دورية أكاديمية
أعرض في EDS

Golgi scaffold protein PAQR3 as a candidate suppressor of gastric cardia adenocarcinoma via regulating TGF-β/Smad pathway.

التفاصيل البيبلوغرافية
العنوان: Golgi scaffold protein PAQR3 as a candidate suppressor of gastric cardia adenocarcinoma via regulating TGF-β/Smad pathway.
المؤلفون: Wu YL; Zhejiang Cancer Institute, Cancer Hospital of the University of Chinese Academy of Sciences; Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Hangzhou, China.; Department of anaesthesiology, Cancer Hospital of the University of Chinese Academy of Sciences; Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Hangzhou, China., Hong LL; Zhejiang Cancer Institute, Cancer Hospital of the University of Chinese Academy of Sciences; Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Hangzhou, China., Ling ZN; Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China., Hu XY; Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou, China., Liu Z; Zhejiang Cancer Institute, Cancer Hospital of the University of Chinese Academy of Sciences; Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Hangzhou, China., Li P; Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou, China., Ling ZQ; Zhejiang Cancer Institute, Cancer Hospital of the University of Chinese Academy of Sciences; Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Hangzhou, China.
المصدر: Journal of clinical laboratory analysis [J Clin Lab Anal] 2022 Sep; Vol. 36 (9), pp. e24617. Date of Electronic Publication: 2022 Jul 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: United States NLM ID: 8801384 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-2825 (Electronic) Linking ISSN: 08878013 NLM ISO Abbreviation: J Clin Lab Anal Subsets: MEDLINE
أسماء مطبوعة: Publication: Hoboken, N.J. : Wiley
Original Publication: New York : Alan R. Liss, Inc., c1987-
مواضيع طبية MeSH: Adenocarcinoma*/pathology , Helicobacter Infections* , Helicobacter pylori* , Stomach Neoplasms*/pathology, Intracellular Signaling Peptides and Proteins/*metabolism , Membrane Proteins/*metabolism, Cardia/metabolism ; Cardia/pathology ; Cell Line, Tumor ; Humans ; Smad Proteins/metabolism ; Transforming Growth Factor beta/metabolism
مستخلص: Objectives: To investigate the function of PAQR3 in gastric cardia adenocarcinoma (GCA) and understand the possible mechanism of PAQR3 in regulating epithelial-mesenchymal transition (EMT).
Methods: We detected PAQR3 protein in 146 GCA tissues and paired normal adjacent tissues (PNTs) specimens using immunohistochemical analysis, and explored its clinical significance. The expression levels of PAQR3 protein in 20 GCA tissues, their paired PNTs, HGC27, SGC7901, and GES-1 cells were analyzed by Western blot. Wild-type PAQR3 was overexpressed in HGC27 cells. The effects of PAQR3 overexpression on the function of HGC27 cells and its underlying mechanisms were then analyzed through a series of cell and molecular biology experiments.
Results: PAQR3 was significantly down-regulated in GCA tissues when compared with paired PNTs (p < 0.0001). The expression level of PAQR3 in GCA tissues was significantly negatively correlated with Helicobacter pylori infection (p = 0.000), venous invasion (p = 0.000), invasion depth (p = 0.000), lymph node metastasis (p = 0.022), tumor stage (p = 0.000), and patient survival (p = 0.009). Downregulation of PAQR3 was highly correlated with increased EMT signature and activated TGF-β/Smad pathway in GCA tissues. Overexpression of PAQR3 in HGC27 cells negatively regulates its cellular functions, such as cell proliferation and migration, and suppresses EMT. Mechanistically, overexpression of PAQR3 significantly down-regulates the protein expression levels of TGF-1, p-Smad2, and p-Smad3 in HGC27 cells.
Conclusion: PAQR3 was significantly down-regulated in GCA tissues, HGC27, and SGC7901 cells. PAQR3 significantly inhibits the proliferation, migration, and invasion of HGC27 cells. Mechanistically, PAQR3 can inhibit the EMT process in HGC27 cells by regulating TGF-β/Smad signaling pathway.
(© 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.)
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معلومات مُعتمدة: LGD20H160003 Zhejiang Provincial Public Welfare Technology Research Plan Project; 2019ZD025 Zhejiang Medical and Health Science and Technology Project; 20IRTSTHN026 University of Henan Province; Zjhrss2014-150 Zhejiang Provincial Program for Training and Development Project; Zjwjw2021-40 Zhejiang Province Health Leader Talent; [2019]-3 Zhejiang Provincial Ten Thousand Talents Plan; LQ19H160005 Zhejiang Provincial Natural Science Foundation; LZ18H160002 Zhejiang Provincial Natural Science Foundation; WKJ-ZJ-2117 National Health Commission Science Research Fund-Zhejiang Provincial Health Key Science and Technology Plan Project; 81972908 National Natural Science Foundation of China
فهرسة مساهمة: Keywords: PAQR3; TGF-β/Smad signaling pathway; epithelial-mesenchymal transition; gastric cardia adenocarcinoma; prognosis
المشرفين على المادة: 0 (Intracellular Signaling Peptides and Proteins)
0 (Membrane Proteins)
0 (PAQR3 protein, human)
0 (Smad Proteins)
0 (Transforming Growth Factor beta)
تواريخ الأحداث: Date Created: 20220723 Date Completed: 20220912 Latest Revision: 20220921
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC9459307
DOI: 10.1002/jcla.24617
PMID: 35870178
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-2825
DOI:10.1002/jcla.24617