دورية أكاديمية
أعرض في EDS

Do Extracellular Vesicles Derived from Mesenchymal Stem Cells Contain Functional Mitochondria?

التفاصيل البيبلوغرافية
العنوان: Do Extracellular Vesicles Derived from Mesenchymal Stem Cells Contain Functional Mitochondria?
المؤلفون: Zorova LD; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Kovalchuk SI; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia., Popkov VA; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Chernikov VP; A.P. Avtsyn Research Institute of Human Morphology, Moscow 117418, Russia., Zharikova AA; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119992, Russia., Khutornenko AA; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Zorov SD; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119992, Russia., Plokhikh KS; National Research Center, 'Kurchatov Institute', Moscow 123182, Russia., Zinovkin RA; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia., Evtushenko EA; Biological Faculty, Lomonosov Moscow State University, Moscow 119992, Russia., Babenko VA; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Pevzner IB; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Shevtsova YA; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia.; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119992, Russia., Goryunov KV; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Plotnikov EY; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Silachev DN; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Sukhikh GT; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia., Zorov DB; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.; V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Moscow 117997, Russia.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2022 Jul 03; Vol. 23 (13). Date of Electronic Publication: 2022 Jul 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Extracellular Vesicles*/metabolism , Mesenchymal Stem Cells*/metabolism, Flow Cytometry ; Mitochondria
مستخلص: Extracellular vesicles (EV) derived from stem cells have become an effective complement to the use in cell therapy of stem cells themselves, which has led to an explosion of research into the mechanisms of vesicle formation and their action. There is evidence demonstrating the presence of mitochondrial components in EV, but a definitive conclusion about whether EV contains fully functional mitochondria has not yet been made. In this study, two EV fractions derived from mesenchymal stromal stem cells (MSC) and separated by their size were examined. Flow cytometry revealed the presence of mitochondrial lipid components capable of interacting with mitochondrial dyes MitoTracker Green and 10-nonylacridine orange; however, the EV response to the probe for mitochondrial membrane potential was negative. Detailed analysis revealed components from all mitochondria compartments, including house-keeping mitochondria proteins and DNA as well as energy-related proteins such as membrane-localized proteins of complexes I, IV, and V, and soluble proteins from the Krebs cycle. When assessing the functional activity of mitochondria, high variability in oxygen consumption was noted, which was only partially attributed to mitochondrial respiratory activity. Our findings demonstrate that the EV contain all parts of mitochondria; however, their independent functionality inside EV has not been confirmed, which may be due either to the absence of necessary cofactors and/or the EV formation process and, probably the methodology of obtaining EV.
References: Nat Commun. 2021 Mar 30;12(1):1971. (PMID: 33785738)
J Cell Biochem. 2011 Feb;112(2):374-80. (PMID: 21268056)
J Control Release. 2021 Oct 10;338:505-526. (PMID: 34450196)
Front Bioeng Biotechnol. 2020 Feb 05;8:43. (PMID: 32117924)
Anal Biochem. 2018 Jul 1;552:50-59. (PMID: 28711444)
PLoS Biol. 2021 Apr 7;19(4):e3001166. (PMID: 33826607)
Cells. 2019 Mar 19;8(3):. (PMID: 30893822)
Molecules. 2018 Mar 19;23(3):. (PMID: 29562677)
Nat Commun. 2015 Oct 07;6:8472. (PMID: 26442449)
Mitochondrion. 2019 Nov;49:284-288. (PMID: 31100469)
Mitochondrion. 2019 Sep;48:16-30. (PMID: 30771504)
Nat Rev Neurosci. 2016 Mar;17(3):160-72. (PMID: 26891626)
Stem Cells Transl Med. 2022 Apr 29;11(4):372-382. (PMID: 35485440)
Int J Mol Sci. 2022 Jan 01;23(1):. (PMID: 35008907)
Nat Med. 2008 Sep;14(9):949-53. (PMID: 18690244)
Cell Transplant. 2019 Jul;28(7):801-812. (PMID: 31018669)
Eur J Biochem. 1992 Oct 1;209(1):267-73. (PMID: 1396703)
Nat Protoc. 2009;4(1):44-57. (PMID: 19131956)
Science. 2004 Feb 13;303(5660):1007-10. (PMID: 14963329)
Biol Rev Camb Philos Soc. 1966 Aug;41(3):445-502. (PMID: 5329743)
Mol Cell Proteomics. 2019 Feb;18(2):383-390. (PMID: 30373789)
Biochemistry (Mosc). 2015 May;80(5):542-8. (PMID: 26071771)
Q Rev Biophys. 1988 May;21(2):129-228. (PMID: 3043536)
Stem Cell Res Ther. 2020 Aug 8;11(1):345. (PMID: 32771052)
PLoS One. 2020 Jan 30;15(1):e0227949. (PMID: 31999742)
Physiology (Bethesda). 2019 May 1;34(3):169-177. (PMID: 30968753)
Physiol Rev. 2022 Apr 25;:. (PMID: 35466694)
Nat Rev Mol Cell Biol. 2006 Jan;7(1):9-19. (PMID: 16365634)
J Cell Biol. 1968 Jan;36(1):103-9. (PMID: 19806697)
Biomaterials. 2022 May;284:121486. (PMID: 35447404)
Hand Clin. 2017 May;33(2):237-242. (PMID: 28363291)
J Cell Physiol. 2022 May;237(5):2345-2356. (PMID: 35253232)
Stem Cell Res Ther. 2017 Oct 27;8(1):239. (PMID: 29078802)
Front Immunol. 2021 Mar 16;12:623973. (PMID: 33796099)
Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1283-8. (PMID: 16432190)
Stem Cells Transl Med. 2022 Apr 29;11(4):356-371. (PMID: 35485439)
Nat Protoc. 2016 Dec;11(12):2301-2319. (PMID: 27809316)
Nucleic Acids Res. 2009 Jan;37(1):1-13. (PMID: 19033363)
J Extracell Vesicles. 2015 May 14;4:27066. (PMID: 25979354)
Front Immunol. 2022 Apr 07;13:865888. (PMID: 35464407)
J Extracell Vesicles. 2018 Nov 23;7(1):1535750. (PMID: 30637094)
Exp Cell Res. 2010 Sep 10;316(15):2447-55. (PMID: 20599955)
FASEB J. 2020 Mar;34(3):3616-3630. (PMID: 31957088)
J Am Coll Cardiol. 2021 Mar 2;77(8):1073-1088. (PMID: 33632482)
Stem Cell Res Ther. 2018 Mar 9;9(1):63. (PMID: 29523213)
Anal Biochem. 2004 May 15;328(2):174-80. (PMID: 15113694)
Cell Metab. 2022 Apr 5;34(4):533-548.e12. (PMID: 35305295)
Nat Rev Cardiol. 2017 May;14(5):259-272. (PMID: 28150804)
Nat Med. 2012 Apr 15;18(5):759-65. (PMID: 22504485)
Nat Methods. 2021 Sep;18(9):1013-1026. (PMID: 34446922)
Exp Neurol. 2019 Jun;316:63-73. (PMID: 30981805)
Front Bioeng Biotechnol. 2020 Sep 10;8:997. (PMID: 33015001)
Nat Methods. 2016 Sep;13(9):731-40. (PMID: 27348712)
Front Cell Dev Biol. 2020 Nov 06;8:596079. (PMID: 33240892)
JACC CardioOncol. 2021 Jul 27;3(3):428-440. (PMID: 34604804)
Nature. 2010 Mar 4;464(7285):104-7. (PMID: 20203610)
معلومات مُعتمدة: 19-29-04090 Russian Foundation for Basic Research
فهرسة مساهمة: Keywords: ectosomes; exosomes; extracellular vesicles; mesenchymal stromal cells; mitochondria; stem cells
تواريخ الأحداث: Date Created: 20220709 Date Completed: 20220712 Latest Revision: 20220716
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9266972
DOI: 10.3390/ijms23137408
PMID: 35806411
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms23137408