دورية أكاديمية
أعرض في EDS

Functional identification of microRNA-centered complexes in C. elegans.

التفاصيل البيبلوغرافية
العنوان: Functional identification of microRNA-centered complexes in C. elegans.
المؤلفون: Hebbar S; Division of Biology, Kansas State University, Manhattan, 66506, USA., Panzade G; Division of Biology, Kansas State University, Manhattan, 66506, USA., Vashisht AA; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, 90095, USA.; Genomics Institute of the Novartis Research Foundation, San Diego, 92121, USA., Wohlschlegel JA; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, 90095, USA., Veksler-Lublinsky I; Department of Software and Information Systems Engineering, Ben-Gurion University of the Negev, 8410501, Beer-Sheva, Israel., Zinovyeva AY; Division of Biology, Kansas State University, Manhattan, 66506, USA. Zinovyeva@ksu.edu.
المصدر: Scientific reports [Sci Rep] 2022 May 03; Vol. 12 (1), pp. 7133. Date of Electronic Publication: 2022 May 03.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Caenorhabditis elegans Proteins*/genetics , Caenorhabditis elegans Proteins*/metabolism , MicroRNAs*/metabolism, Animals ; Caenorhabditis elegans/metabolism ; RNA Interference ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism
مستخلص: microRNAs (miRNAs) are crucial for normal development and physiology. To identify factors that might coordinate with miRNAs to regulate gene expression, we used 2'O-methylated oligonucleotides to precipitate Caenorhabditis elegans let-7, miR-58, and miR-2 miRNAs and the associated proteins. A total of 211 proteins were identified through mass-spectrometry analysis of miRNA co-precipitates, which included previously identified interactors of key miRNA pathway components. Gene ontology analysis of the identified interactors revealed an enrichment for RNA binding proteins, suggesting that we captured proteins that may be involved in mRNA lifecycle. To determine which miRNA interactors are important for miRNA activity, we used RNAi to deplete putative miRNA co-factors in animals with compromised miRNA activity and looked for alterations of the miRNA mutant phenotypes. Depletion of 25 of 39 tested genes modified the miRNA mutant phenotypes in three sensitized backgrounds. Modulators of miRNA phenotypes ranged from RNA binding proteins RBD-1 and CEY-1 to metabolic factors such as DLST-1 and ECH-5, among others. The observed functional interactions suggest widespread coordination of these proteins with miRNAs to ultimately regulate gene expression. This study provides a foundation for future investigations aimed at deciphering the molecular mechanisms of miRNA-mediated gene regulation.
(© 2022. The Author(s).)
References: Elife. 2017 May 02;6:. (PMID: 28463115)
Nucleic Acids Res. 2017 Feb 28;45(4):2081-2098. (PMID: 28204614)
Mol Cell. 2008 Nov 21;32(4):592-9. (PMID: 19026789)
Genome Res. 2015 Nov;25(11):1680-91. (PMID: 26232411)
Biochem Soc Trans. 2014 Aug;42(4):1135-40. (PMID: 25110015)
Mol Cell. 2005 Aug 19;19(4):437-47. (PMID: 16109369)
Sci Adv. 2020 Feb 12;6(7):eaay7667. (PMID: 32095531)
Curr Biol. 2002 Sep 3;12(17):1448-61. (PMID: 12225660)
Aging Cell. 2007 Feb;6(1):111-9. (PMID: 17266680)
Methods Mol Biol. 2011;725:233-49. (PMID: 21528458)
Nat Struct Mol Biol. 2007 Oct;14(10):934-40. (PMID: 17873886)
Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):E5271-80. (PMID: 26351692)
Biotechnol J. 2008 Jun;3(6):803-12. (PMID: 18383023)
Curr Protein Pept Sci. 2012 Jun;13(4):372-9. (PMID: 22708488)
Nature. 2003 Dec 18;426(6968):845-9. (PMID: 14685240)
Nat Struct Mol Biol. 2012 Jan 10;19(2):145-51. (PMID: 22233755)
RNA Biol. 2012 Mar;9(3):242-8. (PMID: 22336713)
Science. 2005 May 20;308(5725):1164-7. (PMID: 15790806)
Exp Neurol. 2020 Jan;323:113094. (PMID: 31676317)
Methods. 2003 Aug;30(4):313-21. (PMID: 12828945)
Nat Struct Mol Biol. 2007 Jul;14(7):591-6. (PMID: 17558416)
Nature. 2000 Feb 24;403(6772):901-6. (PMID: 10706289)
Nucleic Acids Res. 2013 Oct;41(18):8692-705. (PMID: 23863838)
Nucleic Acids Res. 2000 Sep 15;28(18):3442-4. (PMID: 10982861)
Mol Cell. 2010 Nov 24;40(4):558-70. (PMID: 21095586)
Proteins. 2009 May 1;75(2):360-72. (PMID: 18831052)
Nat Protoc. 2009;4(1):44-57. (PMID: 19131956)
Cell Syst. 2016 May 25;2(5):297-311. (PMID: 27211857)
Mol Cell. 2016 Oct 20;64(2):320-333. (PMID: 27720646)
Nucleic Acids Res. 2021 May 21;49(9):4803-4815. (PMID: 33758928)
Dev Biol. 2002 Dec 15;252(2):202-13. (PMID: 12482710)
Cell. 2006 Jun 2;125(5):887-901. (PMID: 16751099)
RNA. 2008 Dec;14(12):2580-96. (PMID: 18978028)
Nature. 2000 Nov 2;408(6808):86-9. (PMID: 11081512)
Dev Cell. 2015 Feb 9;32(3):335-44. (PMID: 25669883)
Nucleic Acids Res. 2015 Nov 16;43(20):9978-93. (PMID: 26400166)
Nature. 2011 Jan 6;469(7328):97-101. (PMID: 21085120)
Nucleic Acids Res. 2016 Jul 8;44(12):5924-35. (PMID: 27095199)
Dev Cell. 2005 Sep;9(3):403-14. (PMID: 16139228)
Dev Cell. 2003 May;4(5):625-37. (PMID: 12737799)
Nat Commun. 2015 Dec 03;6:10127. (PMID: 26632259)
J Cell Physiol. 2020 Nov;235(11):8812-8825. (PMID: 32394436)
Front Genet. 2019 May 02;10:332. (PMID: 31118942)
Cell. 2001 Jul 13;106(1):23-34. (PMID: 11461699)
J Vis Exp. 2012 Jun 10;(64):e4019. (PMID: 22710399)
Mol Cell. 2010 Jul 30;39(2):292-9. (PMID: 20605501)
J Vis Exp. 2020 May 23;(159):. (PMID: 32510481)
G3 (Bethesda). 2016 May 03;6(5):1227-37. (PMID: 26921297)
EMBO Rep. 2013 Jun;14(6):568-76. (PMID: 23619095)
PLoS Genet. 2014 Apr 24;10(4):e1004286. (PMID: 24763381)
J Proteome Res. 2002 Jan-Feb;1(1):21-6. (PMID: 12643522)
Curr Biol. 2007 Dec 4;17(23):2013-22. (PMID: 18023351)
Cell. 2009 Mar 6;136(5):926-38. (PMID: 19269369)
Nature. 2003 Sep 25;425(6956):415-9. (PMID: 14508493)
Mol Cell Proteomics. 2012 Nov;11(11):1442-56. (PMID: 22918229)
PLoS Genet. 2019 Oct 4;15(10):e1008067. (PMID: 31584932)
Dev Biol. 2016 Jan 15;409(2):459-72. (PMID: 26601717)
Nucleic Acids Res. 2012 Jun;40(11):5088-100. (PMID: 22362743)
Dev Cell. 2018 Oct 22;47(2):239-247.e4. (PMID: 30245155)
Mol Cell. 2015 Feb 19;57(4):708-720. (PMID: 25639470)
Nat Commun. 2021 Aug 20;12(1):5057. (PMID: 34417449)
Front Mol Biosci. 2021 Jun 09;8:701975. (PMID: 34235183)
Cell. 2005 Aug 26;122(4):553-63. (PMID: 16122423)
Genes Dev. 2004 Jan 15;18(2):132-7. (PMID: 14729570)
Dev Biol. 2015 May 15;401(2):276-86. (PMID: 25732775)
Biochem Soc Trans. 2021 Jun 30;49(3):1099-1108. (PMID: 34110361)
Nat Struct Mol Biol. 2014 Oct;21(10):901-10. (PMID: 25240799)
Science. 2001 Aug 3;293(5531):834-8. (PMID: 11452083)
Mol Cell. 2007 Nov 30;28(4):598-613. (PMID: 18042455)
معلومات مُعتمدة: P40 OD010440 United States OD NIH HHS; R35 GM124828 United States GM NIGMS NIH HHS; R35GM124828 United States NH NIH HHS
المشرفين على المادة: 0 (Caenorhabditis elegans Proteins)
0 (MicroRNAs)
0 (RNA-Binding Proteins)
تواريخ الأحداث: Date Created: 20220503 Date Completed: 20220505 Latest Revision: 20240513
رمز التحديث: 20240513
مُعرف محوري في PubMed: PMC9065084
DOI: 10.1038/s41598-022-10771-2
PMID: 35504914
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-022-10771-2