دورية أكاديمية
In vitro evaluation of the antibacterial and cytotoxic activities of the Euclea natalensis crude extract and fractions against oral infection agents.
| العنوان: | In vitro evaluation of the antibacterial and cytotoxic activities of the Euclea natalensis crude extract and fractions against oral infection agents. |
|---|---|
| المؤلفون: | Lima DDC; Faculty of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Pitorro TEA; Faculty of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Santiago MB; Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Franco RR; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Silva TDC; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Prado DG; Institute of Chemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Cunha LCS; Chemistry Department, Federal Institute of Triângulo Mineiro, Uberaba, Minas Gerais, Brazil., Espindola FS; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Tavares DC; Mutagenesis Laboratory, University of Franca, Franca, São Paulo, Brazil., Nicolella HD; Mutagenesis Laboratory, University of Franca, Franca, São Paulo, Brazil., Martins CHG; Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil. Electronic address: carlos.martins2@ufu.br., Novais VR; Faculty of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil. Electronic address: veridiana.novais@ufu.br. |
| المصدر: | Archives of oral biology [Arch Oral Biol] 2022 Nov; Vol. 143, pp. 105546. Date of Electronic Publication: 2022 Sep 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 0116711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1506 (Electronic) Linking ISSN: 00039969 NLM ISO Abbreviation: Arch Oral Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Pergamon Press Original Publication: Oxford, New York [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Ebenaceae* , Lignans* , Naphthoquinones*/pharmacology, Anti-Bacterial Agents/chemistry ; Bacteria ; Coumarins ; Fatty Acids ; Humans ; Microbial Sensitivity Tests ; Plant Extracts/chemistry |
| مستخلص: | Objective: This study aimed (i) to evaluate the antibacterial and cytotoxic activities of the crude extract and fractions obtained from Euclea natalensis A.D.C. roots against bacteria that cause periodontal disease and caries and (ii) to identify the isolated compounds. Design: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the extract and fractions were determined by the microplate dilution assay. The cytotoxicity of the extract and fractions was evaluated by using the XTT colorimetric assay and normal human fibroblast cells (GM07492A, lung fibroblasts). The compounds present in the most promising fraction were determined by qualitative analysis through liquid chromatography coupled to mass spectrometry (HPLC-MS-ESI). Results: The MIC results ranged from 25 to > 400 μg/mL for the extract and from 1.56 to > 400 μg/mL for the fractions. To evaluate cytotoxicity, the tested concentrations of the extract and fractions ranged from 19.5 to 2500 μg/mL; IC Conclusions: The E. natalensis root extract and fractions displayed good antibacterial activity against periodontal pathogenic and cariogenic bacteria. The antibacterial activity may be due to compounds present in the extract and fractions, which also showed low cytotoxicity to normal human cells. These data are relevant and encourage further research into this plant species, which may contribute to the discovery of new herbal medicines that will help to mitigate the problems caused by oral pathogenic bacteria. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibacterial activity; Cytotoxic activity; Euclea Natalensis; Medicinal plants; Oral microorganisms |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Coumarins) 0 (Fatty Acids) 0 (Lignans) 0 (Naphthoquinones) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20220926 Date Completed: 20221005 Latest Revision: 20221005 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.archoralbio.2022.105546 |
| PMID: | 36162339 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1879-1506 |
|---|---|
| DOI: | 10.1016/j.archoralbio.2022.105546 |