دورية أكاديمية
Molecular characterization of drug-resistant Mycobacterium tuberculosis among Filipino patients derived from the national tuberculosis prevalence survey Philippines 2016.
| العنوان: | Molecular characterization of drug-resistant Mycobacterium tuberculosis among Filipino patients derived from the national tuberculosis prevalence survey Philippines 2016. |
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| المؤلفون: | Montoya JC; College of Medicine, University of the Philippines Manila, Pedro Gil St., Ermita, Manila, Philippines. Electronic address: jmontoya204@gmail.com., Malabad JCM; College of Medicine, University of the Philippines Manila, Pedro Gil St., Ermita, Manila, Philippines. Electronic address: jmmalabad@up.edu.ph., Ang CF; College of Medicine, University of the Philippines Manila, Pedro Gil St., Ermita, Manila, Philippines., Reyes LT; National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, 9002, Research Drive, Filinvest Corporate City, Alabang, Muntinlupa City, Metro Manila, Philippines., Basilio RP; National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, 9002, Research Drive, Filinvest Corporate City, Alabang, Muntinlupa City, Metro Manila, Philippines., Lim DR; National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, 9002, Research Drive, Filinvest Corporate City, Alabang, Muntinlupa City, Metro Manila, Philippines., Amarillo MLE; College of Medicine, University of the Philippines Manila, Pedro Gil St., Ermita, Manila, Philippines., Ama MCG; National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, 9002, Research Drive, Filinvest Corporate City, Alabang, Muntinlupa City, Metro Manila, Philippines., Phelan JE; Department of Infection Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK., Hibberd ML; Department of Infection Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK., Clark TG; Department of Infection Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK. |
| المصدر: | Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2022 Jul; Vol. 135, pp. 102211. Date of Electronic Publication: 2022 May 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Edinburgh, New York : Churchill Livingstone, c2001- |
| مواضيع طبية MeSH: | Mycobacterium tuberculosis* , Tuberculosis*/microbiology , Tuberculosis, Multidrug-Resistant*/diagnosis , Tuberculosis, Multidrug-Resistant*/drug therapy , Tuberculosis, Multidrug-Resistant*/epidemiology, Antitubercular Agents/pharmacology ; Antitubercular Agents/therapeutic use ; Drug Resistance, Multiple, Bacterial/genetics ; Genotype ; Humans ; Microbial Sensitivity Tests ; Philippines/epidemiology ; Phylogeny ; Prevalence |
| مستخلص: | Tuberculosis, caused by Mycobacterium tuberculosis, remains a high burden disease and leading cause of mortality in the Philippines. Understanding the genetic diversity of M. tuberculosis strains in the population, including those that are multi-drug resistant (MDR), will aid in formulating strategies for effective TB control and prevention. By whole genome sequencing of M. tuberculosis isolates (n = 100) from patients of the Philippine 2016 National Tuberculosis Prevalence Survey, we sought to provide a baseline assessment of the genotypic and phylogenetic characteristics of the isolates. The majority (96/100) of the isolates were EAI2-Manila strain-type (lineage 1), with one Lineage 2 (Beijing), one Lineage 3 (CAS1), and two Lineage 4 (LAM9) strains. The EAI2-Manila clade was not significantly associated with patient's phenotypic and in silico drug resistance profile. Five (5/6) MDR-TB isolates predicted by in silico profiling were concordant with phenotypic drug resistance profile. Twenty-one mutations were identified in nine drug resistance-related genes, all of which have been reported in previous studies. Overall, the results from this study contribute to the growing data on the molecular characteristics of Philippine M. tuberculosis isolates, which can help in developing tools for rapid diagnosis of TB in the country, and thereby reducing the high burden of disease. (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| معلومات مُعتمدة: | MR/M01360X/1 United Kingdom MRC_ Medical Research Council; MR/N010469/1 United Kingdom MRC_ Medical Research Council; MR/R025576/1 United Kingdom MRC_ Medical Research Council; MR/R020973/1 United Kingdom MRC_ Medical Research Council; BB/R013063/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
| فهرسة مساهمة: | Keywords: EAI2-Manila; M. tuberculosis; Molecular epidemiology |
| المشرفين على المادة: | 0 (Antitubercular Agents) |
| تواريخ الأحداث: | Date Created: 20220531 Date Completed: 20220810 Latest Revision: 20220829 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.tube.2022.102211 |
| PMID: | 35636102 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1873-281X |
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| DOI: | 10.1016/j.tube.2022.102211 |