دورية أكاديمية
Age-Related Alterations in Macrophage Distribution and Function Are Associated With Delayed Cutaneous Wound Healing.
| العنوان: | Age-Related Alterations in Macrophage Distribution and Function Are Associated With Delayed Cutaneous Wound Healing. |
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| المؤلفون: | Dube CT; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.; Epithelial Epigenetics and Development Laboratory, ASTAR Skin Research Labs, Singapore, Singapore., Ong YHB; Epithelial Epigenetics and Development Laboratory, ASTAR Skin Research Labs, Singapore, Singapore., Wemyss K; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.; Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom., Krishnan S; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.; Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom., Tan TJ; Epithelial Epigenetics and Development Laboratory, ASTAR Skin Research Labs, Singapore, Singapore., Janela B; Skin Immunology Laboratory, ASTAR Skin Research Labs, Singapore, Singapore.; Skin Immuno-Monitoring Platform , Skin Research Institute of Singapore, Singapore, Singapore., Grainger JR; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.; Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom., Ronshaugen M; School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom., Mace KA; School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom., Lim CY; Epithelial Epigenetics and Development Laboratory, ASTAR Skin Research Labs, Singapore, Singapore.; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. |
| المصدر: | Frontiers in immunology [Front Immunol] 2022 Jul 08; Vol. 13, pp. 943159. Date of Electronic Publication: 2022 Jul 08 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Skin*/metabolism , Wound Healing*/genetics, Aged ; Animals ; Humans ; Inflammation/metabolism ; Macrophages/metabolism ; Mice ; Mice, Inbred C57BL |
| مستخلص: | Ageing-related delays and dysregulated inflammation in wound healing are well-documented in both human and animal models. However, cellular and molecular changes underlying this impairment in healing progression are not fully understood. In this study, we characterised ageing-associated changes to macrophages in wounds of young and aged mice and investigated transcriptomic differences that may impact the progression of wound healing. Full-thickness wounds created on the dorsum of C57BL/6J young and aged mice were excised on Days 3 and 7 post-wounding for analysis by immunohistochemistry, flow cytometry, and RNA sequencing. Our data revealed that macrophages were significantly reduced in aged wounds in comparison to young. Functional transcriptomic analyses showed that macrophages from aged wounds exhibited significantly reduced expression of cell cycle, DNA replication, and repair pathway genes. Furthermore, we uncovered an elevated pro-inflammatory gene expression program in the aged macrophages correlated with poor inflammation resolution and excessive tissue damage observed in aged wounds. Altogether, our work provides insights into how poorly healing aged wounds are phenotypically defined by the presence of macrophages with reduced proliferative capacity and an exacerbated inflammatory response, both of which are pathways that can be targeted to improve healing in the elderly. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Dube, Ong, Wemyss, Krishnan, Tan, Janela, Grainger, Ronshaugen, Mace and Lim.) |
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| معلومات مُعتمدة: | United Kingdom BHF_ British Heart Foundation |
| فهرسة مساهمة: | Keywords: ageing skin; inflammation; macrophages; myeloid cells; proliferation; wound healing |
| تواريخ الأحداث: | Date Created: 20220725 Date Completed: 20220726 Latest Revision: 20240901 |
| رمز التحديث: | 20240901 |
| مُعرف محوري في PubMed: | PMC9304927 |
| DOI: | 10.3389/fimmu.2022.943159 |
| PMID: | 35874681 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2022.943159 |