دورية أكاديمية
Markers of ovarian reserve are associated with reproductive age acceleration in granulosa cells from IVF patients.
| العنوان: | Markers of ovarian reserve are associated with reproductive age acceleration in granulosa cells from IVF patients. |
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| المؤلفون: | Knight AK; Division of Research, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA., Hipp HS; Division of Reproductive Endocrinology and Infertility, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA., Abhari S; Division of Reproductive Endocrinology and Infertility, Johns Hopkins Medicine, Timonium, MD, USA., Gerkowicz SA; IVF MD, Boca Raton, FL, USA., Katler QS; Division of Reproductive Endocrinology and Infertility, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA., McKenzie LJ; Division of Reproductive Endocrinology and Infertility, Baylor College of Medicine, Houston, TX, USA., Shang W; Division of Reproductive Endocrinology and Infertility, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA., Smith AK; Division of Research, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA., Spencer JB; Division of Reproductive Endocrinology and Infertility, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA. |
| المصدر: | Human reproduction (Oxford, England) [Hum Reprod] 2022 Sep 30; Vol. 37 (10), pp. 2438-2445. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8701199 Publication Model: Print Cited Medium: Internet ISSN: 1460-2350 (Electronic) Linking ISSN: 02681161 NLM ISO Abbreviation: Hum Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford, UK : Oxford University Press Original Publication: Oxford ; Washington, DC : Published for the European Society of Human Reproduction and Embryology by IRL Press, [c1986- |
| مواضيع طبية MeSH: | Ovarian Reserve*, Acceleration ; Anti-Mullerian Hormone ; Cross-Sectional Studies ; Female ; Fertilization in Vitro ; Granulosa Cells ; Humans |
| مستخلص: | Study Question: Is reproductive aging in granulosa cells associated with markers of ovarian reserve? Summary Answer: Age acceleration was associated with anti-Mullerian hormone (AMH) levels, antral follicle count (AFC), oocyte yield and maturity, and the number of successfully fertilized embryos. What Is Known Already: The rate of reproductive aging varies among women of the same age. DNA methylation can be used to predict epigenetic age in a variety of tissues. Study Design, Size, Duration: This was a cross-sectional study of 70 women at the time of oocyte retrieval. Participants/materials, Setting, Methods: The 70 participants were recruited for this study at an academic medical center and they provided follicular fluid samples at the time of oocyte retrieval. Granulosa cells were isolated and assessed on the MethylationEPIC array. Linear regression was used to evaluate the associations between DNA methylation-based age predictions from granulosa cells and chronological age. Age acceleration was calculated as the residual of regressing DNA methylation-based age on chronological age. Linear regressions were used to determine the associations between age acceleration and markers of ovarian reserve and IVF cycle outcomes. Main Results and the Role of Chance: Participants were a mean of 36.7 ± 3.9 years old. In regards to race, 54% were white, 19% were African American and 27% were of another background. Age acceleration was normally distributed and not associated with chronological age. Age acceleration was negatively associated with AMH levels (t = -3.1, P = 0.003) and AFC (t = -4.0, P = 0.0001), such that women with a higher age acceleration had a lower ovarian reserve. Age acceleration was also negatively correlated with the total number of oocytes retrieved (t = -3.9, P = 0.0002), the number of mature oocytes (t = -3.8, P = 0.0003) and the number of fertilized oocytes or two-pronuclear oocytes (t = -2.8, P = 0.008) in the main analysis. Limitations, Reasons for Caution: This study used pooled follicular fluid, which does not allow for the investigation of individual follicles. Infertility patients may also be different from the general population, but, as we used granulosa cells, the participants had to be from an IVF population. Wider Implications of the Findings: This study demonstrated that epigenetic age and age acceleration can be calculated from granulosa cells collected at the time of oocyte retrieval. GrimAge most strongly predicted chronological age, and GrimAge acceleration was associated with baseline and cycle characteristics as well as cycle outcomes, which indicates its potential clinical relevance in evaluating both oocyte quantity and quality. Study Funding/competing Interest(s): This study was supported by the National Institutes of Health (UL1TR002378) and the Building Interdisciplinary Research Careers in Women's Health Program (K12HD085850) to A.K.K. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The funding source had no role in any aspect of this study. J.B.S. serves as Vice Chair for the American Society for Reproductive Medicine Education Committee, is a Medical Committee Advisor for the Jewish Fertility Foundation and works with Jscreen. J.B.S. has received funding from Georgia Clinical Translational Research Alliance. H.S.H., J.B.S. and A.K.S. have received NIH funding for other projects. A.K.K., S.A.G., S.G., Q.S.K., L.J.M. and W.S. have no conflicts of interest. Trial Registration Number: N/A. (© The Author(s) 2022. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: journals.permissions@oup.com.) |
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| معلومات مُعتمدة: | K12 HD085850 United States HD NICHD NIH HHS; UL1 TR002378 United States TR NCATS NIH HHS; UL1TR002378 United States NH NIH HHS |
| فهرسة مساهمة: | Keywords: DNA methylation; aging; epigenetic age; follicular fluid; granulosa cells; ovarian reserve |
| المشرفين على المادة: | 80497-65-0 (Anti-Mullerian Hormone) |
| تواريخ الأحداث: | Date Created: 20220809 Date Completed: 20221004 Latest Revision: 20230810 |
| رمز التحديث: | 20230810 |
| مُعرف محوري في PubMed: | PMC9527469 |
| DOI: | 10.1093/humrep/deac178 |
| PMID: | 35944168 |
| قاعدة البيانات: | MEDLINE |
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