دورية أكاديمية
أعرض في EDS

Echinodorus macrophyllus fraction with a high level of flavonoid inhibits peripheral and central mechanisms of nociception.

التفاصيل البيبلوغرافية
العنوان: Echinodorus macrophyllus fraction with a high level of flavonoid inhibits peripheral and central mechanisms of nociception.
المؤلفون: Fernandes DC; Department of Biochemistry and Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Martins BP; Department of Biochemistry and Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Silva GPD; Department of Biochemistry and Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Fonseca END; Department of Plant Biology, Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Santos SVM; Department of Biochemistry and Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Velozo LSM; Department of Biochemistry and Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Gayer CRM; Department of Biochemistry and Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Sabino KCC; Department of Biochemistry and Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Coelho MGP; Department of Biochemistry and Institute of Biology Roberto Alcantara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
المصدر: Journal of traditional and complementary medicine [J Tradit Complement Med] 2021 Jul 15; Vol. 12 (2), pp. 123-130. Date of Electronic Publication: 2021 Jul 15 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V. on behalf of the Center for Food and Biomolecules, National Taiwan University Country of Publication: Netherlands NLM ID: 101605474 Publication Model: eCollection Cited Medium: Print ISSN: 2225-4110 (Print) Linking ISSN: 22254110 NLM ISO Abbreviation: J Tradit Complement Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier B.V. on behalf of the Center for Food and Biomolecules, National Taiwan University
Original Publication: Philadelphia, PA : [Taipei] : Wolters Kluwer Health, Medknow ; Committee on Chinese Medicine and Pharmacy
مستخلص: Background and Aim: Echinodorus macrophyllus (Kunth.) Micheli is popularly used for acute and chronic inflammatory conditions. The anti-inflammatory activity was previously demonstrated for its flavonoid-enriched fractions. The aim of this work assessed the antinociceptive properties of both aqueous extract and its fractions.
Experimental Procedure: The antinociceptive activity was determined by acetic acid-induced writhing, formalin test, tail immersion test, hot-plate test, xylene-induced ear edema methods, and the evaluation of its mechanism was performed in the writhing model. The aqueous extract of Echinodorus macrophyllus (AEEm) was fractionated, yielding Fr20, and Fr40. Fr40 composition was determined by HPLC-DAD-ESI-MS.
Results and Conclusion: Fr20 (all doses) and Fr40 (100 mg/kg) reduced the nociception in the tail-flick model. Both fractions increased the percentage of maximum possible effect with 25 mg/kg, in the hot-plate assay, at 60 min, while AEEm reduced pain only with 50 and 100 mg/kg. There was a reduction in xylene-edema index, with Fr40 (25 mg/kg), AEEm (50 mg/kg) and Fr20 (50 mg/kg). All doses of AEEm, Fr20, and Fr40 reduced both phases of the formalin model. In the abdominal contortion model, Fr40 presented the highest activity, reducing 96% of contortions and its antinociceptive mechanism was evaluated. The results indicated the involvement of NO and adrenergic activation pathways. The main components of Fr40 are swertisin, swertiajaponin, isoorientin 7,3'-dimethyl ether, swertisin-O-rhamnoside, isoorientin, isovitexin, isovitexin-Orhamnoside, and isovitexin-7-O-glucoside. The aqueous extract of E. macrophyllus leaves and its fractions exhibited significant analgesic effect, mediated through both peripheral and central mechanisms being considered a potentially antinociceptive drug.
Competing Interests: The authors declare that they have no conflicts of interest to disclose.
(© 2021 Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC.)
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فهرسة مساهمة: Keywords: 7-NI, 7-nitroindazole; AEEm, aqueous extract of E. macrophyllus; Adrenergic pathway; Chemical; DAD-UV diode array detector, ultraviolet; ESI-MS, electrospray ionization mass spectrometer; Flavonoid derivatives; Fr20, fraction isolated from EAEm on Sephadex LH-20 with 20% ethanol; Fr40, fraction isolated from EAEm on Sephadex LH-20 with 40% ethanol; GMPc, cyclic guanosine monophosphate; HPLC, high-performance liquid chromatography; HPTLC, high-performance thin-layer chromatography; MPE, percentage of the maximum possible effect; Medicinal plants; NO pathway; NO, nitric oxide; NP/PEG, natural products reagent/polyethylene glycol.; NSAIDs, nonsteroidal anti-inflammatory drugs; ODQ, 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one; Q-TOF, quadrupole time-of-flight; Thermal and neurogenic assays
تواريخ الأحداث: Date Created: 20220509 Latest Revision: 20220716
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9072821
DOI: 10.1016/j.jtcme.2021.07.001
PMID: 35528477
قاعدة البيانات: MEDLINE
الوصف
تدمد:2225-4110
DOI:10.1016/j.jtcme.2021.07.001