دورية أكاديمية
A high-resolution map of human RNA translation.
| العنوان: | A high-resolution map of human RNA translation. |
|---|---|
| المؤلفون: | Chothani SP; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Adami E; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore; Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany., Widjaja AA; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Langley SR; Lee Kong Chian School of Medicine, Nanyang Technological University, Clinical Sciences Building, Singapore 308232, Singapore., Viswanathan S; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Pua CJ; National Heart Research Institute Singapore (NHRIS), National Heart Centre Singapore, Singapore 169609, Singapore., Zhihao NT; Lee Kong Chian School of Medicine, Nanyang Technological University, Clinical Sciences Building, Singapore 308232, Singapore., Harmston N; Program in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore 169857, Singapore; Science Division, Yale-NUS College, Singapore 138527, Singapore., D'Agostino G; Lee Kong Chian School of Medicine, Nanyang Technological University, Clinical Sciences Building, Singapore 308232, Singapore., Whiffin N; Wellcome Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK., Mao W; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Ouyang JF; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Lim WW; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore; National Heart Research Institute Singapore (NHRIS), National Heart Centre Singapore, Singapore 169609, Singapore., Lim S; National Heart Research Institute Singapore (NHRIS), National Heart Centre Singapore, Singapore 169609, Singapore., Lee CQE; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Grubman A; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, VIC 3800, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, VIC 3800, Australia; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC 3800, Australia., Chen J; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, VIC 3800, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, VIC 3800, Australia; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC 3800, Australia., Kovalik JP; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Tryggvason K; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Polo JM; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, VIC 3800, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, VIC 3800, Australia; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, VIC 3800, Australia., Ho L; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore., Cook SA; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore; National Heart Research Institute Singapore (NHRIS), National Heart Centre Singapore, Singapore 169609, Singapore; London Institute of Medical Sciences, London W12 ONN, UK., Rackham OJL; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore; School of Biological Sciences, University of Southampton, Southampton, UK. Electronic address: owen.rackham@duke-nus.edu.sg., Schafer S; Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore, Singapore 169857, Singapore; National Heart Research Institute Singapore (NHRIS), National Heart Centre Singapore, Singapore 169609, Singapore. Electronic address: sebastian@duke-nus.edu.sg. |
| المصدر: | Molecular cell [Mol Cell] 2022 Aug 04; Vol. 82 (15), pp. 2885-2899.e8. Date of Electronic Publication: 2022 Jul 15. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 9802571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4164 (Electronic) Linking ISSN: 10972765 NLM ISO Abbreviation: Mol Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge Ma : Cell Press Original Publication: Cambridge, Mass. : Cell Press, c1997- |
| مواضيع طبية MeSH: | Gene Expression Regulation* , Ribosomes*/genetics , Ribosomes*/metabolism, Genome, Human/genetics ; Humans ; Open Reading Frames/genetics ; Protein Biosynthesis ; Proteins/metabolism ; RNA/metabolism |
| مستخلص: | Translated small open reading frames (smORFs) can have important regulatory roles and encode microproteins, yet their genome-wide identification has been challenging. We determined the ribosome locations across six primary human cell types and five tissues and detected 7,767 smORFs with translational profiles matching those of known proteins. The human genome was found to contain highly cell-type- and tissue-specific smORFs and a subset that encodes highly conserved amino acid sequences. Changes in the translational efficiency of upstream-encoded smORFs (uORFs) and the corresponding main ORFs predominantly occur in the same direction. Integration with 456 mass-spectrometry datasets confirms the presence of 603 small peptides at the protein level in humans and provides insights into the subcellular localization of these small proteins. This study provides a comprehensive atlas of high-confidence translated smORFs derived from primary human cells and tissues in order to provide a more complete understanding of the translated human genome. Competing Interests: Declaration of interests S.S. and S.A.C. are co-founders and shareholders of Enleofen Bio Pte Ltd. O.J.L.R. and J.M.P. are SAB members and shareholders of Mogrify Ltd. All other authors declare no competing interests. (Copyright © 2022 Elsevier Inc. All rights reserved.) |
| معلومات مُعتمدة: | MC_U120085815 United Kingdom MRC_ Medical Research Council; IRSP 55008732 United States HHMI Howard Hughes Medical Institute |
| فهرسة مساهمة: | Keywords: Ribo-seq; Ribosome profiling; dORFs; lncRNAs; smORFs; translation; uORFs; untranslated regions |
| المشرفين على المادة: | 0 (Proteins) 63231-63-0 (RNA) |
| تواريخ الأحداث: | Date Created: 20220716 Date Completed: 20220809 Latest Revision: 20230315 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.molcel.2022.06.023 |
| PMID: | 35841888 |
| قاعدة البيانات: | MEDLINE |
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