دورية أكاديمية
أعرض في EDS

Carbohydrate intake in recovery from aerobic exercise differentiates skeletal muscle microRNA expression.

التفاصيل البيبلوغرافية
العنوان: Carbohydrate intake in recovery from aerobic exercise differentiates skeletal muscle microRNA expression.
المؤلفون: Margolis LM; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts., Carrigan CT; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts., Murphy NE; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts., DiBella MN; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts.; Oak Ridge Institute of Science and Education, Belcamp, Maryland., Wilson MA; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts., Whitney CC; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts., Howard EE; Military Nutrition Division, U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts., Pasiakos SM; Military Performance Division, U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts., Rivas DA; Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts.
المصدر: American journal of physiology. Endocrinology and metabolism [Am J Physiol Endocrinol Metab] 2022 Nov 01; Vol. 323 (5), pp. E435-E447. Date of Electronic Publication: 2022 Aug 31.
نوع المنشور: Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1555 (Electronic) Linking ISSN: 01931849 NLM ISO Abbreviation: Am J Physiol Endocrinol Metab Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Bethesda, MD. : American Physiological Society
مواضيع طبية MeSH: Glycogen*/metabolism , MicroRNAs*/genetics , MicroRNAs*/metabolism, Adolescent ; Adult ; Humans ; Male ; Young Adult ; Dietary Carbohydrates/pharmacology ; Dietary Carbohydrates/metabolism ; Exercise/physiology ; Muscle, Skeletal/metabolism
مستخلص: Posttranscriptional regulation by microRNA (miRNA) facilitates exercise and diet-induced skeletal muscle adaptations. However, the impact of diet on miRNA expression during postexercise recovery remains unclear. The objective of this study was to examine the effects of consuming carbohydrate or a nutrient-free control on skeletal muscle miRNA expression during 3 h of recovery from aerobic exercise. Using a randomized, crossover design, seven men (means ± SD, age: 21 ± 3 yr; body mass: 83 ± 13 kg; V̇o 2peak : 43 ± 2 mL/kg/min) completed two-cycle ergometry glycogen depletion trials followed by 3 h of recovery while consuming either carbohydrate (CHO: 1 g/kg/h) or control (CON: nutrient free). Muscle biopsy samples were obtained under resting fasted conditions at baseline and at the end of the 3-h recovery (REC) period. miRNA expression was determined using unbiased RT-qPCR microarray analysis. Trials were separated by 7 days. Twenty-five miRNAs were different ( P < 0.05) between CHO and CON at REC, with Let7i-5p and miR-195-5p being the most predictive of treatment. In vitro overexpression of Let7i-5p and miR-195-p5 in C2C12 skeletal muscle cells decreased ( P < 0.05) the expression of protein breakdown ( Foxo1 , Trim63 , Casp3 , and Atf4 ) genes, ubiquitylation, and protease enzyme activity compared with control. Energy sensing ( Prkaa1 and Prkab1 ) and glycolysis ( Gsy1 and Gsk3b ) genes were lower ( P < 0.05) with Let7i-5p overexpression compared with miR-195-5p and control. Fat metabolism ( Cpt1a , Scd1 , and Hadha ) genes were lower ( P < 0.05) in miR-195-5p than in control. These data indicate that consuming CHO after aerobic exercise alters miRNA profiles compared with CON, and these differences may govern mechanisms facilitating muscle recovery. NEW & NOTEWORTHY Results provide novel insight into effects of carbohydrate intake on the expression of skeletal muscle microRNA during early recovery from aerobic exercise and reveal that Let7i-5p and miR-195-5p are important regulators of skeletal muscle protein breakdown to aid in facilitating muscle recovery.
References: Diabetes. 2007 Oct;56(10):2449-56. (PMID: 17623817)
Front Physiol. 2016 Mar 07;7:87. (PMID: 27014087)
Oncol Lett. 2020 Sep;20(3):2613-2620. (PMID: 32782579)
Nucleic Acids Res. 2013 Sep;41(16):7683-99. (PMID: 23804767)
Am J Physiol Cell Physiol. 2021 Dec 1;321(6):C977-C991. (PMID: 34705586)
Mol Nutr Food Res. 2018 Apr;62(7):e1701028. (PMID: 29377592)
Am J Physiol Endocrinol Metab. 2018 Oct 1;315(4):E723-E733. (PMID: 29969318)
Nat Commun. 2014 Oct 10;5:5212. (PMID: 25301052)
J Physiol. 2022 Sep;600(17):3951-3963. (PMID: 35822542)
Tumour Biol. 2017 Jun;39(6):1010428317711324. (PMID: 28639885)
J Biol Chem. 2012 Jul 27;287(31):25727-40. (PMID: 22669940)
Int J Sport Nutr Exerc Metab. 2004 Jun;14(3):255-71. (PMID: 15256687)
Am J Physiol Cell Physiol. 2009 Jun;296(6):C1248-57. (PMID: 19357234)
Pharmacol Ther. 2015 Apr;148:114-31. (PMID: 25435019)
Metabolism. 2019 Aug;97:1-8. (PMID: 31095946)
Med Sci Sports Exerc. 2005 Mar;37(3):404-11. (PMID: 15741838)
Am J Physiol Endocrinol Metab. 2008 Sep;295(3):E595-604. (PMID: 18577697)
Nat Metab. 2020 Sep;2(9):817-828. (PMID: 32747792)
FASEB J. 2022 Feb;36(2):e22152. (PMID: 35061305)
Cell Metab. 2021 Apr 6;33(4):758-780. (PMID: 33826918)
Proc Natl Acad Sci U S A. 2015 May 26;112(21):E2785-94. (PMID: 25964336)
Med Sci Sports Exerc. 2016 Mar;48(3):543-68. (PMID: 26891166)
J Appl Physiol (1985). 2019 Jun 1;126(6):1587-1597. (PMID: 31046515)
Int J Mol Sci. 2021 Dec 22;23(1):. (PMID: 35008539)
Nat Commun. 2015 Apr 10;6:6670. (PMID: 25858807)
Front Physiol. 2017 Mar 23;8:182. (PMID: 28386239)
Nature. 2011 Jan 20;469(7330):336-42. (PMID: 21248840)
Am J Physiol Endocrinol Metab. 2014 Sep 15;307(6):E469-84. (PMID: 25096180)
Curr Dev Nutr. 2017 May 23;1(6):e000893. (PMID: 29955707)
J Nutr. 2009 Dec;139(12):2279-84. (PMID: 19828686)
J Appl Physiol (1985). 2011 Mar;110(3):834-45. (PMID: 21030665)
Nucleic Acids Res. 2001 May 1;29(9):e45. (PMID: 11328886)
Nucleic Acids Res. 2014 Dec 16;42(22):13799-811. (PMID: 25399420)
Cell. 2004 Apr 30;117(3):399-412. (PMID: 15109499)
Arch Physiol Biochem. 2009 Feb;115(1):13-21. (PMID: 19267278)
Prog Mol Biol Transl Sci. 2015;135:17-37. (PMID: 26477909)
FASEB J. 2011 Mar;25(3):1028-39. (PMID: 21148113)
Cell. 2017 Apr 20;169(3):381-405. (PMID: 28431241)
Am J Physiol Cell Physiol. 2006 Feb;290(2):C453-62. (PMID: 16162663)
Med Sci Sports Exerc. 2021 Aug 1;53(8):1708-1718. (PMID: 33731656)
Am J Physiol Regul Integr Comp Physiol. 2010 Aug;299(2):R533-40. (PMID: 20519362)
Science. 2001 Nov 23;294(5547):1704-8. (PMID: 11679633)
J Physiol. 2013 Sep 15;591(18):4637-53. (PMID: 23798494)
Exerc Sport Sci Rev. 2017 Jul;45(3):187-191. (PMID: 28419002)
J Clin Invest. 2004 Jan;113(1):115-23. (PMID: 14702115)
PLoS One. 2016 Jan 19;11(1):e0146726. (PMID: 26785133)
FASEB J. 2013 Dec;27(12):5104-11. (PMID: 23965841)
Nat Commun. 2021 Oct 12;12(1):5948. (PMID: 34642330)
Physiol Rep. 2018 Dec;6(23):e13931. (PMID: 30548426)
معلومات مُعتمدة: K01 AG047247 United States AG NIA NIH HHS; P30 AG031679 United States AG NIA NIH HHS
فهرسة مساهمة: Keywords: Let7; endurance exercise; glycogen; miR-195
المشرفين على المادة: 0 (Dietary Carbohydrates)
9005-79-2 (Glycogen)
0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20220831 Date Completed: 20221115 Latest Revision: 20231102
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9639755
DOI: 10.1152/ajpendo.00110.2022
PMID: 36044708
قاعدة البيانات: MEDLINE
الوصف
تدمد:1522-1555
DOI:10.1152/ajpendo.00110.2022