دورية أكاديمية
Metagenomics Bioinformatic Pipeline.
| العنوان: | Metagenomics Bioinformatic Pipeline. |
|---|---|
| المؤلفون: | Garfias-Gallegos D; Laboratorio de Genómica Ecológica y Evolutiva, Langebio, Cinvestav, Mexico., Zirión-Martínez C; Laboratorio de Genómica Ecológica y Evolutiva, Langebio, Cinvestav, Mexico., Bustos-Díaz ED; Laboratorio de Evolución de la Diversidad Metabólica, Langebio, Cinvestav, Mexico., Arellano-Fernández TV; Laboratorio de Sistemas Genéticos, Langebio, Cinvestav, Mexico.; Escuela Nacional de Estudios Superiores, Unidad León, UNAM, León, Mexico., Lovaco-Flores JA; Escuela Nacional de Estudios Superiores, Unidad León, UNAM, León, Mexico.; BetterLab-C3, Irapuato, Mexico., Espinosa-Jaime A; Escuela Nacional de Estudios Superiores, Unidad León, UNAM, León, Mexico., Avelar-Rivas JA; Laboratorio de Sistemas Genéticos, Langebio, Cinvestav, Mexico., Sélem-Mójica N; BetterLab-C3, Irapuato, Mexico. nselem@matmor.unam.mx.; Centro de Ciencias Matemáticas, UNAM, Morelia, Mexico. nselem@matmor.unam.mx. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2512, pp. 153-179. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Metagenomics*/methods , Microbiota*, Computational Biology/methods ; Metagenome ; Sequence Analysis, DNA/methods ; Software |
| مستخلص: | Microbial communities' taxonomic and functional diversity has been broadly studied since sequencing technologies enabled faster and cheaper data obtainment. Nevertheless, the programming skills needed and the amount of software available may be overwhelming to someone trying to analyze these data. Here, we present a comprehensive and straightforward pipeline that takes shotgun metagenomics data through the needed steps to obtain valuable results. The raw data goes through a quality control process, metagenomic assembly, binning (the obtention of single genomes from a metagenome), taxonomic assignment, and taxonomic diversity analysis and visualization. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Assembly; Binning; Data visualization; Diversity; Metagenomics; Taxonomic assignment; Trimming |
| تواريخ الأحداث: | Date Created: 20220711 Date Completed: 20220713 Latest Revision: 20220817 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-0716-2429-6_10 |
| PMID: | 35818005 |
| قاعدة البيانات: | MEDLINE |
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