دورية أكاديمية
Cellular recovery after prolonged warm ischaemia of the whole body.
| العنوان: | Cellular recovery after prolonged warm ischaemia of the whole body. |
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| المؤلفون: | Andrijevic D; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Vrselja Z; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Lysyy T; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA.; Department of Surgery, Yale School of Medicine New Haven, New Haven, CT, USA., Zhang S; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA.; Department of Genetics, Yale School of Medicine, New Haven, CT, USA., Skarica M; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Spajic A; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Dellal D; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA.; Department of Biomedical Engineering, Yale University, New Haven, CT, USA., Thorn SL; Yale Translational Research Imaging Center, Department of Medicine, Yale School of Medicine, New Haven, CT, USA., Duckrow RB; Department of Neurology, Yale University School of Medicine, New Haven, CT, USA., Ma S; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Duy PQ; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA.; Department of Neurosurgery, Yale School of Medicine, New Haven, CT, USA.; Medical Scientist Training Program (MD-PhD), Yale School of Medicine, New Haven, CT, USA., Isiktas AU; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Liang D; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Li M; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Kim SK; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA., Daniele SG; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA.; Medical Scientist Training Program (MD-PhD), Yale School of Medicine, New Haven, CT, USA., Banu K; Department of Nephrology, Yale School of Medicine, New Haven, CT, USA., Perincheri S; Department of Pathology, Yale School of Medicine, New Haven, CT, USA., Menon MC; Department of Nephrology, Yale School of Medicine, New Haven, CT, USA., Huttner A; Department of Pathology, Yale School of Medicine, New Haven, CT, USA., Sheth KN; Department of Neurology, Yale University School of Medicine, New Haven, CT, USA.; Department of Neurosurgery, Yale School of Medicine, New Haven, CT, USA., Gobeske KT; Department of Neurology, Yale University School of Medicine, New Haven, CT, USA., Tietjen GT; Department of Surgery, Yale School of Medicine New Haven, New Haven, CT, USA.; Department of Biomedical Engineering, Yale University, New Haven, CT, USA., Zaveri HP; Department of Neurology, Yale University School of Medicine, New Haven, CT, USA., Latham SR; Interdisciplinary Center for Bioethics, Yale University, New Haven, CT, USA., Sinusas AJ; Department of Genetics, Yale School of Medicine, New Haven, CT, USA.; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.; Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA.; Department of Radiology and Biomedical Imaging, Yale School of Medicine, New Haven, CT, USA., Sestan N; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA. nenad.sestan@yale.edu.; Department of Genetics, Yale School of Medicine, New Haven, CT, USA. nenad.sestan@yale.edu.; Department of Psychiatry, Yale School of Medicine, New Haven, CT, USA. nenad.sestan@yale.edu.; Department of Comparative Medicine, Yale School of Medicine, New Haven, CT, USA. nenad.sestan@yale.edu.; Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale School of Medicine, New Haven, CT, USA. nenad.sestan@yale.edu.; Yale Child Study Center, New Haven, CT, USA. nenad.sestan@yale.edu. |
| المصدر: | Nature [Nature] 2022 Aug; Vol. 608 (7922), pp. 405-412. Date of Electronic Publication: 2022 Aug 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Basingstoke : Nature Publishing Group Original Publication: London, Macmillan Journals ltd. |
| مواضيع طبية MeSH: | Cell Survival* , Cytoprotection* , Perfusion*/methods , Swine*/anatomy & histology , Warm Ischemia*, Animals ; Cell Death ; Gene Expression Profiling ; Ischemia/metabolism ; Ischemia/pathology ; Ischemia/prevention & control ; Organ Specificity |
| مستخلص: | After cessation of blood flow or similar ischaemic exposures, deleterious molecular cascades commence in mammalian cells, eventually leading to their death 1,2 . Yet with targeted interventions, these processes can be mitigated or reversed, even minutes or hours post mortem, as also reported in the isolated porcine brain using BrainEx technology 3 . To date, translating single-organ interventions to intact, whole-body applications remains hampered by circulatory and multisystem physiological challenges. Here we describe OrganEx, an adaptation of the BrainEx extracorporeal pulsatile-perfusion system and cytoprotective perfusate for porcine whole-body settings. After 1 h of warm ischaemia, OrganEx application preserved tissue integrity, decreased cell death and restored selected molecular and cellular processes across multiple vital organs. Commensurately, single-nucleus transcriptomic analysis revealed organ- and cell-type-specific gene expression patterns that are reflective of specific molecular and cellular repair processes. Our analysis comprises a comprehensive resource of cell-type-specific changes during defined ischaemic intervals and perfusion interventions spanning multiple organs, and it reveals an underappreciated potential for cellular recovery after prolonged whole-body warm ischaemia in a large mammal. (© 2022. The Author(s), under exclusive licence to Springer Nature Limited.) |
| التعليقات: | Comment in: Nature. 2022 Aug;608(7922):273-274. (PMID: 35922486) Comment in: Nat Rev Nephrol. 2022 Oct;18(10):605. (PMID: 36042350) Comment in: Gastroenterology. 2023 Apr;164(4):696-697. (PMID: 36400180) |
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| معلومات مُعتمدة: | F30 HD106694 United States HD NICHD NIH HHS; R01 DK122164 United States DK NIDDK NIH HHS; RF1 MH117064 United States MH NIMH NIH HHS; R21 DK128662 United States DK NIDDK NIH HHS; R01 MH113257 United States MH NIMH NIH HHS; UL1 TR001863 United States TR NCATS NIH HHS; T32 GM136651 United States GM NIGMS NIH HHS |
| تواريخ الأحداث: | Date Created: 20220803 Date Completed: 20220812 Latest Revision: 20230327 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC9518831 |
| DOI: | 10.1038/s41586-022-05016-1 |
| PMID: | 35922506 |
| قاعدة البيانات: | MEDLINE |
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| DOI: | 10.1038/s41586-022-05016-1 |