دورية أكاديمية
Altered Spinal Homeostasis and Maladaptive Plasticity in GFAP Null Mice Following Peripheral Nerve Injury.
العنوان: | Altered Spinal Homeostasis and Maladaptive Plasticity in GFAP Null Mice Following Peripheral Nerve Injury. |
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المؤلفون: | De Luca C; Neural Network Morphology & Systems Biology Lab, Division of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Virtuoso A; Neural Network Morphology & Systems Biology Lab, Division of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Korai SA; Neural Network Morphology & Systems Biology Lab, Division of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Cirillo R; Neural Network Morphology & Systems Biology Lab, Division of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Gargano F; Unit of Anesthesia, Intensive Care and Pain Management, Department of Medicine, Campus Bio-Medico University of Rome, 00128 Rome, Italy., Papa M; Neural Network Morphology & Systems Biology Lab, Division of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy.; SYSBIO Centre of Systems Biology ISBE.ITALY, University of Milano-Bicocca, 20126 Milano, Italy., Cirillo G; Neural Network Morphology & Systems Biology Lab, Division of Human Anatomy, Department of Mental and Physical Health and Preventive Medicine, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy. |
المصدر: | Cells [Cells] 2022 Apr 05; Vol. 11 (7). Date of Electronic Publication: 2022 Apr 05. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI |
مواضيع طبية MeSH: | Peripheral Nerve Injuries*, Animals ; Gliosis/metabolism ; Glutamic Acid/metabolism ; Homeostasis ; Hyperalgesia ; Mice ; Mice, Knockout ; Neuroglia/metabolism ; Vimentin |
مستخلص: | The maladaptive response of the central nervous system (CNS) following nerve injury is primarily linked to the activation of glial cells (reactive gliosis) that produce an inflammatory reaction and a wide cellular morpho-structural and functional/metabolic remodeling. Glial acidic fibrillary protein (GFAP), a major protein constituent of astrocyte intermediate filaments (IFs), is the hallmark of the reactive astrocytes, has pleiotropic functions and is significantly upregulated in the spinal cord after nerve injury. Here, we investigated the specific role of GFAP in glial reaction and maladaptive spinal cord plasticity following sciatic nerve spared nerve injury (SNI) in GFAP KO and wild-type (WT) animals. We evaluated the neuropathic behavior (thermal hyperalgesia, allodynia) and the expression of glial (vimentin, Iba1) and glutamate/GABA system markers (GLAST, GLT1, EAAC1, vGLUT, vGAT, GAD) in lumbar spinal cord sections of KO/WT animals. SNI induced neuropathic behavior in both GFAP KO and WT mice, paralleled by intense microglial reaction (Iba1 expression more pronounced in KO mice), reactive astrocytosis (vimentin increase) and expression remodeling of glial/neuronal glutamate/GABA transporters. In conclusion, it is conceivable that the lack of GFAP could be detrimental to the CNS as it lacks a critical sensor for neuroinflammation and morpho-functional-metabolic rewiring after nerve injury. Understanding the maladaptive morpho-functional changes of glial cells could represent the first step for a new glial-based targeted approach for mechanisms of disease in the CNS. |
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فهرسة مساهمة: | Keywords: GFAP; neuropathic behavior; peripheral nerve injury; reactive astrogliosis; spinal cord |
المشرفين على المادة: | 0 (Vimentin) 3KX376GY7L (Glutamic Acid) |
تواريخ الأحداث: | Date Created: 20220412 Date Completed: 20220413 Latest Revision: 20220516 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC8997460 |
DOI: | 10.3390/cells11071224 |
PMID: | 35406788 |
قاعدة البيانات: | MEDLINE |
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