دورية أكاديمية
Antigen-Dependent Inducible T-Cell Reporter System for PET Imaging of Breast Cancer and Glioblastoma.
| العنوان: | Antigen-Dependent Inducible T-Cell Reporter System for PET Imaging of Breast Cancer and Glioblastoma. |
|---|---|
| المؤلفون: | Shin J; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California., Parker MFL; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California., Zhu I; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California.; Parker Institute for Cancer Immunotherapy, San Francisco, California., Alanizi A; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California., Rodriguez CI; Department of Pathology, University of California, San Francisco, San Francisco, California., Liu R; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California.; Parker Institute for Cancer Immunotherapy, San Francisco, California., Watchmaker PB; Department of Neurological Surgery, University of California, San Francisco, San Francisco, California., Kalita M; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California., Blecha J; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California., Luu J; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California., Wright B; Department of Radiology, University of Alabama at Birmingham, Birmingham, Alabama., Lapi SE; Department of Radiology, University of Alabama at Birmingham, Birmingham, Alabama., Flavell RR; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California.; Helen Diller Cancer Center, University of California, San Francisco, San Francisco, California., Okada H; Parker Institute for Cancer Immunotherapy, San Francisco, California.; Department of Neurological Surgery, University of California, San Francisco, San Francisco, California.; Helen Diller Cancer Center, University of California, San Francisco, San Francisco, California., Tlsty TD; Department of Pathology, University of California, San Francisco, San Francisco, California; david.m.wilson@ucsf.edu kole.roybal@ucsf.edu thea.tlsty@ucsf.edu., Roybal KT; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California; david.m.wilson@ucsf.edu kole.roybal@ucsf.edu thea.tlsty@ucsf.edu.; Parker Institute for Cancer Immunotherapy, San Francisco, California.; Helen Diller Cancer Center, University of California, San Francisco, San Francisco, California.; Chan Zuckerberg Biohub, San Francisco, California.; Gladstone UCSF Institute for Genetic Immunology, San Francisco, California; and.; UCSF Cell Design Institute, San Francisco, California., Wilson DM; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California; david.m.wilson@ucsf.edu kole.roybal@ucsf.edu thea.tlsty@ucsf.edu. |
| المصدر: | Journal of nuclear medicine : official publication, Society of Nuclear Medicine [J Nucl Med] 2023 Jan; Vol. 64 (1), pp. 137-144. Date of Electronic Publication: 2022 Aug 18. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Society of Nuclear Medicine Country of Publication: United States NLM ID: 0217410 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-5667 (Electronic) Linking ISSN: 01615505 NLM ISO Abbreviation: J Nucl Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Reston, VA : Society of Nuclear Medicine Original Publication: [Chicago, Ill.] : S.N. Turiel & Assoc. |
| مواضيع طبية MeSH: | Glioblastoma* , Breast Neoplasms*/diagnostic imaging , Breast Neoplasms*/genetics, Animals ; Humans ; Female ; T-Lymphocytes ; Cell Line, Tumor ; Positron-Emission Tomography/methods ; Genes, Reporter |
| مستخلص: | For the past several decades, chimeric antigen receptor T-cell therapies have shown promise in the treatment of cancers. These treatments would greatly benefit from companion imaging biomarkers to follow the trafficking of T cells in vivo. Methods: Using synthetic biology, we engineered T cells with a chimeric receptor synthetic intramembrane proteolysis receptor (SNIPR) that induces overexpression of an exogenous reporter gene cassette on recognition of specific tumor markers. We then applied a SNIPR-based PET reporter system to 2 cancer-relevant antigens, human epidermal growth factor receptor 2 (HER2) and epidermal growth factor receptor variant III (EGFRvIII), commonly expressed in breast and glial tumors, respectively. Results: Antigen-specific reporter induction of the SNIPR PET T cells was confirmed in vitro using green fluorescent protein fluorescence, luciferase luminescence, and the HSV-TK PET reporter with 9-(4- 18 F-fluoro-3-[hydroxymethyl]butyl)guanine ([ 18 F]FHBG). T cells associated with their target antigens were successfully imaged using PET in dual-xenograft HER2+/HER2- and EGFRvIII+/EGFRvIII- animal models, with more than 10-fold higher [ 18 F]FHBG signals seen in antigen-expressing tumors versus the corresponding controls. Conclusion: The main innovation found in this work was PET detection of T cells via specific antigen-induced signals, in contrast to reporter systems relying on constitutive gene expression. (© 2023 by the Society of Nuclear Medicine and Molecular Imaging.) |
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| معلومات مُعتمدة: | T32 AI007334 United States AI NIAID NIH HHS; R35 NS105068 United States NS NINDS NIH HHS; F30 CA250247 United States CA NCI NIH HHS; R01 AI161829 United States AI NIAID NIH HHS; R01 EB024014 United States EB NIBIB NIH HHS; R01 EB025985 United States EB NIBIB NIH HHS; R01 EB030897 United States EB NIBIB NIH HHS |
| فهرسة مساهمة: | Keywords: CAR T; PET; SNIPR; cancer antigens; reporter |
| تواريخ الأحداث: | Date Created: 20220818 Date Completed: 20230106 Latest Revision: 20230910 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9841254 |
| DOI: | 10.2967/jnumed.122.264284 |
| PMID: | 35981900 |
| قاعدة البيانات: | MEDLINE |
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