دورية أكاديمية
Vasculogenesis in kidney organoids upon transplantation.
| العنوان: | Vasculogenesis in kidney organoids upon transplantation. |
|---|---|
| المؤلفون: | Koning M; Department of Internal Medicine - Nephrology, Leiden University Medical Center, Leiden, the Netherlands.; Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, the Netherlands., Dumas SJ; Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, VIB Center for Cancer Biology, VIB, Leuven, 3000, Belgium., Avramut MC; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, the Netherlands., Koning RI; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, the Netherlands., Meta E; Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, VIB Center for Cancer Biology, VIB, Leuven, 3000, Belgium., Lievers E; Department of Internal Medicine - Nephrology, Leiden University Medical Center, Leiden, the Netherlands.; Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, the Netherlands., Wiersma LE; Department of Internal Medicine - Nephrology, Leiden University Medical Center, Leiden, the Netherlands.; Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, the Netherlands., Borri M; Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, VIB Center for Cancer Biology, VIB, Leuven, 3000, Belgium., Liang X; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen, Qingdao, China., Xie L; MGI, BGI-Shenzhen, Shenzhen, China., Liu P; MGI, BGI-Shenzhen, Shenzhen, China., Chen F; MGI, BGI-Shenzhen, Shenzhen, China., Lin L; Department of Biomedicine, Aarhus University, Aarhus, Denmark.; Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark., Luo Y; Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, BGI-Shenzhen, Qingdao, China.; Department of Biomedicine, Aarhus University, Aarhus, Denmark.; Steno Diabetes Center Aarhus, Aarhus University Hospital, Aarhus, Denmark., Mulder J; Department of Pediatrics, Leiden University Medical Center, Leiden, the Netherlands.; Division of Nephrology, Department of Pediatrics, Erasmus Medical Center, Rotterdam, the Netherlands., Spijker HS; Department of Internal Medicine - Nephrology, Leiden University Medical Center, Leiden, the Netherlands.; Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, the Netherlands., Jaffredo T; Sorbonne Université, IBPS, CNRS UMR7622, Inserm U 1156, Developmental Biology Laboratory, Paris, France., van den Berg BM; Department of Internal Medicine - Nephrology, Leiden University Medical Center, Leiden, the Netherlands.; Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, the Netherlands., Carmeliet P; Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology and Leuven Cancer Institute (LKI), KU Leuven, VIB Center for Cancer Biology, VIB, Leuven, 3000, Belgium.; Laboratory of Angiogenesis and Vascular Heterogeneity, Department of Biomedicine, Aarhus University, Aarhus, 8000, Denmark.; State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong, People's Republic of China., van den Berg CW; Department of Internal Medicine - Nephrology, Leiden University Medical Center, Leiden, the Netherlands.; Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, the Netherlands.; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, Leiden, the Netherlands., Rabelink TJ; Department of Internal Medicine - Nephrology, Leiden University Medical Center, Leiden, the Netherlands. a.j.rabelink@lumc.nl.; Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, the Netherlands. a.j.rabelink@lumc.nl.; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, Leiden, the Netherlands. a.j.rabelink@lumc.nl. |
| المصدر: | NPJ Regenerative medicine [NPJ Regen Med] 2022 Aug 19; Vol. 7 (1), pp. 40. Date of Electronic Publication: 2022 Aug 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group, published in partnership with Monash University and Australian Regenerative Medicine Institute Country of Publication: United States NLM ID: 101699846 Publication Model: Electronic Cited Medium: Internet ISSN: 2057-3995 (Electronic) Linking ISSN: 20573995 NLM ISO Abbreviation: NPJ Regen Med Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [New York] : Nature Publishing Group, published in partnership with Monash University and Australian Regenerative Medicine Institute, [2016]- |
| مستخلص: | Human induced pluripotent stem cell-derived kidney organoids have potential for disease modeling and to be developed into clinically transplantable auxiliary tissue. However, they lack a functional vasculature, and the sparse endogenous endothelial cells (ECs) are lost upon prolonged culture in vitro, limiting maturation and applicability. Here, we use intracoelomic transplantation in chicken embryos followed by single-cell RNA sequencing and advanced imaging platforms to induce and study vasculogenesis in kidney organoids. We show expansion of human organoid-derived ECs that reorganize into perfused capillaries and form a chimeric vascular network with host-derived blood vessels. Ligand-receptor analysis infers extensive potential interactions of human ECs with perivascular cells upon transplantation, enabling vessel wall stabilization. Perfused glomeruli display maturation and morphogenesis to capillary loop stage. Our findings demonstrate the beneficial effect of vascularization on not only epithelial cell types, but also the mesenchymal compartment, inducing the expansion of ´on target´ perivascular stromal cells, which in turn are required for further maturation and stabilization of the neo-vasculature. The here described vasculogenic capacity of kidney organoids will have to be deployed to achieve meaningful glomerular maturation and kidney morphogenesis in vitro. (© 2022. The Author(s).) |
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| معلومات مُعتمدة: | 846615 EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 Marie Skłodowska-Curie Actions (H2020 Excellent Science - Marie Skłodowska-Curie Actions) |
| تواريخ الأحداث: | Date Created: 20220819 Latest Revision: 20220826 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC9391397 |
| DOI: | 10.1038/s41536-022-00237-4 |
| PMID: | 35986027 |
| قاعدة البيانات: | MEDLINE |
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