دورية أكاديمية
Acinetobacter baumannii Genomic Sequence-Based Core Genome Multilocus Sequence Typing Using Ridom SeqSphere+ and Antimicrobial Susceptibility Prediction in ARESdb.
| العنوان: | Acinetobacter baumannii Genomic Sequence-Based Core Genome Multilocus Sequence Typing Using Ridom SeqSphere+ and Antimicrobial Susceptibility Prediction in ARESdb. |
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| المؤلفون: | Fida M; Division of Infectious Diseases, Department of Medicine, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA., Cunningham SA; Division of Clinical Microbiology, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA., Beisken S; Ares Genetics GmbH, Vienna, Austria., Posch AE; Ares Genetics GmbH, Vienna, Austria., Chia N; Center for Individualized Medicine, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA.; Department of Surgery, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA., Jeraldo PR; Center for Individualized Medicine, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA.; Department of Surgery, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA., Murphy MP; Division of Clinical Microbiology, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA., Zinsmaster NM; Division of Clinical Microbiology, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA., Patel R; Division of Infectious Diseases, Department of Medicine, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA.; Division of Clinical Microbiology, Mayo Clinicgrid.66875.3agrid.470142.4grid.66875.3a, Rochester, Minnesota, USA. |
| مؤلفون مشاركون: | Antibacterial Resistance Leadership Group |
| المصدر: | Journal of clinical microbiology [J Clin Microbiol] 2022 Aug 17; Vol. 60 (8), pp. e0053322. Date of Electronic Publication: 2022 Jul 12. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Acinetobacter baumannii*/genetics , Anti-Infective Agents*, Genome, Bacterial/genetics ; Genomics ; Humans ; Multilocus Sequence Typing/methods |
| مستخلص: | Whole-genome sequencing (WGS) is rapidly replacing traditional typing methods for the investigation of infectious disease outbreaks. Additionally, WGS data are being used to predict phenotypic antimicrobial susceptibility. Acinetobacter baumannii, which is often multidrug-resistant, is a significant culprit in outbreaks in health care settings. A well-characterized collection of A. baumannii was studied using core genome multilocus sequence typing (cgMLST). Seventy-two isolates previously typed by PCR-electrospray ionization mass spectrometry (PCR/ESI-MS) provided by the Antimicrobial Resistance Leadership Group (ARLG) were analyzed using a clinical microbiology laboratory developed workflow for cgMLST with genomic susceptibility prediction performed using the ARESdb platform. Previously performed PCR/ESI-MS correlated with cgMLST using relatedness thresholds of allelic differences of ≤9 and ≤200 allelic differences in 78 and 94% of isolates, respectively. Categorical agreement between genotypic and phenotypic antimicrobial susceptibility across a panel of 11 commonly used drugs was 89%, with minor, major, and very major error rates of 8%, 11%, and 1%, respectively. |
| References: | Antimicrob Agents Chemother. 2006 Dec;50(12):4114-23. (PMID: 17000742) J Clin Microbiol. 2016 Sep;54(9):2391-4. (PMID: 27358465) Clin Microbiol Rev. 2008 Jul;21(3):538-82. (PMID: 18625687) Emerg Infect Dis. 2019 Jan;25(1):186-187. (PMID: 30561303) J Crit Care. 2011 Oct;26(5):453-459. (PMID: 21439763) Int J Antimicrob Agents. 2021 Oct;58(4):106404. (PMID: 34298091) J Clin Microbiol. 2014 Jul;52(7):2479-86. (PMID: 24789177) J Clin Microbiol. 2020 Jun 24;58(7):. (PMID: 32295890) J Clin Microbiol. 2021 Nov 18;59(12):e0021321. (PMID: 34550809) New Microbiol. 2014 Apr;37(2):185-91. (PMID: 24858645) PLoS One. 2017 Jun 2;12(6):e0179003. (PMID: 28575112) J Glob Antimicrob Resist. 2019 Jun;17:245-249. (PMID: 30553929) Chest. 1999 May;115(5):1378-82. (PMID: 10334156) BMC Bioinformatics. 2010 Dec 10;11:595. (PMID: 21143983) Nat Biotechnol. 2013 Apr;31(4):294-6. (PMID: 23563421) Infect Genet Evol. 2013 Jun;16:38-53. (PMID: 23357583) Euro Surveill. 2015 Jul 16;20(28):. (PMID: 26212142) Microb Pathog. 2020 Feb;139:103887. (PMID: 31765766) J Clin Microbiol. 1998 Jul;36(7):1938-41. (PMID: 9650940) J Clin Microbiol. 2014 Jul;52(7):2365-70. (PMID: 24759713) Front Microbiol. 2019 May 03;10:930. (PMID: 31130931) PLoS One. 2017 Jun 8;12(6):e0179228. (PMID: 28594944) PLoS One. 2015 Apr 09;10(4):e0123298. (PMID: 25856198) Int J Mol Sci. 2021 Dec 02;22(23):. (PMID: 34884852) Lancet Microbe. 2022 Apr;3(4):e244-e245. (PMID: 35544058) mSystems. 2021 Aug 31;6(4):e0062621. (PMID: 34282943) |
| معلومات مُعتمدة: | UM1 AI104681 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: antimicrobial resistance; cgMLST; genotypic resistance; whole-genome sequencing |
| المشرفين على المادة: | 0 (Anti-Infective Agents) |
| تواريخ الأحداث: | Date Created: 20220721 Date Completed: 20220819 Latest Revision: 20240906 |
| رمز التحديث: | 20240906 |
| مُعرف محوري في PubMed: | PMC9383114 |
| DOI: | 10.1128/jcm.00533-22 |
| PMID: | 35862760 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-660X |
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| DOI: | 10.1128/jcm.00533-22 |