دورية أكاديمية
أعرض في EDS

Mettl3 downregulation in germinal vesicle oocytes inhibits mRNA decay and the first polar body extrusion during maturation†.

التفاصيل البيبلوغرافية
العنوان: Mettl3 downregulation in germinal vesicle oocytes inhibits mRNA decay and the first polar body extrusion during maturation†.
المؤلفون: Zhu Y; Medical Experiment Center, Guangdong Second Provincial General Hospital, Guangdong, PR China., Wu W; Medical Experiment Center, Guangdong Second Provincial General Hospital, Guangdong, PR China., Chen S; Center for Reproductive Medicine, Guangdong Second Provincial General Hospital, Guangdong, PR China., Zhang Z; Medical Experiment Center, Guangdong Second Provincial General Hospital, Guangdong, PR China., Zhang G; Center for Reproductive Medicine, Guangdong Second Provincial General Hospital, Guangdong, PR China., Li J; Medical Experiment Center, Guangdong Second Provincial General Hospital, Guangdong, PR China., Jiang M; Center for Reproductive Medicine, Guangdong Second Provincial General Hospital, Guangdong, PR China.
المصدر: Biology of reproduction [Biol Reprod] 2022 Sep 12; Vol. 107 (3), pp. 765-778.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: United States NLM ID: 0207224 Publication Model: Print Cited Medium: Internet ISSN: 1529-7268 (Electronic) Linking ISSN: 00063363 NLM ISO Abbreviation: Biol Reprod Subsets: MEDLINE
أسماء مطبوعة: Publication: 2017- : New York : Oxford University Press
Original Publication: Champaign, Ill. : Society for the Study of Reproduction
مواضيع طبية MeSH: Methyltransferases*/genetics , Methyltransferases*/metabolism , Oocytes*/metabolism , Polar Bodies*, Adenosine/metabolism ; Animals ; Down-Regulation ; Mice ; RNA Stability ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism
مستخلص: In oocytes, mRNA decay is essential for maturation and subsequent events, such as maternal-zygotic transition, zygotic genomic activation, and embryo development. Reversible N6-methyladenosine RNA methylation directly regulates transcription, pre-mRNA splicing, mRNA export, mRNA stability, and translation. Here, we identified that downregulation of N6-methyladenosine modification by microinjecting a methyltransferase-like 3 (Mettl3)-specific small interfering RNA into mouse germinal vesicle oocytes led to defects in meiotic spindles and the first polar body extrusion during maturation in vitro. By further quantitative real-time polymerase chain reaction and Poly(A)-tail assay analysis, we found that N6-methyladenosine methylation mainly acts by reducing deadenylation of mRNAs mediated by the carbon catabolite repression 4-negative on TATA less system, thereby causing mRNA accumulation in oocytes. Meanwhile, transcriptome analysis of germinal vesicle oocytes revealed the downregulation of transcripts of several genes encoding ribosomal subunits proteins in the Mettl3 small interfering RNA-treated group, suggesting that N6-methyladenosine modification might affect translation. Together, our results indicate that RNA methylation accelerates mRNA decay, confirming the critical role of RNA clearance in oocyte maturation.
(© The Author(s) 2022. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
فهرسة مساهمة: Keywords: Mettl3; deadenylation; m6A modification; mRNA decay; oocyte maturation
المشرفين على المادة: 0 (RNA, Messenger)
0 (RNA, Small Interfering)
EC 2.1.1.- (Methyltransferases)
EC 2.1.1.- (Mettl3 protein, mouse)
K72T3FS567 (Adenosine)
تواريخ الأحداث: Date Created: 20220531 Date Completed: 20220916 Latest Revision: 20220926
رمز التحديث: 20221213
DOI: 10.1093/biolre/ioac112
PMID: 35639638
قاعدة البيانات: MEDLINE
الوصف
تدمد:1529-7268
DOI:10.1093/biolre/ioac112