دورية أكاديمية
A pilot biomonitoring study of air pollution in the urban area of Sarajevo, Bosnia and Herzegovina: genotoxicity assessment in buccal cells.
| العنوان: | A pilot biomonitoring study of air pollution in the urban area of Sarajevo, Bosnia and Herzegovina: genotoxicity assessment in buccal cells. |
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| المؤلفون: | Cetkovic T; Laboratory for Cytogenetics and Genotoxicology, University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina., Haveric A; Laboratory for Cytogenetics and Genotoxicology, University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina., Behmen S; Section for Toxicology and Mutagenesis, Genetic Association in Bosnia and Herzegovina, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina., Hadzic Omanovic M; Laboratory for Cytogenetics and Genotoxicology, University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina., Caluk Klacar L; Laboratory for Cytogenetics and Genotoxicology, University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina., Dzaferspahic A; Section for Toxicology and Mutagenesis, Genetic Association in Bosnia and Herzegovina, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina., Durmisevic I; Section for Toxicology and Mutagenesis, Genetic Association in Bosnia and Herzegovina, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina., Mehanovic M; Section for Toxicology and Mutagenesis, Genetic Association in Bosnia and Herzegovina, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina., Haveric S; Laboratory for Cytogenetics and Genotoxicology, University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja od Bosne 8, Sarajevo 71000, Bosnia and Herzegovina. |
| المصدر: | Mutagenesis [Mutagenesis] 2023 Feb 03; Vol. 38 (1), pp. 33-42. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8707812 Publication Model: Print Cited Medium: Internet ISSN: 1464-3804 (Electronic) Linking ISSN: 02678357 NLM ISO Abbreviation: Mutagenesis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1995- : Swansea, UK : Oxford University Press Original Publication: Oxford ; Washington, DC : Published for the United Kingdom Environmental Mutagen Society by IRL Press, [1986- |
| مواضيع طبية MeSH: | Biological Monitoring* , Air Pollution*/adverse effects, Humans ; Bosnia and Herzegovina ; Mouth Mucosa ; DNA Damage ; Comet Assay ; Micronucleus Tests/methods |
| مستخلص: | Air pollution, recognized as a human carcinogen, is a significant cause of death in industrial and developing countries, and Bosnia and Herzegovina (B&H) is one of the leading countries for air pollution-caused death rate and has the poorest urban air quality in Europe. Despite a population decrease, urban air pollution in B&H has increased due to traffic pollution and still intensive use of solid fuel for heating and cooking. Human biomonitoring studies, regarding the described air pollution, have not been conducted before, and particularly have not been conducted in the region of Sarajevo. Good health, well-being, and environmental protection are part of the 17 defined Sustainable Development Global Goals. Accordingly, this study aimed to determine baseline levels of DNA damage in a group of Sarajevo citizens and to compare seasonal variations in DNA damage in relation to the reported levels of air pollution. From 33 individuals included in the study, samples were collected in the summer and winter seasons. The buccal micronucleus cytome (BMCyt) assay and comet assay in leucocytes isolated from saliva were performed. Mean values and standard deviations of log-transformed tail intensity (%), tail length (µm), and tail moment results in winter were 1.14 ± 0.23, 2.20 ± 0.14, and 1.03 ± 0.29, respectively, while in the summer season those values were 1.19 ± 0.19, 2.25 ± 0.17, and 1.07 ± 0.25, respectively. No significant differences were found for the comet assay parameters. Nevertheless, BMCyt results showed significant increases in micronuclei (P = .008), binuclear cells (P = .04), karyolysis (P = .0003), condensed chromatin (P = .03), and pyknosis (P = .002) in winter. Although the results of comet and BMCyt assays are not in accordance, this study contributes to the human air pollution biomonitoring in Sarajevo, B&H, and based on the genotoxic effects of air pollution evidenced by the BMCyt biomarker further studies of this kind are necessary. (© The Author(s) 2022. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| فهرسة مساهمة: | Keywords: buccal micronucleus cytome assay; buccal mucosa cells; comet assay; oral leucocytes |
| تواريخ الأحداث: | Date Created: 20220920 Date Completed: 20230207 Latest Revision: 20230215 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1093/mutage/geac016 |
| PMID: | 36125092 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1464-3804 |
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| DOI: | 10.1093/mutage/geac016 |