دورية أكاديمية
أعرض في EDS

Sperm Heterogeneity Accounts for Sperm DNA Methylation Variations Observed in the Caput Epididymis, Independently From DNMT/TET Activities.

التفاصيل البيبلوغرافية
العنوان: Sperm Heterogeneity Accounts for Sperm DNA Methylation Variations Observed in the Caput Epididymis, Independently From DNMT/TET Activities.
المؤلفون: Chen H; Faculty of Medicine, Université Laval, Quebec, QC, Canada.; Center for Research in Reproduction, Development and Intergenerational Health, Quebec, QC, Canada., Scott-Boyer MP; Faculty of Medicine, Université Laval, Quebec, QC, Canada., Droit A; Faculty of Medicine, Université Laval, Quebec, QC, Canada., Robert C; Center for Research in Reproduction, Development and Intergenerational Health, Quebec, QC, Canada.; Faculty of Animal Sciences, Université Laval, Quebec, QC, Canada., Belleannée C; Faculty of Medicine, Université Laval, Quebec, QC, Canada.; Center for Research in Reproduction, Development and Intergenerational Health, Quebec, QC, Canada.
المصدر: Frontiers in cell and developmental biology [Front Cell Dev Biol] 2022 Mar 22; Vol. 10, pp. 834519. Date of Electronic Publication: 2022 Mar 22 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101630250 Publication Model: eCollection Cited Medium: Print ISSN: 2296-634X (Print) Linking ISSN: 2296634X NLM ISO Abbreviation: Front Cell Dev Biol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2013]-
مستخلص: Following their production in the testis, spermatozoa enter the epididymis where they gain their motility and fertilizing abilities. This post-testicular maturation coincides with sperm epigenetic profile changes that influence progeny outcome. While recent studies highlighted the dynamics of small non-coding RNAs in maturing spermatozoa, little is known regarding sperm methylation changes and their impact at the post-fertilization level. Fluorescence-activated cell sorting (FACS) was used to purify spermatozoa from the testis and different epididymal segments (i.e., caput, corpus and cauda ) of CAG/su9-DsRed2; Acr3-EGFP transgenic mice in order to map out sperm methylome dynamics. Reduced representation bisulfite sequencing (RRBS-Seq) performed on DNA from these respective sperm populations indicated that high methylation changes were observed between spermatozoa from the caput vs. testis with 5,546 entries meeting our threshold values (q value <0.01, methylation difference above 25%). Most of these changes were transitory during epididymal sperm maturation according to the low number of entries identified between spermatozoa from cauda vs. testis. According to enzymatic and sperm/epididymal fluid co-incubation assays, (de)methylases were not found responsible for these sperm methylation changes. Instead, we identified that a subpopulation of caput spermatozoa displayed distinct methylation marks that were susceptible to sperm DNAse treatment and accounted for the DNA methylation profile changes observed in the proximal epididymis. Our results support the paradigm that a fraction of caput spermatozoa has a higher propensity to bind extracellular DNA, a phenomenon responsible for the sperm methylome variations observed at the post-testicular level. Further investigating the degree of conservation of this sperm heterogeneity in human will eventually provide new considerations regarding sperm selection procedures used in fertility clinics.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Chen, Scott-Boyer, Droit, Robert and Belleannée.)
References: Epigenetics Chromatin. 2017 May 8;10:23. (PMID: 28503201)
Microsc Res Tech. 2003 May 1;61(1):88-102. (PMID: 12672125)
Biol Reprod. 2021 Sep 14;105(3):570-592. (PMID: 33929020)
Cold Spring Harb Perspect Biol. 2014 May 01;6(5):a019133. (PMID: 24789823)
Reproduction. 2012 Jun;143(6):759-71. (PMID: 22457431)
Genes Dev. 2016 Apr 1;30(7):733-50. (PMID: 27036965)
J Cell Sci. 2001 May;114(Pt 9):1665-75. (PMID: 11309198)
Hum Reprod Update. 2016 Sep;22(5):574-87. (PMID: 27307387)
Proc Natl Acad Sci U S A. 2021 Jan 5;118(1):. (PMID: 33372158)
Mol Cell Proteomics. 2019 Mar 15;18(Suppl 1):S91-S108. (PMID: 30213844)
Reprod Biomed Online. 2006 May;12(5):630-3. (PMID: 16790111)
J Cell Biol. 1999 Oct 4;147(1):25-32. (PMID: 10508852)
Andrologia. 2013 Dec;45(6):424-9. (PMID: 23121197)
Nat Genet. 1992 Apr;1(1):40-4. (PMID: 1363808)
Mol Cell Biol. 2005 Sep;25(17):7637-44. (PMID: 16107710)
Front Endocrinol (Lausanne). 2018 Feb 28;9:59. (PMID: 29541061)
PLoS Genet. 2021 Mar 4;17(3):e1009416. (PMID: 33661909)
Front Cell Dev Biol. 2018 May 15;6:50. (PMID: 29868581)
Reproduction. 2011 May;141(5):653-63. (PMID: 21310816)
Ann N Y Acad Sci. 2005 Dec;1061:226-42. (PMID: 16467272)
Nat Genet. 1994 May;7(1):59-63. (PMID: 8075642)
Nature. 2012 Mar 28;484(7394):339-44. (PMID: 22456710)
Mol Hum Reprod. 2015 Apr;21(4):313-9. (PMID: 25542834)
Epigenetics. 2011 Nov;6(11):1354-61. (PMID: 22048249)
Asian J Androl. 2007 Jul;9(4):500-7. (PMID: 17589787)
Biol Reprod. 2016 Jul;95(1):10. (PMID: 27122633)
Int J Mol Sci. 2020 Jul 29;21(15):. (PMID: 32751076)
Nat Rev Genet. 2010 Mar;11(3):204-20. (PMID: 20142834)
Exp Anim. 2010;59(1):105-7. (PMID: 20224175)
Dev Biol. 2012 Sep 1;369(1):43-53. (PMID: 22750823)
Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
Nature. 2004 Jun 24;429(6994):900-3. (PMID: 15215868)
Theriogenology. 2006 Jul 1;66(1):14-24. (PMID: 16620928)
Hum Reprod. 2016 Jul;31(7):1411-24. (PMID: 27141042)
Mol Hum Reprod. 2020 Jan 1;26(1):14-29. (PMID: 31778536)
Nature. 1991 May 9;351(6322):153-5. (PMID: 1709450)
J Assist Reprod Genet. 2009 Sep-Oct;26(9-10):537-44. (PMID: 19876730)
BMC Genomics. 2019 Dec 6;20(1):940. (PMID: 31810461)
Front Med. 2021 Sep 13;:. (PMID: 34515940)
Cell. 2015 Jun 4;161(6):1453-67. (PMID: 26046444)
Front Cell Dev Biol. 2019 Oct 09;7:215. (PMID: 31681757)
Nat Commun. 2019 Jan 18;10(1):305. (PMID: 30659182)
Biophys J. 2017 Nov 7;113(9):1925-1933. (PMID: 29117517)
Hum Reprod Update. 2021 Dec 21;28(1):51-66. (PMID: 34618012)
Dev Biol. 2019 Jan 15;445(2):280-293. (PMID: 30500333)
Science. 2016 Nov 18;354(6314):909-912. (PMID: 27856912)
Reproduction. 2013 Oct 03;146(5):481-9. (PMID: 23988666)
PLoS One. 2019 Apr 4;14(4):e0214368. (PMID: 30946758)
Biol Reprod. 2012 Oct 18;87(4):94. (PMID: 22875906)
Genome Biol. 2012 Oct 03;13(10):R87. (PMID: 23034086)
Bioinformatics. 2011 Jun 1;27(11):1571-2. (PMID: 21493656)
Nat Rev Genet. 2013 Mar;14(3):204-20. (PMID: 23400093)
فهرسة مساهمة: Keywords: DNA methylation; RRBS (reduced representation bisulphite sequencing); epididymis; epigenetics; methyltransferase; spermatozoa
تواريخ الأحداث: Date Created: 20220408 Latest Revision: 20220409
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8981467
DOI: 10.3389/fcell.2022.834519
PMID: 35392175
قاعدة البيانات: MEDLINE
الوصف
تدمد:2296-634X
DOI:10.3389/fcell.2022.834519