دورية أكاديمية
Monocarboxylate transporters (MCTs) in skeletal muscle and hypothalamus of less or more physically active mice exposed to aerobic training.
| العنوان: | Monocarboxylate transporters (MCTs) in skeletal muscle and hypothalamus of less or more physically active mice exposed to aerobic training. |
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| المؤلفون: | Scariot PPM; Laboratory of Applied Sport Physiology, School of Applied Sciences, University of Campinas, Limeira, SP, Brazil., Manchado-Gobatto FB; Laboratory of Applied Sport Physiology, School of Applied Sciences, University of Campinas, Limeira, SP, Brazil., Beck WR; Department of Physiological Sciences, Federal University of São Carlos, SP, Brazil., Papoti M; School of Physical Education and Sport of Ribeirão Preto, University of São Paulo, SP, Brazil., Van Ginkel PR; Department of Genetics & Medical Genetics, University of Wisconsin, Madison, WI, USA., Gobatto CA; Laboratory of Applied Sport Physiology, School of Applied Sciences, University of Campinas, Limeira, SP, Brazil. Electronic address: cgobatto@unicamp.br. |
| المصدر: | Life sciences [Life Sci] 2022 Oct 15; Vol. 307, pp. 120872. Date of Electronic Publication: 2022 Aug 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Monocarboxylic Acid Transporters*/metabolism , Muscle, Skeletal*/metabolism, Animals ; Hypothalamus/metabolism ; Lactic Acid/metabolism ; Male ; Mice ; Mice, Inbred C57BL |
| مستخلص: | Aims: The synthesis of monocarboxylate transporters (MCTs) can be stimulated by aerobic training, but few is known about this effect associated or not with non-voluntary daily activities. We examined the effect of eight weeks of aerobic training in MCTs on the skeletal muscle and hypothalamus of less or more physically active mice, which can be achieved by keeping them in two different housing models, a small cage (SC) and a large cage (LC). Main Methods: Forty male C57BL/6J mice were divided into four groups. In each housing condition, mice were divided into untrained (N) and trained (T). For 8 weeks, the trained animals ran on a treadmill with an intensity equivalent to 80 % of the individual critical velocity (CV), considered aerobic capacity, 40 min/day, 5 times/week. Protein expression of MCTs was determined with fluorescence Western Blot. Key Findings: T groups had higher hypothalamic MCT2 than N groups (ANOVA, P = 0.032). Significant correlations were detected between hypothalamic MCT2 and CV. There was a difference between the SC and LC groups in relation to MCT4 in the hypothalamus (LC > SC, P = 0.044). Trained mice housed in LC (but not SC-T) exhibited a reduction in MCT4 muscle (P < 0.001). Significance: Our findings indicate that aerobically trained mice increased the expression of MCT2 protein in the hypothalamus, which has been related to the uptake of lactate in neurons. Changes in energy metabolism in physically active mice (kept in LC) may be related to upregulation of hypothalamic MCT4, probably participating in the regulation of satiety. Competing Interests: Declaration of competing interest There is no financial/personal interest or belief that could affect their objectivity. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. All authors have seen and approved the final version of the manuscript being submitted. (Copyright © 2022 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Active lifestyle; Aerobic training; C57BL/6 J mice; Critical velocity; Hypothalamus; Monocarboxylate transporters (MCTs); Skeletal muscle |
| المشرفين على المادة: | 0 (Monocarboxylic Acid Transporters) 33X04XA5AT (Lactic Acid) |
| تواريخ الأحداث: | Date Created: 20220810 Date Completed: 20221012 Latest Revision: 20221012 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.lfs.2022.120872 |
| PMID: | 35948119 |
| قاعدة البيانات: | MEDLINE |
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