دورية أكاديمية

Bioinformatics and genetic variants analysis of FGF10 gene promoter with their association at carcass quality and body measurement traits in Qinchuan beef cattle.

التفاصيل البيبلوغرافية
العنوان: Bioinformatics and genetic variants analysis of FGF10 gene promoter with their association at carcass quality and body measurement traits in Qinchuan beef cattle.
المؤلفون: Nurgulsim K; College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China.; Faculty of Veterinary and Livestock Technology, S. Seifullin Kazakh Agro technical University, Nur-Sultan, Kazakhstan., Khan R; Department of Livestock Management, Breeding and Genetic, The University of Agriculture, Peshawar, Pakistan., Raza SHA; College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China., Ayari-Akkari A; Biology Department, College of Sciences in Abha, King Khalid University, Abha, Saudi Arabia., Jeridi M; Biology Department, College of Sciences in Abha, King Khalid University, Abha, Saudi Arabia., Ahmad I; Department of Livestock Management, Breeding and Genetic, The University of Agriculture, Peshawar, Pakistan., Arain UM; Department of Poultry Husbandry, Sindh Agriculture University, Tandojam, Pakistan., Abd El-Aziz AH; Department of Animal Breeding and Production, Faculty of Veterinary Medicine, Damanhour University, Damanhur, Egypt., Khan H; College of Veterinary Sciences, The University of Agriculture Peshawar, Peshawar, Pakistan., Zan L; College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China.
المصدر: Animal biotechnology [Anim Biotechnol] 2023 Nov; Vol. 34 (6), pp. 1950-1959. Date of Electronic Publication: 2022 Apr 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 9011409 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2378 (Electronic) Linking ISSN: 10495398 NLM ISO Abbreviation: Anim Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2005- : Abingdon, Oxford : Taylor & Francis
Original Publication: New York, N.Y. : Marcel Dekker, c1990-
مواضيع طبية MeSH: Body Weights and Measures* , Polymorphism, Single Nucleotide*/genetics, Cattle/genetics ; Animals ; Phenotype ; Genotype ; Computational Biology ; Gene Frequency ; Sequence Analysis, DNA ; Meat
مستخلص: The fibroblast growth factor 10 ( FGF10 ) gene regulates adipogenesis and myogensis. In this study, sequencing of FGF10 prompter region identified three SNPs at loci g.78G > A, g.116C > T and g.201A > T. Each SNP yields three genotypes as GG, GA and AA at loci g.78G > A, CC, CT and TT at loci g.116C > T and AA, AT and TT at loci g.201A > T. Allelic and genotypic frequencies of all three SNPs deviated from the Hardy-Weinberg equilibrium (HWE) ( P  < 0.05) and were found highly polymorphic as PIC (0.25 < PIC < 0.50). Moreover, we found highest LD (D'/γ2) between SNP2 and SNP3 (0.989/0.909), followed by SNP1 and SNP3 (0.944/0.796). Moreover, three variants of FGF10 gene promoter exhibited significant (P < 0.05) association with body measurement and carcass quality traits in Qinchuan beef cattle. At loci g.78G > A, the genotype GG showed significantly (P < 0.01) larger body length (BL), rump length (RL), chest depth (CD), chest circumference (CC) and ultrasound loin area (ULA). The genotype TC at loci g.116C > T showed significantly ( P  < 0.01 and 0.05) larger body measurement and intramuscular fat, and ultrasound loin area (ULA). In addition to that, at loci g.201A > T, genotype TT showed significantly (P < 0.01 and P < 0.05) larger body length (BL), rump length (RL), hip width (HW), chest circumference (CC) and ultrasound loin area (ULA). Additionally, screening of promoter sequence of FGF10 gene explored loss of four TFs binding sites ( KLF3 , ZNF37α , GLIS2 and BCL11A ) at g.116C > T because of SNP2. However, a single TF binding site was lost at g.202A > T due to SNP3. Interestingly, none of TF binding site was lost at g.78G > A in SNP1; however, one new TF binding site was gained at this location due to SNP1. These findings conclude that genotype GG, TC and TT could be used as genetic markers of FGF10 gene for body measurement and carcass quality traits in Qinchuan beef cattle.
فهرسة مساهمة: Keywords: FGF10 gene; SNPs; meat quality; promoter region; transcription factors
تواريخ الأحداث: Date Created: 20220421 Date Completed: 20230918 Latest Revision: 20230918
رمز التحديث: 20230918
DOI: 10.1080/10495398.2022.2059667
PMID: 35446746
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-2378
DOI:10.1080/10495398.2022.2059667