دورية أكاديمية
Structure based innovative approach to analyze aptaprobe-GPC3 complexes in hepatocellular carcinoma.
العنوان: | Structure based innovative approach to analyze aptaprobe-GPC3 complexes in hepatocellular carcinoma. |
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المؤلفون: | Shin WR; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Park DY; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Kim JH; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Lee JP; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Thai NQ; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Oh IH; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Sekhon SS; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea., Choi W; Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, Jeonju, 54896, Republic of Korea., Kim SY; College of Pharmacy, Wonkwang University, Shinyoung-dong 344-2, Iksan, Jeonbuk, 570-749, Republic of Korea., Cho BK; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Kim SC; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea., Min J; Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, Jeonju, 54896, Republic of Korea. jihomin@jbnu.ac.kr., Ahn JY; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea. jyahn@chungbuk.ac.kr., Kim YH; School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Republic of Korea. kyh@chungbuk.ac.kr. |
المصدر: | Journal of nanobiotechnology [J Nanobiotechnology] 2022 Apr 27; Vol. 20 (1), pp. 204. Date of Electronic Publication: 2022 Apr 27. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : BioMed Central, 2003- |
مواضيع طبية MeSH: | Carcinoma, Hepatocellular*/pathology , Liver Neoplasms*/pathology, Glypicans/metabolism ; Humans |
مستخلص: | Background: Glypican-3 (GPC3), a membrane-bound heparan sulfate proteoglycan, is a biomarker of hepatocellular carcinoma (HCC) progression. Aptamers specifically binding to target biomolecules have recently emerged as clinical disease diagnosis targets. Here, we describe 3D structure-based aptaprobe platforms for detecting GPC3, such as aptablotting, aptaprobe-based sandwich assay (ALISA), and aptaprobe-based imaging analysis. Results: For preparing the aptaprobe-GPC3 platforms, we obtained 12 high affinity aptamer candidates (GPC3_1 to GPC3_12) that specifically bind to target GPC3 molecules. Structure-based molecular interactions identified distinct aptatopic residues responsible for binding to the paratopic nucleotide sequences (nt-paratope) of GPC3 aptaprobes. Sandwichable and overlapped aptaprobes were selected through structural analysis. The aptaprobe specificity for using in HCC diagnostics were verified through Aptablotting and ALISA. Moreover, aptaprobe-based imaging showed that the binding property of GPC3_3 and their GPC3 specificity were maintained in HCC xenograft models, which may indicate a new HCC imaging diagnosis. Conclusion: Aptaprobe has the potential to be used as an affinity reagent to detect the target in vivo and in vitro diagnosing system. (© 2022. The Author(s).) |
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معلومات مُعتمدة: | NRF-2020R1A2C1009463 National Research Foundation of Korea; 2020R1A6A1A06046235 National Research Foundation of Korea; MAFRA_321108-04 Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries |
فهرسة مساهمة: | Keywords: ALISA; Aptablotting; Aptaprobe; Aptaprobe-based imaging; Cancer diagnosis; Glypican-3; Hepatocellular carcinoma; Structure-based molecular interaction |
المشرفين على المادة: | 0 (GPC3 protein, human) 0 (Glypicans) |
تواريخ الأحداث: | Date Created: 20220428 Date Completed: 20220429 Latest Revision: 20220716 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC9044640 |
DOI: | 10.1186/s12951-022-01391-z |
PMID: | 35477501 |
قاعدة البيانات: | MEDLINE |
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