دورية أكاديمية
An optimized protocol for phenotyping human granulocytes by mass cytometry.
| العنوان: | An optimized protocol for phenotyping human granulocytes by mass cytometry. |
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| المؤلفون: | Vivanco Gonzalez N; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States., Oliveria JP; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.; Department of Medicine, Division of Respirology, McMaster University, Hamilton, ON, L8S4K1, Canada., Tebaykin D; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States., Ivison GT; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States., Mukai K; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.; Sean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States., Tsai MM; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.; Sean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States., Borges L; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States., Nadeau KC; Sean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States.; Department of Medicine, Division of Pulmonary and Critical Care Medicine, School of Medicine, Stanford University, Stanford, CA, United States., Galli SJ; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.; Sean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States.; Department of Microbiology and Immunology, School of Medicine, Stanford University, Stanford, CA, United States., Tsai AG; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States., Bendall SC; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States. |
| المصدر: | STAR protocols [STAR Protoc] 2022 Apr 08; Vol. 3 (2), pp. 101280. Date of Electronic Publication: 2022 Apr 08 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Eosinophils*/metabolism , Neutrophils*, Basophils ; Flow Cytometry/methods ; Humans ; Leukocyte Count |
| مستخلص: | Granulocytes encompass diverse roles, from fighting off pathogens to regulating inflammatory processes in allergies. These roles are represented by distinct cellular phenotypes that we captured with mass cytometry (CyTOF). Our protocol enables simultaneous evaluation of human basophils, eosinophils, and neutrophils under homeostasis and upon immune activation by anti-Immunoglobulin E (anti-IgE) or interleukin-3 (IL-3). Granulocyte integrity and detection of protein markers were optimized so that rare granulocyte populations could be deeply characterized by single cell mass cytometry. For complete details on the use and execution of this protocol, please refer to Vivanco Gonzalez et al. (2020). Competing Interests: The authors declare no competing interests. (© 2022 The Authors.) |
| References: | Methods Mol Biol. 2019;1989:55-81. (PMID: 31077099) Genome Biol. 2018 Feb 6;19(1):15. (PMID: 29409532) J Allergy Clin Immunol. 2017 Mar;139(3):889-899.e11. (PMID: 27527263) Cytometry A. 2013 May;83(5):483-94. (PMID: 23512433) iScience. 2020 Oct 22;23(11):101724. (PMID: 33205028) |
| معلومات مُعتمدة: | U19 AI104209 United States AI NIAID NIH HHS; U19 AI116484 United States AI NIAID NIH HHS; R00 GM104148 United States GM NIGMS NIH HHS; U24 CA224309 United States CA NCI NIH HHS; R01 AG056287 United States AG NIA NIH HHS; R01 AG057915 United States AG NIA NIH HHS; Canada CIHR |
| فهرسة مساهمة: | Keywords: Antibody; Cell isolation; Flow Cytometry/Mass Cytometry; Health Sciences; Immunology; Single Cell |
| تواريخ الأحداث: | Date Created: 20220418 Date Completed: 20220419 Latest Revision: 20220629 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9010787 |
| DOI: | 10.1016/j.xpro.2022.101280 |
| PMID: | 35434655 |
| قاعدة البيانات: | MEDLINE |
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