دورية أكاديمية
Self-Assembled Hydrogel Microparticle-Based Tooth-Germ Organoids.
| العنوان: | Self-Assembled Hydrogel Microparticle-Based Tooth-Germ Organoids. |
|---|---|
| المؤلفون: | Kilic Bektas C; Department of Chemistry and Chemical Biology, Rutgers University, 123 Bevier Rd, Piscataway, NJ 08854, USA., Zhang W; Division of Craniofacial and Molecular Genetics, Department of Orthodontics, Tufts University School of Dental Medicine, 1 Kneeland Avenue, Boston, MA 02111, USA., Mao Y; Department of Chemistry and Chemical Biology, Rutgers University, 123 Bevier Rd, Piscataway, NJ 08854, USA., Wu X; Department of Chemistry and Chemical Biology, Rutgers University, 123 Bevier Rd, Piscataway, NJ 08854, USA., Kohn J; Department of Chemistry and Chemical Biology, Rutgers University, 123 Bevier Rd, Piscataway, NJ 08854, USA., Yelick PC; Division of Craniofacial and Molecular Genetics, Department of Orthodontics, Tufts University School of Dental Medicine, 1 Kneeland Avenue, Boston, MA 02111, USA. |
| المصدر: | Bioengineering (Basel, Switzerland) [Bioengineering (Basel)] 2022 May 17; Vol. 9 (5). Date of Electronic Publication: 2022 May 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101676056 Publication Model: Electronic Cited Medium: Print ISSN: 2306-5354 (Print) Linking ISSN: 23065354 NLM ISO Abbreviation: Bioengineering (Basel) Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, [2014]- |
| مستخلص: | Here, we describe the characterization of tooth-germ organoids, three-dimensional (3D) constructs cultured in vitro with the potential to develop into living teeth. To date, the methods used to successfully create tooth organoids capable of forming functional teeth have been quite limited. Recently, hydrogel microparticles (HMP) have demonstrated utility in tissue repair and regeneration based on their useful characteristics, including their scaffolding ability, effective cell and drug delivery, their ability to mimic the natural tissue extracellular matrix, and their injectability. These outstanding properties led us to investigate the utility of using HMPs (average diameter: 158 ± 32 µm) derived from methacrylated gelatin (GelMA) (degree of substitution: 100%) to create tooth organoids. The tooth organoids were created by seeding human dental pulp stem cells (hDPSCs) and porcine dental epithelial cells (pDE) onto the HMPs, which provided an extensive surface area for the cells to effectively attach and proliferate. Interestingly, the cell-seeded HMPs cultured on low-attachment tissue culture plates with gentle rocking self-assembled into organoids, within which the cells maintained their viability and morphology throughout the incubation period. The self-assembled organoids reached a volume of ~50 mm 3 within two weeks of the in vitro tissue culture. The co-cultured hDPSC-HMP and pDE-HMP structures effectively attached to each other without any externally applied forces. The presence of polarized, differentiated dental cells in these composite tooth-bud organoids demonstrated the potential of self-assembled dental cell HMPs to form tooth-bud organoid-like structures for potential applications in tooth regeneration strategies. |
| References: | J Oral Maxillofac Surg. 2009 Feb;67(2):335-47. (PMID: 19138608) Dev Dyn. 2019 Jan;248(1):129-139. (PMID: 30106495) Biomaterials. 2015 Dec;73:254-71. (PMID: 26414409) Biomater Sci. 2019 Dec 17;8(1):438-449. (PMID: 31746842) Trends Biotechnol. 2019 Sep;37(9):1011-1028. (PMID: 30902347) Polymers (Basel). 2020 Oct 19;12(10):. (PMID: 33086577) Acta Biomater. 2016 Apr 15;35:57-67. (PMID: 26931056) Materials (Basel). 2019 Apr 02;12(7):. (PMID: 30986948) Sci Rep. 2017 Mar 16;7:44522. (PMID: 28300208) Adv Funct Mater. 2017 Dec 8;27(46):. (PMID: 29755309) Acta Biomater. 2020 Sep 1;113:305-316. (PMID: 32663663) J Dent Res. 2017 Feb;96(2):192-199. (PMID: 28106508) Methods Mol Biol. 2019;1922:139-150. (PMID: 30838572) J Dent Res. 2013 Apr;92(4):329-34. (PMID: 23458883) Mater Sci Eng C Mater Biol Appl. 2021 Feb;121:111850. (PMID: 33579484) Acta Biomater. 2021 Apr 1;124:315-326. (PMID: 33465507) Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13475-80. (PMID: 19666587) Sci Rep. 2016 Aug 09;6:31036. (PMID: 27503340) Sci Rep. 2019 May 3;9(1):6863. (PMID: 31053756) J Tissue Eng Regen Med. 2017 Dec;11(12):3326-3336. (PMID: 28066993) Cell Prolif. 2018 Aug;51(4):e12479. (PMID: 30028040) Biomaterials. 2002 Jul;23(13):2717-22. (PMID: 12059021) Methods. 2009 Feb;47(2):122-8. (PMID: 18845257) J Dent Res. 2004 Jul;83(7):518-22. (PMID: 15218039) Nat Rev Mater. 2020 Jan;5(1):20-43. (PMID: 34123409) Int J Biol Macromol. 2019 Feb 1;122:1152-1162. (PMID: 30218727) Int J Biol Macromol. 2019 Apr 15;127:340-348. (PMID: 30658141) J Dent Res. 2018 Sep;97(10):1144-1151. (PMID: 29879370) J Cell Sci. 2003 May 1;116(Pt 9):1647-8. (PMID: 12665545) J Tissue Eng Regen Med. 2018 Apr;12(4):e1899-e1910. (PMID: 29193831) J Tissue Eng Regen Med. 2018 Jan;12(1):204-216. (PMID: 28079309) Nanoscale. 2017 Dec 21;10(1):222-230. (PMID: 29210437) Nat Methods. 2007 Mar;4(3):227-30. (PMID: 17322892) Ann Biomed Eng. 2016 Jun;44(6):1946-58. (PMID: 27160672) PLoS One. 2011;6(7):e21531. (PMID: 21765896) J Orthop Translat. 2015 Mar 13;3(2):51-57. (PMID: 30035040) J Biomed Mater Res A. 2017 Sep;105(9):2597-2607. (PMID: 28544201) Lab Chip. 2020 Feb 21;20(4):760-777. (PMID: 31951241) J Dent Res. 2002 Oct;81(10):695-700. (PMID: 12351668) Biofabrication. 2020 Jul 01;12(3):035029. (PMID: 32428889) J Biomed Mater Res A. 2011 Jun 15;97(4):414-22. (PMID: 21484985) J Dent Res. 2017 May;96(5):516-523. (PMID: 28118552) J Biomater Sci Polym Ed. 2018 Oct;29(14):1764-1784. (PMID: 29999478) Chem Rev. 2001 Jul;101(7):1869-79. (PMID: 11710233) |
| معلومات مُعتمدة: | R01 DE026731 United States DE NIDCR NIH HHS; W81-XWH-14-2-0004 W81-XWH-14-2-0004; CF-04 AFIRM II; T32EB005583 United States NH NIH HHS |
| فهرسة مساهمة: | Keywords: GelMA microparticles; human dental pulp stem cells (hDPSCs); hydrogel microparticles (HMPs); porcine dental endothelial cells (pDE); self-assembled organoid structures; tooth-bud organoids |
| تواريخ الأحداث: | Date Created: 20220527 Latest Revision: 20220716 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC9137977 |
| DOI: | 10.3390/bioengineering9050215 |
| PMID: | 35621493 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!