. 2003;61:49-54. (PMID: 14635926)
Clin Obstet Gynecol. 2010 Jun;53(2):429-38. (PMID: 20436320)
Mol Reprod Dev. 2018 Feb;85(2):128-136. (PMID: 29247565)
Cell Tissue Res. 2016 Oct;366(1):231-42. (PMID: 27250533)
Biol Reprod. 2008 Aug;79(2):209-22. (PMID: 18417710)
Hum Reprod. 2010 May;25(5):1259-70. (PMID: 20228394)
Fertil Steril. 1997 May;67(5):817-21. (PMID: 9130884)
BMC Genomics. 2020 Apr 19;21(1):312. (PMID: 32306892)
Reprod Sci. 2017 Sep;24(9):1304-1311. (PMID: 28110632)
Hum Reprod. 2008 May;23(5):1118-27. (PMID: 18310048)
J Pathol. 2008 Jan;214(2):149-60. (PMID: 18161752)
Cell Tissue Res. 2016 Apr;364(1):1-7. (PMID: 26685866)
Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886)
Reprod Biomed Online. 2017 Oct;35(4):379-386. (PMID: 28734688)
J Assist Reprod Genet. 2010 Aug;27(8):441-7. (PMID: 20574791)
Mol Hum Reprod. 1996 Aug;2(8):541-8. (PMID: 9239665)
Fertil Steril. 2002 Jun;77(6):1148-55. (PMID: 12057720)
Hum Reprod. 2019 Jul 8;34(7):1302-1312. (PMID: 31211846)
Mol Hum Reprod. 2008 Mar;14(3):157-68. (PMID: 18204071)
Hum Reprod. 2012 Aug;27(8):2438-47. (PMID: 22617121)
Fertil Steril. 2012 Sep;98(3):511-9. (PMID: 22819144)
Mol Cell Endocrinol. 1998 Oct 25;145(1-2):47-54. (PMID: 9922098)
J Assist Reprod Genet. 2008 Jun;25(6):223-8. (PMID: 18509754)
Mol Reprod Dev. 2004 Nov;69(3):347-55. (PMID: 15349847)
Hum Reprod. 2006 Jul;21(7):1705-19. (PMID: 16571642)
Hum Reprod. 2004 Dec;19(12):2869-74. (PMID: 15471935)
Fertil Steril. 1998 Sep;70(3):425-31. (PMID: 9757870)
J Assist Reprod Genet. 2018 May;35(5):735-751. (PMID: 29497954)
J Cell Sci. 2003 May 15;116(Pt 10):1863-73. (PMID: 12692188)
Sci Rep. 2020 Jan 15;10(1):313. (PMID: 31941945)
Mol Hum Reprod. 2010 Aug;16(8):531-8. (PMID: 20435608)
تواريخ الأحداث: Date Created: 20220407 Date Completed: 20220408 Latest Revision: 20220519
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8986826
DOI: 10.1038/s41598-022-09386-4
PMID: 35388025
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-022-09386-4