دورية أكاديمية
أعرض في EDS

Possible Underlying Mechanisms for the Renoprotective Effect of Retinoic Acid-Pretreated Wharton's Jelly Mesenchymal Stem Cells against Renal Ischemia/Reperfusion Injury.

التفاصيل البيبلوغرافية
العنوان: Possible Underlying Mechanisms for the Renoprotective Effect of Retinoic Acid-Pretreated Wharton's Jelly Mesenchymal Stem Cells against Renal Ischemia/Reperfusion Injury.
المؤلفون: Barakat M; Department of Biochemistry, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.; Institute of Global Public Health and Human Ecology, School of Science and Engineering, American University, Cairo 11835, Egypt., Hussein AM; Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt., Salama MF; Department of Biochemistry, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Awadalla A; Urology and Nephrology Center, Mansoura University, Mansoura 35516, Egypt., Barakat N; Urology and Nephrology Center, Mansoura University, Mansoura 35516, Egypt., Serria M; Department of Biochemistry, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt., El-Shafey M; Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.; Physiological Sciences Department, Fakeeh College for Medical Sciences, Jeddah 21461, Saudi Arabia., El-Sherbiny M; Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh 71666, Saudi Arabia., El Adl MA; Department of Biochemistry, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
المصدر: Cells [Cells] 2022 Jun 22; Vol. 11 (13). Date of Electronic Publication: 2022 Jun 22.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Mesenchymal Stem Cell Transplantation*/methods , Mesenchymal Stem Cells*/metabolism , Reperfusion Injury*/metabolism , Wharton Jelly*, Animals ; Creatinine/metabolism ; Cytokines/metabolism ; Interleukin-6/metabolism ; Ischemia/metabolism ; Kidney/metabolism ; Male ; NF-kappa B/metabolism ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase/metabolism ; Tretinoin/metabolism ; Tretinoin/pharmacology ; bcl-2-Associated X Protein/metabolism ; beta Catenin/metabolism
مستخلص: Objectives: The current work investigated the effect of Wharton jelly mesenchymal stem cells (WJ-MSCs) pretreated with all-trans-retinoic acid (ATRA) on renal ischemia in rats and the possible role of oxidative stress, apoptotic and Wnt/β-Catenin signaling pathways, and inflammatory cytokines in their effects. Methods: The study included 90 male Sprague Dawley rats that were allocated to five groups (n = 18 rats): (I) Sham-operated group (right nephrectomy was performed); (II) Ischemia/reperfusion injury (IRI) group, a sham group with 45-min renal ischemia on the left kidney; (III) ATRA group, an ischemic group with an intravenous (i.v.) administration of ATRA 10 µM, 10 min post-surgery); (IV) WJ-MSCs group, an IRI group with an i.v. administration of 150 µL containing 7 × 106 WJ-MSCs, 10 min post-surgery; (V) WJ-MSCs + ATRA group, an IRI group with an i.v. administration of 150 µL of 7 × 106 WJ-MSCs pretreated with 10 µM ATRA. At the end of the experiments, serum creatinine, BUN micro-albuminuria (MAU), urinary protein, markers of redox state in the left kidney (MDA, CAT, SOD, and GSH), and the expression of Bax, IL-6, HIF-1α, Wnt7B, and β-catenin genes at the level of mRNA as well as for immunohistochemistry for NFkB and β-Catenin markers were analyzed. Results: The current study found that 45-min of renal ischemia resulted in significant impairment of kidney function (evidenced by the increase in serum creatinine, BUN, and urinary proteins) and deterioration of the kidney morphology, which was associated with a significant increase in redox state (evidenced by an increase in MDA and a decrease in GSH, SOD, and CAT), and a significant increase in inflammatory and apoptotic processes (evidenced by an increase in Bax and IL-6, NFkB, Wnt7B, β-catenin and HIF-1α) in kidney tissues (p < 0.05). On the other hand, treatment with ATRA, WJ-MSCs, or a combination of both, caused significant improvement in kidney function and morphology, which was associated with significant attenuation of oxidative stress, apoptotic markers, and inflammatory cytokines (IL6 and NFkB) with the upregulation of HIF-1α and β-catenin in kidney tissues (p < 0.05). Moreover, the renoprotective effect of WJ-MSCs pretreated with ATRA was more potent than WJ-MSCs alone. Conclusions: It is concluded that preconditioning of WJ-MSCs with ATRA may enhance their renoprotective effect. This effect could be due to the upregulation of the beta-catenin/Wnt pathway and attenuation of apoptosis, inflammation, and oxidative stress.
References: Pediatr Nephrol. 2015 Apr;30(4):561-6. (PMID: 24777535)
Int J Mol Sci. 2013 May 31;14(6):11692-712. (PMID: 23727936)
Biomed Pharmacother. 2021 Aug;140:111686. (PMID: 34015581)
PLoS One. 2015 Jul 17;10(7):e0133414. (PMID: 26186635)
Int J Urol. 2020 Nov;27(11):1039-1049. (PMID: 32794300)
Nephrol Dial Transplant. 2004 Oct;19(10):2464-71. (PMID: 15316095)
Can J Physiol Pharmacol. 2016 Sep;94(9):936-46. (PMID: 27411029)
Front Chem. 2020 Aug 21;8:732. (PMID: 32974285)
Acta Biochim Biophys Sin (Shanghai). 2017 Apr 1;49(4):338-348. (PMID: 28338909)
Stem Cells Transl Med. 2016 Aug;5(8):1048-57. (PMID: 27280799)
Avicenna J Med Biotechnol. 2015 Jul-Sep;7(3):106-12. (PMID: 26306150)
Am J Physiol Renal Physiol. 2010 Jan;298(1):F158-66. (PMID: 19864300)
World J Stem Cells. 2019 Aug 26;11(8):548-564. (PMID: 31523373)
Stem Cells Int. 2019 Apr 9;2019:9628536. (PMID: 31093291)
Front Med (Lausanne). 2020 Jun 02;7:201. (PMID: 32582723)
Cell Immunol. 2012;274(1-2):46-53. (PMID: 22414555)
Cells. 2019 Feb 28;8(3):. (PMID: 30823476)
J Renal Inj Prev. 2015 Jun 01;4(2):20-7. (PMID: 26060833)
Front Immunol. 2019 Apr 16;10:815. (PMID: 31040851)
Front Med (Lausanne). 2015 Mar 23;2:16. (PMID: 25853135)
Cell Physiol Biochem. 2017;44(5):1980-1994. (PMID: 29237155)
Diabetes. 2005 Aug;54(8):2424-9. (PMID: 16046310)
Kidney Blood Press Res. 2020;45(1):95-108. (PMID: 31927554)
Sci Rep. 2017 Aug 16;7(1):8484. (PMID: 28814814)
J Am Soc Nephrol. 2016 Sep;27(9):2658-69. (PMID: 26823548)
Exp Ther Med. 2020 Dec;20(6):145. (PMID: 33093883)
PLoS One. 2015 Jan 05;10(1):e115947. (PMID: 25559736)
Annu Rev Med. 2016;67:293-307. (PMID: 26768243)
Am J Physiol Gastrointest Liver Physiol. 2003 Sep;285(3):G621-9. (PMID: 12773301)
Exp Ther Med. 2018 Sep;16(3):2008-2012. (PMID: 30186432)
Metab Brain Dis. 2017 Feb;32(1):185-193. (PMID: 27549229)
Stroke. 2003 Mar;34(3):671-5. (PMID: 12624290)
PPAR Res. 2007;2007:87934. (PMID: 17846663)
Stem Cells Int. 2020 Dec 17;2020:8857457. (PMID: 33381188)
Front Immunol. 2016 Sep 22;7:378. (PMID: 27713747)
Am J Stem Cells. 2016 May 15;5(1):1-10. (PMID: 27335697)
Kidney Int. 2012 Sep;82(5):509-11. (PMID: 22892856)
Stem Cell Res Ther. 2016 Apr 30;7(1):68. (PMID: 27137761)
Gen Physiol Biophys. 2018 Mar;37(2):193-204. (PMID: 29593125)
Nat Commun. 2020 Aug 26;11(1):4265. (PMID: 32848154)
Eur Urol Open Sci. 2020 Aug 17;20:48-53. (PMID: 34337457)
Cytotherapy. 2014 May;16(5):579-85. (PMID: 24113425)
Stroke. 2000 Jul;31(7):1715-20. (PMID: 10884478)
Kidney Int. 2002 Nov;62(5):1539-49. (PMID: 12371954)
J Food Biochem. 2021 Feb;45(2):e13628. (PMID: 33502024)
Front Neurosci. 2021 Feb 25;15:628983. (PMID: 33716653)
J Vet Intern Med. 2019 May;33(3):1353-1361. (PMID: 30924554)
J Recept Signal Transduct Res. 2021 Feb;41(1):15-18. (PMID: 32580617)
Environ Sci Pollut Res Int. 2017 Aug;24(24):19980-19989. (PMID: 28691127)
PLoS One. 2013 Nov 21;8(11):e79432. (PMID: 24278135)
Mol Cell Biochem. 1995 Jun 7-21;147(1-2):5-12. (PMID: 7494554)
Cell Death Discov. 2015 Aug 24;1:15007. (PMID: 27551443)
Cell Transplant. 2011;20(8):1193-208. (PMID: 21092414)
فهرسة مساهمة: Keywords: WJ-MSCs; Wnt/β-catenin; renal ischemia; retinoic acid
المشرفين على المادة: 0 (Cytokines)
0 (Interleukin-6)
0 (NF-kappa B)
0 (bcl-2-Associated X Protein)
0 (beta Catenin)
5688UTC01R (Tretinoin)
AYI8EX34EU (Creatinine)
EC 1.15.1.1 (Superoxide Dismutase)
تواريخ الأحداث: Date Created: 20220709 Date Completed: 20220712 Latest Revision: 20230308
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9266019
DOI: 10.3390/cells11131997
PMID: 35805083
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4409
DOI:10.3390/cells11131997