دورية أكاديمية
أعرض في EDS

Genotypic characterization of vancomycin-resistant Enterococcus causing urinary tract infection in northern India.

التفاصيل البيبلوغرافية
العنوان: Genotypic characterization of vancomycin-resistant Enterococcus causing urinary tract infection in northern India.
المؤلفون: Das AK; Department of Microbiology, Hamdard Institute of Medical Sciences and Research & Hakeem Abdul Hamid Centenary Hospital, Jamia Hamdard, New Delhi, India., Dudeja M; Department of Microbiology, Hamdard Institute of Medical Sciences and Research & Hakeem Abdul Hamid Centenary Hospital, Jamia Hamdard, New Delhi, India., Kohli S; Department of Medicine, Hamdard Institute of Medical Sciences and Research & Hakeem Abdul Hamid Centenary Hospital, Jamia Hamdard, New Delhi, India., Ray P; Department of Biotechnology, School of Chemical & Life Sciences, Jamia Hamdard, New Delhi, India.
المصدر: The Indian journal of medical research [Indian J Med Res] 2022 Mar; Vol. 155 (3&4), pp. 423-431.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Medknow Country of Publication: India NLM ID: 0374701 Publication Model: Print Cited Medium: Internet ISSN: 0971-5916 (Print) Linking ISSN: 09715916 NLM ISO Abbreviation: Indian J Med Res Subsets: MEDLINE
أسماء مطبوعة: Publication: <2011- > : Mumbai : Medknow
Original Publication: New Delhi : Council Of Medical Research
مواضيع طبية MeSH: Urinary Tract Infections*/drug therapy , Urinary Tract Infections*/epidemiology , Urinary Tract Infections*/genetics , Vancomycin-Resistant Enterococci*, Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Cross-Sectional Studies ; Erythromycin ; Fluoroquinolones ; Genotype ; Gentamicins ; Humans ; Linezolid/therapeutic use ; Pristinamycin ; Teicoplanin ; Tigecycline ; Vancomycin/therapeutic use ; beta-Lactams
مستخلص: Background & Objectives: Infections caused by vancomycin-resistant Enterococci are difficult to treat given the limited therapeutic alternatives. Different gene clusters are known to confer vancomycin resistance. vanA and vanB genes are transferable and are clinically relevant. This cross-sectional study aimed to identify the vancomycin-resistant genotypes in the strains causing urinary tract infection and also to test the in vitro efficacy of linezolid and pristinamycin against the vancomycin-resistant isolates.
Methods: Antimicrobial resistance profile of 118 enterococcal isolates was evaluated. Minimum inhibitory concentration of vancomycin, teicoplanin and high-level gentamicin (HLG) was determined by micro broth dilution. The vancomycin-resistant isolates were tested against linezolid and pristinamycin by micro-broth dilution and E strip method. The presence of vancomycin-resistant genes was detected by multiplex polymerase chain reaction and was sequenced and analyzed.
Results: Most commonly isolated species were Enterococcus faecalis (76.9%) and Enterococcus faecium (16.9%). It was found that 43 per cent of the isolates were resistant to HLG and 16.9 per cent to vancomycin. Higher resistance was seen against fluoroquinolones, erythromycin, tetracycline and β-lactam drugs. However, 5.08 per cent strains were resistant to tigecycline. All vancomycin-resistant strains were sensitive to pristinamycin and one was resistant to linezolid. vanA and vanB gene were found in 15 and five isolates, respectively. The gene sequences were submitted to NCBI gene bank and accession numbers were obtained.
Interpretation & Conclusions: The present study showed prevalence of vanA and vanB genes carrying Enterococcus in a tertiary care centre in north India. The emergence of resistance against drugs such as tigecycline and linezolid is a topic of concern as it will be a therapeutic challenge for physicians.
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فهرسة مساهمة: Keywords: Enterococcus; linezolid –; resistance –; tigecyline; vanA; vanB; vancomycin
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Fluoroquinolones)
0 (Gentamicins)
0 (Pristinamycin)
0 (beta-Lactams)
61036-62-2 (Teicoplanin)
63937KV33D (Erythromycin)
6Q205EH1VU (Vancomycin)
70JE2N95KR (Tigecycline)
ISQ9I6J12J (Linezolid)
تواريخ الأحداث: Date Created: 20220920 Date Completed: 20220922 Latest Revision: 20221202
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9707681
DOI: 10.4103/ijmr.IJMR_2554_19
PMID: 36124515
قاعدة البيانات: MEDLINE
الوصف
تدمد:0971-5916
DOI:10.4103/ijmr.IJMR_2554_19