دورية أكاديمية
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The giant mimivirus 1.2 Mb genome is elegantly organized into a 30-nm diameter helical protein shield.

التفاصيل البيبلوغرافية
العنوان: The giant mimivirus 1.2 Mb genome is elegantly organized into a 30-nm diameter helical protein shield.
المؤلفون: Villalta A; Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique & Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479, IM2B), Marseille, France., Schmitt A; Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique & Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479, IM2B), Marseille, France., Estrozi LF; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS), Grenoble, France., Quemin ERJ; Centre for Structural Systems Biology, Leibniz Institute for Experimental Virology (HPI), University of Hamburg, Hamburg, Germany., Alempic JM; Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique & Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479, IM2B), Marseille, France., Lartigue A; Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique & Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479, IM2B), Marseille, France., Pražák V; Centre for Structural Systems Biology, Leibniz Institute for Experimental Virology (HPI), University of Hamburg, Hamburg, Germany.; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom., Belmudes L; Univ. Grenoble Alpes, CEA, INSERM, IRIG, BGE, Grenoble, France., Vasishtan D; Centre for Structural Systems Biology, Leibniz Institute for Experimental Virology (HPI), University of Hamburg, Hamburg, Germany.; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom., Colmant AMG; Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique & Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479, IM2B), Marseille, France., Honoré FA; Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique & Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479, IM2B), Marseille, France., Couté Y; Univ. Grenoble Alpes, CEA, INSERM, IRIG, BGE, Grenoble, France., Grünewald K; Centre for Structural Systems Biology, Leibniz Institute for Experimental Virology (HPI), University of Hamburg, Hamburg, Germany.; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom., Abergel C; Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique & Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479, IM2B), Marseille, France.
المصدر: ELife [Elife] 2022 Jul 28; Vol. 11. Date of Electronic Publication: 2022 Jul 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
مواضيع طبية MeSH: Giant Viruses*/genetics , Mimiviridae*/genetics, Capsid/metabolism ; Cryoelectron Microscopy/methods ; Genome, Viral ; Nucleoproteins/genetics ; Nucleoproteins/metabolism ; Oxidoreductases/metabolism
مستخلص: Mimivirus is the prototype of the Mimiviridae family of giant dsDNA viruses. Little is known about the organization of the 1.2 Mb genome inside the membrane-limited nucleoid filling the ~0.5 µm icosahedral capsids. Cryo-electron microscopy, cryo-electron tomography, and proteomics revealed that it is encased into a ~30-nm diameter helical protein shell surprisingly composed of two GMC-type oxidoreductases, which also form the glycosylated fibrils decorating the capsid. The genome is arranged in 5- or 6-start left-handed super-helices, with each DNA-strand lining the central channel. This luminal channel of the nucleoprotein fiber is wide enough to accommodate oxidative stress proteins and RNA polymerase subunits identified by proteomics. Such elegant supramolecular organization would represent a remarkable evolutionary strategy for packaging and protecting the genome, in a state ready for immediate transcription upon unwinding in the host cytoplasm. The parsimonious use of the same protein in two unrelated substructures of the virion is unexpected for a giant virus with thousand genes at its disposal.
Competing Interests: AV, AS, LE, EQ, JA, AL, VP, LB, DV, AC, FH, YC, KG, CA No competing interests declared
(© 2022, Villalta, Schmitt, Estrozi et al.)
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; 107806/Z/15/Z United Kingdom WT_ Wellcome Trust
فهرسة مساهمة: Keywords: cryo-EM; cryo-ET; giant virus; infectious disease; microbiology; mimivirus; molecular biophysics; proteomics; structural biology; viral dsDNA organization; viruses
المشرفين على المادة: 0 (Nucleoproteins)
EC 1.- (Oxidoreductases)
تواريخ الأحداث: Date Created: 20220728 Date Completed: 20220926 Latest Revision: 20240517
رمز التحديث: 20240517
مُعرف محوري في PubMed: PMC9512402
DOI: 10.7554/eLife.77607
PMID: 35900198
قاعدة البيانات: MEDLINE
الوصف
تدمد:2050-084X
DOI:10.7554/eLife.77607