دورية أكاديمية
Sperm Heterogeneity Accounts for Sperm DNA Methylation Variations Observed in the Caput Epididymis, Independently From DNMT/TET Activities.
العنوان: | Sperm Heterogeneity Accounts for Sperm DNA Methylation Variations Observed in the Caput Epididymis, Independently From DNMT/TET Activities. |
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المؤلفون: | Chen H; Faculty of Medicine, Université Laval, Quebec, QC, Canada.; Center for Research in Reproduction, Development and Intergenerational Health, Quebec, QC, Canada., Scott-Boyer MP; Faculty of Medicine, Université Laval, Quebec, QC, Canada., Droit A; Faculty of Medicine, Université Laval, Quebec, QC, Canada., Robert C; Center for Research in Reproduction, Development and Intergenerational Health, Quebec, QC, Canada.; Faculty of Animal Sciences, Université Laval, Quebec, QC, Canada., Belleannée C; Faculty of Medicine, Université Laval, Quebec, QC, Canada.; Center for Research in Reproduction, Development and Intergenerational Health, Quebec, QC, Canada. |
المصدر: | Frontiers in cell and developmental biology [Front Cell Dev Biol] 2022 Mar 22; Vol. 10, pp. 834519. Date of Electronic Publication: 2022 Mar 22 (Print Publication: 2022). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101630250 Publication Model: eCollection Cited Medium: Print ISSN: 2296-634X (Print) Linking ISSN: 2296634X NLM ISO Abbreviation: Front Cell Dev Biol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2013]- |
مستخلص: | Following their production in the testis, spermatozoa enter the epididymis where they gain their motility and fertilizing abilities. This post-testicular maturation coincides with sperm epigenetic profile changes that influence progeny outcome. While recent studies highlighted the dynamics of small non-coding RNAs in maturing spermatozoa, little is known regarding sperm methylation changes and their impact at the post-fertilization level. Fluorescence-activated cell sorting (FACS) was used to purify spermatozoa from the testis and different epididymal segments (i.e., caput, corpus and cauda ) of CAG/su9-DsRed2; Acr3-EGFP transgenic mice in order to map out sperm methylome dynamics. Reduced representation bisulfite sequencing (RRBS-Seq) performed on DNA from these respective sperm populations indicated that high methylation changes were observed between spermatozoa from the caput vs. testis with 5,546 entries meeting our threshold values (q value <0.01, methylation difference above 25%). Most of these changes were transitory during epididymal sperm maturation according to the low number of entries identified between spermatozoa from cauda vs. testis. According to enzymatic and sperm/epididymal fluid co-incubation assays, (de)methylases were not found responsible for these sperm methylation changes. Instead, we identified that a subpopulation of caput spermatozoa displayed distinct methylation marks that were susceptible to sperm DNAse treatment and accounted for the DNA methylation profile changes observed in the proximal epididymis. Our results support the paradigm that a fraction of caput spermatozoa has a higher propensity to bind extracellular DNA, a phenomenon responsible for the sperm methylome variations observed at the post-testicular level. Further investigating the degree of conservation of this sperm heterogeneity in human will eventually provide new considerations regarding sperm selection procedures used in fertility clinics. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Chen, Scott-Boyer, Droit, Robert and Belleannée.) |
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فهرسة مساهمة: | Keywords: DNA methylation; RRBS (reduced representation bisulphite sequencing); epididymis; epigenetics; methyltransferase; spermatozoa |
تواريخ الأحداث: | Date Created: 20220408 Latest Revision: 20220409 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC8981467 |
DOI: | 10.3389/fcell.2022.834519 |
PMID: | 35392175 |
قاعدة البيانات: | MEDLINE |
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