تقرير
Report of a clinical and laboratory management of cell therapy for knee cartilage in the face of mycoplasma contamination.
| العنوان: | Report of a clinical and laboratory management of cell therapy for knee cartilage in the face of mycoplasma contamination. |
|---|---|
| المؤلفون: | Zorzi AR; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Antonioli E; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Godoy JAP; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Okamoto OK; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Kondo AT; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Kutner JM; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Kaleka CC; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Cohen M; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Ferretti M; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil. |
| المصدر: | Einstein (Sao Paulo, Brazil) [Einstein (Sao Paulo)] 2022 Jun 17; Vol. 20, pp. eRC6918. Date of Electronic Publication: 2022 Jun 17 (Print Publication: 2022). |
| نوع المنشور: | Case Reports |
| اللغة: | English |
| بيانات الدورية: | Publisher: Instituto de Ensino e Pesquisa Albert Einstein Country of Publication: Brazil NLM ID: 101281800 Publication Model: eCollection Cited Medium: Internet ISSN: 2317-6385 (Electronic) Linking ISSN: 16794508 NLM ISO Abbreviation: Einstein (Sao Paulo) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: São Paulo, SP : Instituto de Ensino e Pesquisa Albert Einstein |
| مواضيع طبية MeSH: | Cartilage, Articular*/injuries , Mycoplasma*, Cell- and Tissue-Based Therapy ; Chondrocytes ; Humans ; Knee Joint ; Male ; Middle Aged |
| مستخلص: | To describe a case of autologous chondrocyte implantation after cell culture contamination by Mycoplasma pneumoniae and the measures taken to successfully complete cell therapy in a patient with focal chondral lesion. A 45-year-old male patient, complaining of chronic pain on the knee and no history of trauma. He had a chondral lesion in the trochlear region of the femur and clinical tests compatible with pain in the anterior compartment of the knee. Conservative treatment failed to alleviate symptoms. Surgical treatment was indicated, but due to the size of the lesion, membrane-assisted autologous chondrocyte implantation was the technique of choice. Cartilage biopsies were collected from the intercondylar region of the distal femur. After isolation, chondrocytes were expanded ex vivo in a trained laboratory, for three weeks, and seeded onto a commercially available collagen membrane prior to implantation in the knee. Two days before surgery, a cell culture sample tested positive for Mycoplasma pneumoniae. The source of contamination was found to be autologous blood serum, extracted from the patient´s peripheral vein, and used to supplement the cell culture medium. After treating the patient with antibiotics, all procedures were repeated and the new final cell product, free from contaminants, was successfully implanted. We discuss the strategies available to deal with this situation, and describe the results of this particular case, which led to modifications in the autologous chondrocyte implant protocol. |
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| تواريخ الأحداث: | Date Created: 20220622 Date Completed: 20220623 Latest Revision: 20220716 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC9239536 |
| DOI: | 10.31744/einstein_journal/2022RC6918 |
| PMID: | 35730808 |
| قاعدة البيانات: | MEDLINE |
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