دورية أكاديمية
Protocol for fluorescence-activated cell sorting of human EpCAM + lung cancer cells for gene expression analysis of Rac guanine-nucleotide exchange factors.
| العنوان: | Protocol for fluorescence-activated cell sorting of human EpCAM + lung cancer cells for gene expression analysis of Rac guanine-nucleotide exchange factors. |
|---|---|
| المؤلفون: | Sullivan NT; Division of Thoracic Surgery, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA., Cooke M; Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.; Department of Medicine, Einstein Medical Center Philadelphia, Philadelphia, PA 19141, USA. |
| المصدر: | STAR protocols [STAR Protoc] 2022 May 02; Vol. 3 (2), pp. 101367. Date of Electronic Publication: 2022 May 02 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Guanine Nucleotide Exchange Factors* , Lung Neoplasms*/genetics, Epithelial Cell Adhesion Molecule/genetics ; Flow Cytometry/methods ; Gene Expression ; Guanine ; Humans ; Nucleotides |
| مستخلص: | Here, we describe a protocol for fluorescence-activated cell sorting (FACS) of human EpCAM + cells from fresh surgically resected specimens. We then use Q-PCR to identify specific molecular targets associated with the metastatic phenotype. This combined approach enables a qualitative and quantitative gene expression analysis of lung cancer samples. We describe how to use the protocol for Rac GEFs, but it can be applied broadly to other molecular targets. For complete details on the use and execution of this protocol, please refer to Cooke et al. (2021) and Quatromoni et al. (2015). Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).) |
| References: | J Leukoc Biol. 2015 Jan;97(1):201-9. (PMID: 25359999) Cancer Res. 2004 Aug 15;64(16):5818-24. (PMID: 15313925) JCO Oncol Pract. 2020 Aug;16(8):e823-e828. (PMID: 32352882) Eur J Cardiothorac Surg. 2021 May 8;59(5):1014-1020. (PMID: 33332526) Cell Rep. 2021 Nov 2;37(5):109905. (PMID: 34731623) J Thorac Cardiovasc Surg. 2018 Jul;156(1):365-368. (PMID: 29921098) J Natl Compr Canc Netw. 2021 Mar 02;19(3):254-266. (PMID: 33668021) Br J Cancer. 2007 Feb 12;96(3):417-23. (PMID: 17211480) |
| معلومات مُعتمدة: | T32 CA009140 United States CA NCI NIH HHS |
| فهرسة مساهمة: | Keywords: Cancer; Cell Biology; Flow Cytometry/Mass Cytometry; Molecular Biology |
| المشرفين على المادة: | 0 (EPCAM protein, human) 0 (Epithelial Cell Adhesion Molecule) 0 (Guanine Nucleotide Exchange Factors) 0 (Nucleotides) 5Z93L87A1R (Guanine) |
| تواريخ الأحداث: | Date Created: 20220511 Date Completed: 20220512 Latest Revision: 20240513 |
| رمز التحديث: | 20240513 |
| مُعرف محوري في PubMed: | PMC9079335 |
| DOI: | 10.1016/j.xpro.2022.101367 |
| PMID: | 35542175 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
|---|---|
| DOI: | 10.1016/j.xpro.2022.101367 |