دورية أكاديمية
Tracking the Molecular Scenarios for Tumorigenic Remodeling of Extracellular Matrix Based on Gene Expression Profiling in Equine Skin Neoplasia Models.
| العنوان: | Tracking the Molecular Scenarios for Tumorigenic Remodeling of Extracellular Matrix Based on Gene Expression Profiling in Equine Skin Neoplasia Models. |
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| المؤلفون: | Podstawski P; Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, Balice, 32-083 Kraków, Poland.; Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza 24/28, 30-059 Kraków, Poland., Ropka-Molik K; Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, Balice, 32-083 Kraków, Poland., Semik-Gurgul E; Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, Balice, 32-083 Kraków, Poland., Samiec M; Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, Krakowska 1 Street, Balice, 32-083 Kraków, Poland., Skrzyszowska M; Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, Krakowska 1 Street, Balice, 32-083 Kraków, Poland., Podstawski Z; Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza 24/28, 30-059 Kraków, Poland., Szmatoła T; Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, Balice, 32-083 Kraków, Poland.; Center for Experimental and Innovative Medicine, University of Agriculture in Krakow, Rędzina 1c Street, 30-248 Kraków, Poland., Witkowski M; Institute of Veterinary Medicine, University Centre of Veterinary Medicine JU-AU, Mickiewicza 24/28, 30-059 Kraków, Poland.; Horse Clinic Służewiec, Puławska 266 Street, 02-684 Warsaw, Poland., Pawlina-Tyszko K; Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, Balice, 32-083 Kraków, Poland. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2022 Jun 10; Vol. 23 (12). Date of Electronic Publication: 2022 Jun 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Bovine papillomavirus 1*/genetics , Horse Diseases*/metabolism , Papillomavirus Infections* , Sarcoidosis* , Skin Diseases* , Skin Neoplasms*/genetics , Skin Neoplasms*/metabolism , Skin Neoplasms*/veterinary, Animals ; Cell Transformation, Neoplastic ; Extracellular Matrix/metabolism ; Gene Expression Profiling ; Horses/genetics |
| مستخلص: | An important component of tissues is the extracellular matrix (ECM), which not only forms a tissue scaffold, but also provides the environment for numerous biochemical reactions. Its composition is strictly regulated, and any irregularities can result in the development of many diseases, including cancer. Sarcoid is the most common skin cancer in equids. Its formation results from the presence of the genetic material of the bovine papillomavirus (BPV). In addition, it is assumed that sarcoid-dependent oncogenic transformation arises from a disturbed wound healing process, which may be due to the incorrect functioning of the ECM. Moreover, sarcoid is characterized by a failure to metastasize. Therefore, in this study we decided to investigate the differences in the expression profiles of genes related not only to ECM remodeling, but also to the cell adhesion pathway, in order to estimate the influence of disturbances within the ECM on the sarcoid formation process. Furthermore, we conducted comparative research not only between equine sarcoid tissue bioptates and healthy skin-derived explants, but also between dermal fibroblast cell lines transfected and non-transfected with a construct encoding the E4 protein of the BP virus, in order to determine its effect on ECM disorders. The obtained results strongly support the hypothesis that ECM-related genes are correlated with sarcoid formation. The deregulated expression of selected genes was shown in both equine sarcoid tissue bioptates and adult cutaneous fibroblast cell (ACFC) lines neoplastically transformed by nucleofection with gene constructs encoding BPV1-E1^E4 protein. The identified genes ( CD99 , ITGB1 , JAM3 and CADM1 ) were up- or down-regulated, which pinpointed the phenotypic differences from the backgrounds noticed for adequate expression profiles in other cancerous or noncancerous tumors as reported in the available literature data. Unravelling the molecular pathways of ECM remodeling and cell adhesion in the in vivo and ex vivo models of epidermal/dermal sarcoid-related cancerogenesis might provide powerful tools for further investigations of genetic and epigenetic biomarkers for both silencing and re-initiating the processes of sarcoid-dependent neoplasia. Recognizing those biomarkers might insightfully explain the relatively high capacity of sarcoid-descended cancerous cell derivatives to epigenomically reprogram their nonmalignant neoplastic status in domestic horse cloned embryos produced by somatic cell nuclear transfer (SCNT). |
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| معلومات مُعتمدة: | DI2016 012746 Ministry of Science and Higher Education; 04-19-11-21 National Research Institute of Animal Production |
| فهرسة مساهمة: | Keywords: ECM remodeling; RNA-seq; cell adhesion; dermal tissue; domestic horse; molecular pathway; procancerous tumorigenesis; sarcoid |
| تواريخ الأحداث: | Date Created: 20220624 Date Completed: 20220627 Latest Revision: 20220716 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9223705 |
| DOI: | 10.3390/ijms23126506 |
| PMID: | 35742950 |
| قاعدة البيانات: | MEDLINE |
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