دورية أكاديمية
Cloning, expression and sequencing the molybdenum-pterin binding protein (mop) gene of Clostridium pasteurianum in Escherichia coli.
| العنوان: | Cloning, expression and sequencing the molybdenum-pterin binding protein (mop) gene of Clostridium pasteurianum in Escherichia coli. |
|---|---|
| المؤلفون: | Hinton SM, Freyer G |
| المصدر: | Nucleic acids research [Nucleic Acids Res] 1986 Dec 09; Vol. 14 (23), pp. 9371-80. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Print ISSN: 0305-1048 (Print) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1992- : Oxford : Oxford University Press Original Publication: London, Information Retrieval ltd. |
| مواضيع طبية MeSH: | Cloning, Molecular* , Coenzymes* , Genes, Bacterial* , Metalloproteins*, Bacterial Proteins/*genetics , Carrier Proteins/*genetics , Clostridium/*genetics , Escherichia coli/*genetics , Molybdenum/*metabolism , Pteridines/*metabolism, Amino Acid Sequence ; Bacterial Proteins/analysis ; Bacterial Proteins/physiology ; Base Sequence ; Carrier Proteins/analysis ; Carrier Proteins/physiology ; Chromosome Mapping ; DNA, Bacterial/analysis ; DNA-Binding Proteins/analysis ; Molybdenum Cofactors ; Pterins |
| مستخلص: | mop is the structural gene for the molybdenum-pterin binding protein, which is the major molybdenum binding protein in Clostridium pastuerianum. The mop gene was detected by immunoscreening genomic libraries of C. pastuerianum and identified by determining the nucleotide sequence of the cloned insert of clostridial DNA. The deduced amino acid sequence of an open reading frame proved to be identical to the first twelve residues of purified Mop. The DNA sequence flanking the mop gene contains promoter-like consensus sequences which are probably responsible for the expression of Mop in Escherichia coli. The deduced amino acid composition shows that the protein is hydrophobic, lacks aromatic and cysteine residues and has a calculated molecular weight of 7,038. The N-terminal amino acid sequence of Mop has sequence homology with DNA binding proteins. The pattern and type of residues in the N-terminal region suggest it forms the helix-turn-helix structure observed in DNA binding proteins. We propose that Mop may be a regulatory protein binding the anabolic source of molybdenum. |
| References: | Nature. 1970 Aug 15;227(5259):680-5. (PMID: 5432063) Gene. 1982 Oct;19(3):269-76. (PMID: 6295880) Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148. (PMID: 364941) Proc Natl Acad Sci U S A. 1983 Jan;80(1):31-5. (PMID: 6185958) J Mol Biol. 1983 May 15;166(2):241-7. (PMID: 6343617) J Mol Biol. 1983 Jun 5;166(4):557-80. (PMID: 6345791) Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. (PMID: 6575390) Nucleic Acids Res. 1983 Aug 25;11(16):5521-40. (PMID: 6310503) J Bacteriol. 1985 May;162(2):485-93. (PMID: 3857224) Anal Biochem. 1985 Mar;145(2):222-9. (PMID: 4014654) J Bacteriol. 1986 Nov;168(2):688-93. (PMID: 3782020) Nucleic Acids Res. 1979 Nov 24;7(6):1513-23. (PMID: 388356) J Biol Chem. 1980 Mar 10;255(5):1783-6. (PMID: 6892571) Annu Rev Genet. 1979;13:319-53. (PMID: 94251) Nature. 1982 Jul 29;298(5873):447-51. (PMID: 6896364) Cell. 1982 Aug;30(1):45-52. (PMID: 6127165) Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. (PMID: 271968) |
| سلسلة جزيئية: | GENBANK X04982 |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Carrier Proteins) 0 (Coenzymes) 0 (DNA, Bacterial) 0 (DNA-Binding Proteins) 0 (Metalloproteins) 0 (Molybdenum Cofactors) 0 (Pteridines) 0 (Pterins) 0 (mopI protein, Clostridium pasteurianum) 81AH48963U (Molybdenum) ATN6EG42UQ (molybdenum cofactor) |
| تواريخ الأحداث: | Date Created: 19861209 Date Completed: 19870218 Latest Revision: 20211203 |
| رمز التحديث: | 20221208 |
| مُعرف محوري في PubMed: | PMC311964 |
| DOI: | 10.1093/nar/14.23.9371 |
| PMID: | 3540853 |
| قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!