دورية أكاديمية
Fibulin-3 sponges Tiam1 to manipulate MMP-7 activity through β-catenin signaling in oral squamous cell carcinoma.
| العنوان: | Fibulin-3 sponges Tiam1 to manipulate MMP-7 activity through β-catenin signaling in oral squamous cell carcinoma. |
|---|---|
| المؤلفون: | Guo H; School and Hospital of Stomatology, Tianjin Medical University, Tianjin, China., Ji Y; Hexi Women & Children Healthcare and Family Planning Service Center, Tianjin, China., Zhang B; Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China. bozhang@tmu.edu.cn., Huang X; School and Hospital of Stomatology, Tianjin Medical University, Tianjin, China. xinhuang@tmu.edu.cn. |
| المصدر: | Medical oncology (Northwood, London, England) [Med Oncol] 2022 Jul 19; Vol. 39 (10), pp. 154. Date of Electronic Publication: 2022 Jul 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 9435512 Publication Model: Electronic Cited Medium: Internet ISSN: 1559-131X (Electronic) Linking ISSN: 13570560 NLM ISO Abbreviation: Med Oncol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : New York : Springer Original Publication: Northwood, Middlesex, England : Science and Technology Letters, c1994- |
| مواضيع طبية MeSH: | Extracellular Matrix Proteins*/metabolism , Head and Neck Neoplasms*/metabolism , Mouth Neoplasms*/metabolism , Squamous Cell Carcinoma of Head and Neck*/metabolism , T-Lymphoma Invasion and Metastasis-inducing Protein 1*/metabolism, Calcium-Binding Proteins/metabolism ; Cell Line, Tumor ; Cell Movement/physiology ; Epithelial-Mesenchymal Transition ; Humans ; Matrix Metalloproteinase 7/metabolism ; RNA, Messenger/metabolism ; Signal Transduction ; beta Catenin/metabolism |
| مستخلص: | Oral squamous cell carcinoma (named OSCC) is considered the most frequent malignancy in oral cavity, which has become a rapid increasing problem for the global public health with unclear molecular mechanism. Previously, Tiam1 (T-lymphoma invasion and metastasis inducing factor 1) has been reported as a potential oncogene for OSCC. Here, we in-depth explored its signaling mechanism for the disorder. The mRNA and protein expression levels of primary differentially expressed genes (Tiam1, Fibulin-3, and MMP-7) were measured in different TNM stages of OSCC patients using RT-PCR and ELISA methods. Based on the analysis of human OSCC cell line CAL27, the relationships between these factors have been further investigated and the interactions were also examined. The luciferase reporter assay was established for the promoter region of MMP-7. Both the epithelial (E-cadherin) and mesenchymal protein markers (Vimentin and Snail) expressions were examined using western blotting. The mRNA and protein activities of Fibulin-3 declined as the increase of TNM stage. Inversely, the mRNA and protein levels of Tiam1 and MMP-7 elevated significantly as OSCC progressed. Tiam1 transfection in CAL27 cells stimulated the expression of MMP-7 by accelerating the nuclear translocation of β-catenin, which was opposite to the functions of Fibulin-3. Moreover, Tiam1 interacted directly with Fibulin-3. The Tiam1 induced OSCC epithelial-mesenchymal transition (EMT) via MMP-7 activation, which was dependent on the direct binding of β-catenin at the promoter region. Collectively, these results indicated that Tiam1 competed with Fibulin-3 for nuclear β-catenin translocation, which subsequently stimulated MMP-7 expression by TCF-4 domain interaction following EMT initiation in OSCC development. Our systematical work hypothesized an innovative signaling cassette for OSCC progression, which provided beneficial references for future clinical study. (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| معلومات مُعتمدة: | 82070687 National Natural Science Foundation of China |
| فهرسة مساهمة: | Keywords: Fibulin-3; MMP-7; Oral squamous cell carcinoma; Tiam1; β-catenin |
| المشرفين على المادة: | 0 (CTNNB1 protein, human) 0 (Calcium-Binding Proteins) 0 (EFEMP1 protein, human) 0 (Extracellular Matrix Proteins) 0 (RNA, Messenger) 0 (T-Lymphoma Invasion and Metastasis-inducing Protein 1) 0 (TIAM1 protein, human) 0 (beta Catenin) EC 3.4.24.23 (MMP7 protein, human) EC 3.4.24.23 (Matrix Metalloproteinase 7) |
| تواريخ الأحداث: | Date Created: 20220719 Date Completed: 20220721 Latest Revision: 20220727 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s12032-022-01746-4 |
| PMID: | 35852664 |
| قاعدة البيانات: | MEDLINE |
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