دورية أكاديمية
أعرض في EDS

Properdin produced by dendritic cells contributes to the activation of T cells.

التفاصيل البيبلوغرافية
العنوان: Properdin produced by dendritic cells contributes to the activation of T cells.
المؤلفون: van Essen MF; Div of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Leiden, The Netherlands., Schlagwein N; Div of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Leiden, The Netherlands., van Gijlswijk-Janssen DJ; Div of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Leiden, The Netherlands., Ruben JM; Div of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Leiden, The Netherlands., van Kooten C; Div of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: kooten@lumc.nl.
مؤلفون مشاركون: COMBAT consortium; Div of Nephrology and Transplant Medicine, Dept. of Medicine, Leiden University Medical Center, Leiden, The Netherlands.
المصدر: Immunobiology [Immunobiology] 2022 Jul; Vol. 227 (4), pp. 152246. Date of Electronic Publication: 2022 Jul 09.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8002742 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3279 (Electronic) Linking ISSN: 01712985 NLM ISO Abbreviation: Immunobiology Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005->: Amsterdam : Elsevier
Original Publication: Stuttgart ; New York, Fischer.
مواضيع طبية MeSH: Kidney Transplantation* , Properdin*/genetics , Properdin*/metabolism, Cells, Cultured ; Dendritic Cells ; Humans ; RNA, Small Interfering ; T-Lymphocytes
مستخلص: The complement system does not only play an important role in the defence against microorganism and pathogens, but also contributes to the regulation of innate and adaptive immunity. Especially activation fragments C3a and C5a and complement activation at the interface of antigen presenting cell (APC) and T cell, were shown to have a role in T cell activation and proliferation. Whereas most complement factors are produced by the liver, properdin, a positive regulator of the C3 convertase, is mainly produced by myeloid cells. Here we show that properdin can be detected in myeloid cell infiltrate during human renal allograft rejection. In vitro, properdin is produced and secreted by human immature dendritic cells (iDCs), which is further increased by CD40-L-matured DCs (mDCs). Transfection with a specific properdin siRNA reduced properdin secretion by iDCs and mDCs, without affecting the expression of co-stimulatory markers CD80 and CD86. Co-culture of properdin siRNA-transfected iDCs and mDCs with human allogeneic T cells resulted in reduced T cell proliferation, especially under lower DC-T cell ratio's (1:30 and 1:90 ratio). In addition, T cell cytokines were altered, including a reduced TNF-α and IL-17 secretion by T cells co-cultured with properdin siRNA-transfected iDCs. Taken together, these results indicate a local role for properdin during the interaction of DCs and allogeneic T cells, contributing to the shaping of T cell proliferation and activation.
(Copyright © 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.)
فهرسة مساهمة: Keywords: Complement system; Dendritic cells; Inflammation; Properdin; T cells
المشرفين على المادة: 0 (RNA, Small Interfering)
11016-39-0 (Properdin)
تواريخ الأحداث: Date Created: 20220717 Date Completed: 20220802 Latest Revision: 20220921
رمز التحديث: 20231215
DOI: 10.1016/j.imbio.2022.152246
PMID: 35843030
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-3279
DOI:10.1016/j.imbio.2022.152246