دورية أكاديمية
أعرض في EDS

Differentiation of equine mesenchymal stem cells into cells of osteochondral lineage: potential for osteochondral tissue engineering.

التفاصيل البيبلوغرافية
العنوان: Differentiation of equine mesenchymal stem cells into cells of osteochondral lineage: potential for osteochondral tissue engineering.
المؤلفون: Cheng HW; Tissue Engineering Laboratory, Biomedical Engineering Programme, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong Special Administrative Region of China., Chik TK; Living Tissues Company Limited, Unit 620, Biotech Centre 2, No. 11 Science Park West Avenue, Hong Kong Science Park, Pak Shek Kok, Hong Kong., Weir J; Department of Veterinary Clinical Services, The Hong Kong Jockey Club, Sha Tin Racecourse, Shatin, Hong Kong Special Administrative Region of China., Chan BP; Tissue Engineering Laboratory, Biomedical Engineering Programme, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong Special Administrative Region of China.
المصدر: Biomedical materials (Bristol, England) [Biomed Mater] 2022 Sep 06; Vol. 17 (6). Date of Electronic Publication: 2022 Sep 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Institute of Physics Pub Country of Publication: England NLM ID: 101285195 Publication Model: Electronic Cited Medium: Internet ISSN: 1748-605X (Electronic) Linking ISSN: 17486041 NLM ISO Abbreviation: Biomed Mater Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Bristol, UK : Institute of Physics Pub.,
مواضيع طبية MeSH: Cartilage, Articular* , Mesenchymal Stem Cells*, Animals ; Cell Differentiation ; Chondrocytes ; Chondrogenesis ; Collagen/metabolism ; Horses ; Humans ; Tissue Engineering/methods ; Tissue Scaffolds
مستخلص: Damage to the hyaline cartilage of the joint surface and osteochondral fractures are key factors leading to the development of osteoarthritis in racehorses, representing a significant cause of racehorse retirement. To tissue-engineer an osteochondral unit that is suitable for joint repair, incorporation of a zone of calcified cartilage should be considered so as to mimic its in vivo counterpart. To date, equine mesenchymal stem cells (eMSCs) have been reported to have multilineage differentiation potential. Yet the generation of a zone of calcified cartilage using eMSCs has not been reported. This work is an initial attempt to generate a zone of calcified cartilage using eMSCs as the single source of cells and collagen as the scaffolding material. Main advantages of using eMSCs over equine deep zone chondrocytes for the generation of a zone of calcified cartilage include no donor site morbidity and their ease of expansion in culture. Initially, we fabricated cartilage-like tissues and bone-like tissues in vitro by differentiating eMSCs toward chondrogenic and osteogenic lineages for 21 d, respectively. We then aggregated the cartilage-like and bone-like tissues together with a layer of undifferentiated eMSCs-collagen gel in between to generate a 3-layer osteochondral unit. A zone of calcified cartilage was found between the cartilage-like and bone-like layers after a 14-day culture in chondrogenic differentiation medium. These results provide a solution toward tissue engineering of equine osteochondral units with interfacial zone without using chondrocytes harvested from the deep zone of healthy articular cartilage, and contribute to the future development of osteochondral tissue engineering strategies for human cartilage injuries in the long run.
(© 2022 IOP Publishing Ltd.)
فهرسة مساهمة: Keywords: chondrogenic; equine mesenchymal stem cells; osteochondral; osteogenic; tissue engineering
المشرفين على المادة: 9007-34-5 (Collagen)
تواريخ الأحداث: Date Created: 20220824 Date Completed: 20220908 Latest Revision: 20220923
رمز التحديث: 20231215
DOI: 10.1088/1748-605X/ac8c76
PMID: 36001983
قاعدة البيانات: MEDLINE
الوصف
تدمد:1748-605X
DOI:10.1088/1748-605X/ac8c76