دورية أكاديمية
Rv0233 is not essential for the survival of Mycobacterium tuberculosis in stress conditions.
| العنوان: | Rv0233 is not essential for the survival of Mycobacterium tuberculosis in stress conditions. |
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| المؤلفون: | Ikeh MA; Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK., Parish T; Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA. |
| المصدر: | Microbiology (Reading, England) [Microbiology (Reading)] 2022 Sep; Vol. 168 (9). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 9430468 Publication Model: Print Cited Medium: Internet ISSN: 1465-2080 (Electronic) Linking ISSN: 13500872 NLM ISO Abbreviation: Microbiology (Reading) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, U.K. : Society for General Microbiology, c1994- |
| مواضيع طبية MeSH: | Mycobacterium tuberculosis*/genetics , Ribonucleotide Reductases*, Hydrogen Peroxide/pharmacology ; Macrophages/microbiology ; Nitrites |
| مستخلص: | Mycobacterium tuberculosis is an important global pathogen. We were interested in understanding the role of Rv0233, a proposed subunit of the class IB ribonucleotide reductase, and its role in surviving stress conditions. We constructed an in-frame, unmarked deletion strain of M. tuberculosis and characterized its growth and survival under replicating or non-replicating conditions. We confirmed previous studies that found that Rv0233 is not essential for aerobic growth or survival in the presence of nitrite. We demonstrated that the deletion of Rv0233 does not affect susceptibility to frontline tuberculosis drugs or hydrogen peroxide. The deletion strain survived equally well under nutrient starvation or in hypoxia and was not attenuated for growth in macrophages. |
| فهرسة مساهمة: | Keywords: Mycobacterium tuberculosis; Rv0233; essentiality; macrophage infection; non-replicating; ribonucleotide reductase; stress conditions |
| المشرفين على المادة: | 0 (Nitrites) BBX060AN9V (Hydrogen Peroxide) EC 1.17.4.- (Ribonucleotide Reductases) |
| تواريخ الأحداث: | Date Created: 20220923 Date Completed: 20220928 Latest Revision: 20221005 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1099/mic.0.001199 |
| PMID: | 36149732 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2080 |
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| DOI: | 10.1099/mic.0.001199 |