دورية أكاديمية
أعرض في EDS

The effect of cold exposure on circulating transcript levels of immune genes in Dutch South Asian and Dutch Europid men.

التفاصيل البيبلوغرافية
العنوان: The effect of cold exposure on circulating transcript levels of immune genes in Dutch South Asian and Dutch Europid men.
المؤلفون: Straat ME; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: M.E.Straat@lumc.nl., Martinez-Tellez B; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands., Janssen LGM; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands., van Veen S; Department of Infectious Diseases, Leiden University Medical Center, Leiden, the Netherlands., van Eenige R; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands., Kharagjitsing AV; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands; Department of Diabetes and Endocrinology, University Hospital Brussels and Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium., van den Berg SAA; Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands; Department of Internal Medicine, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands., de Rijke YB; Department of Clinical Chemistry, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands., Haks MC; Department of Infectious Diseases, Leiden University Medical Center, Leiden, the Netherlands., Rensen PCN; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands., Boon MR; Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, the Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.
المصدر: Journal of thermal biology [J Therm Biol] 2022 Jul; Vol. 107, pp. 103259. Date of Electronic Publication: 2022 May 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 7600115 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0306-4565 (Print) Linking ISSN: 03064565 NLM ISO Abbreviation: J Therm Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, New York, Pergamon Press.
مواضيع طبية MeSH: Asian People*/genetics , Fasting*, Adaptor Proteins, Signal Transducing ; Adult ; Humans ; Male ; RNA, Messenger/genetics ; Young Adult
مستخلص: Objectives: Although cold exposure is commonly believed to be causally related to acute viral respiratory infections, its effect on the immune system is largely unexplored. In this study, we determined transcript levels of a large panel of immune genes in blood before and after cold exposure. We included both Dutch Europid and Dutch South Asian men to address whether the immune system is differently regulated in the metabolically vulnerable South Asian population.
Methods: Fasted blood samples were obtained from nonobese Dutch Europid (n = 11; mean age 26 ± 3 y) and Dutch South Asian (n = 12; mean age 28 ± 3 y) men before and directly after short-term (∼2.5 h) mild cold exposure. Transcript levels of 144 immune genes were measured using a dual-color reverse transcriptase multiplex ligation-dependent probe amplification (dcRT-MLPA) assay.
Results: Cold exposure acutely upregulated mRNA levels of GNLY (+35%, P < 0.001) and PRF1 (+45%, P < 0.001), which encode cytotoxic proteins, and CCL4 (+8%, P < 0.01) and CCL5 (+5%, P < 0.05), both pro-inflammatory chemokines. At thermoneutrality, mRNA levels of four markers of the nucleotide-binding oligomerization domain (NOD)-like receptor (NLR)-family, involved in inflammasomes, were lower in Dutch South Asians compared to Dutch Europids, namely NLRP2 (-57%, P < 0.05), NLRP7 (-17%, P < 0.05), NLRP10 (-21%, P < 0.05), and NLRC4 (-23%, P < 0.05).
Conclusions: Mild cold exposure acutely increases mRNA levels of genes involved in cytotoxicity of immune cells in blood. In addition, Dutch South Asians display lower circulating mRNA levels of inflammasome genes compared to Dutch Europids.
(Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
فهرسة مساهمة: Keywords: Cold stimulus; Ethnicity; Gene expression; Immune system; Metabolic syndrome
المشرفين على المادة: 0 (Adaptor Proteins, Signal Transducing)
0 (NLRP7 protein, human)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20220614 Date Completed: 20220616 Latest Revision: 20221207
رمز التحديث: 20231215
DOI: 10.1016/j.jtherbio.2022.103259
PMID: 35701026
قاعدة البيانات: MEDLINE
الوصف
تدمد:0306-4565
DOI:10.1016/j.jtherbio.2022.103259