دورية أكاديمية
Metabolite-Based Mutualistic Interaction between Two Novel Clostridial Species from Pit Mud Enhances Butyrate and Caproate Production.
| العنوان: | Metabolite-Based Mutualistic Interaction between Two Novel Clostridial Species from Pit Mud Enhances Butyrate and Caproate Production. |
|---|---|
| المؤلفون: | Sun H; Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China.; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China., Chai LJ; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China.; Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China., Fang GY; Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China.; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China., Lu ZM; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China.; Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China., Zhang XJ; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China.; Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China., Wang ST; National Engineering Research Center of Solid-State Brewing, Luzhou, People's Republic of China., Shen CH; National Engineering Research Center of Solid-State Brewing, Luzhou, People's Republic of China., Shi JS; School of Life Science and Health Engineering, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China., Xu ZH; Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China.; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan Universitygrid.258151.a, Wuxi, People's Republic of China.; National Engineering Research Center of Solid-State Brewing, Luzhou, People's Republic of China. |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2022 Jul 12; Vol. 88 (13), pp. e0048422. Date of Electronic Publication: 2022 Jun 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Butyrates* , Caproates*/metabolism, Alcoholic Beverages/microbiology ; Clostridium/genetics ; Clostridium/metabolism ; Fermentation |
| مستخلص: | Pit mud microbial consortia play crucial roles in the formation of Chinese strong-flavor baijiu's key flavor-active compounds, especially butyric and caproic acids. Clostridia , one of the abundant bacterial groups in pit mud, were recognized as important butyric and caproic acid producers. Research on the interactions of the pit mud microbial community mainly depends on correlation analysis at present. Interaction between Clostridium and other microorganisms and its involvement in short/medium-chain fatty acid (S/MCFA) metabolism are still unclear. We previously found coculture of two clostridial strains isolated from pit mud, Clostridium fermenticellae JN500901 ( C. 901) and Novisyntrophococcus fermenticellae JN500902 ( N. 902), could enhance S/MCFA accumulation. Here, we investigated their underlying interaction mechanism through the combined analysis of phenotype, genome, and transcriptome. Compared to monocultures, coculture of C. 901 and N. 902 obviously promoted their growth, including shortening the growth lag phase and increasing biomass, and the accumulation of butyric acid and caproic acid. The slight effects of inoculation ratio and continuous passage on the growth and metabolism of coculture indicated the relative stability of their interaction. Transwell coculture and transcriptome analysis showed the interaction between C. 901 and N. 902 was accomplished by metabolite exchange, i.e., formic acid produced by C. 901 activated the Wood-Ljungdahl pathway of N. 902, thereby enhancing its production of acetic acid, which was further converted to butyric acid and caproic acid by C. 901 through reverse β-oxidation. This work demonstrates the potential roles of mutually beneficial interspecies interactions in the accumulation of key flavor compounds in pit mud. IMPORTANCE Microbial interactions played crucial roles in influencing the assembly, stability, and function of the microbial community. The metabolites of pit mud microbiota are the key to flavor formation of Chinese strong-flavor baijiu. So far, researches on the interactions of the pit mud microbial community have been mainly based on the correlation analysis of sequencing data, and more work needs to be performed to unveil the complicated interaction patterns. Here, we identified a material exchange-based mutualistic interaction system involving two fatty acid-producing clostridial strains ( Clostridium fermenticellae JN500901 and Novisyntrophococcus fermenticellae JN500902) isolated from pit mud and systematically elucidated their interaction mechanism for promoting the production of butyric acid and caproic acid, the key flavor-active compounds of baijiu. Our findings provide a new perspective for understanding the complicated interactions of pit mud microorganisms. |
| فهرسة مساهمة: | Keywords: Chinese strong-flavor baijiu; Clostridium coculture; microbial interaction; pit mud; transcriptome |
| المشرفين على المادة: | 0 (Butyrates) 0 (Caproates) 1F8SN134MX (hexanoic acid) |
| تواريخ الأحداث: | Date Created: 20220613 Date Completed: 20220725 Latest Revision: 20221214 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9275218 |
| DOI: | 10.1128/aem.00484-22 |
| PMID: | 35695571 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5336 |
|---|---|
| DOI: | 10.1128/aem.00484-22 |