دورية أكاديمية
أعرض في EDS

Profiling of hMPV F-specific antibodies isolated from human memory B cells.

التفاصيل البيبلوغرافية
العنوان: Profiling of hMPV F-specific antibodies isolated from human memory B cells.
المؤلفون: Xiao X; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA.; Discovery Biologics, Merck & Co., Inc., Boston, MA, USA.; MRL Postdoctoral Research Program; Merck & Co., Inc., Kenilworth, NJ, USA., Fridman A; Data Science and Scientific Informatics, Merck & Co., Inc., Rahway, NJ, USA., Zhang L; Bioinformatics and Biomarker Research, MSD, Beijing, China., Pristatsky P; Analytical Research and Development, Merck & Co., Inc., West Point, PA, USA., Durr E; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA., Minnier M; AgileOne, Torrence, CA, USA., Tang A; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA., Cox KS; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA., Wen Z; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA., Moore R; Discovery Biologics, Merck & Co., Inc., Boston, MA, USA., Tian D; Wuxi Biortus Biosciences Co. Ltd., Wuxi, China., Galli JD; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA., Cosmi S; Eurofins PSS Insourcing Solutions, Lancaster, PA, USA., Eddins MJ; Computational and Structural Chemistry, Merck & Co., Inc., West Point, PA, USA., Sullivan NL; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA., Yan X; Wuxi Biortus Biosciences Co. Ltd., Wuxi, China., Bett AJ; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA., Su HP; Computational and Structural Chemistry, Merck & Co., Inc., West Point, PA, USA., Vora KA; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA. kalpit.vora@merck.com., Chen Z; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA. zhifeng.chen@merck.com., Zhang L; Infectious Diseases and Vaccines Discovery, Merck & Co., Inc., West Point, PA, USA. lan_zhang2@merck.com.
المصدر: Nature communications [Nat Commun] 2022 May 10; Vol. 13 (1), pp. 2546. Date of Electronic Publication: 2022 May 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Metapneumovirus* , Respiratory Syncytial Virus, Human*, Antibodies, Monoclonal ; Antibodies, Neutralizing ; Antibodies, Viral ; Epitopes ; Humans ; Memory B Cells ; Viral Fusion Proteins
مستخلص: Human metapneumovirus (hMPV) belongs to the Pneumoviridae family and is closely related to respiratory syncytial virus (RSV). The surface fusion (F) glycoprotein mediates viral fusion and is the primary target of neutralizing antibodies against hMPV. Here we report 113 hMPV-F specific monoclonal antibodies (mAbs) isolated from memory B cells of human donors. We characterize the antibodies' germline usage, epitopes, neutralization potencies, and binding specificities. We find that unlike RSV-F specific mAbs, antibody responses to hMPV F are less dominant against the apex of the antigen, and the majority of the potent neutralizing mAbs recognize epitopes on the side of hMPV F. Furthermore, neutralizing epitopes that differ from previously defined antigenic sites on RSV F are identified, and multiple binding modes of site V and II mAbs are discovered. Interestingly, mAbs that bind preferentially to the unprocessed prefusion F show poor neutralization potency. These results elucidate the immune recognition of hMPV infection and provide novel insights for future hMPV antibody and vaccine development.
(© 2022. Merck & Co., Inc., Kenilworth, NJ, USA and its affiliates.)
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المشرفين على المادة: 0 (Antibodies, Monoclonal)
0 (Antibodies, Neutralizing)
0 (Antibodies, Viral)
0 (Epitopes)
0 (Viral Fusion Proteins)
تواريخ الأحداث: Date Created: 20220510 Date Completed: 20220512 Latest Revision: 20220716
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC9091222
DOI: 10.1038/s41467-022-30205-x
PMID: 35538099
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-022-30205-x