دورية أكاديمية
Screening for Taylorella equigenitalis in Equine Semen: An Exploratory Study.
| العنوان: | Screening for Taylorella equigenitalis in Equine Semen: An Exploratory Study. |
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| المؤلفون: | Mawhinney I; APHA Veterinary Investigation Centre, Rougham Hill, Bury St Edmunds, Suffolk, UK. Electronic address: ian.mawhinney@apha.gov.uk., Davis N; APHA Veterinary Investigation Centre, Rougham Hill, Bury St Edmunds, Suffolk, UK., Carson T; APHA Veterinary Investigation Centre, Rougham Hill, Bury St Edmunds, Suffolk, UK., Torrens N; APHA Veterinary Investigation Centre, Merrythought, Calthwaite, Penrith, Cumbria, UK., Wales A; Department of Pathology and Infectious Diseases, School of Veterinary Medicine, University of Surrey, Guildford, UK. Electronic address: a.wales@surrey.ac.uk. |
| المصدر: | Journal of equine veterinary science [J Equine Vet Sci] 2022 Dec; Vol. 119, pp. 104138. Date of Electronic Publication: 2022 Oct 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 8216840 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0737-0806 (Print) Linking ISSN: 07370806 NLM ISO Abbreviation: J Equine Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003- : New York : Elsevier Original Publication: [Wildomar, CA : William E. Jones, c1981- |
| مواضيع طبية MeSH: | Taylorella equigenitalis* , Horse Diseases*/diagnosis , Gram-Negative Bacterial Infections*/diagnosis , Gram-Negative Bacterial Infections*/veterinary, Horses ; Animals ; Male ; Reproducibility of Results ; Semen |
| مستخلص: | The study examined and compared the sensitivity of culture and a quantitative PCR assay for screening equine semen for the presence of Taylorella equigenitalis (CEMO). Chilled semen samples, both raw and treated with extender, from two stallions were spiked with the organism at seven or 23 days postejaculation and prepared in serial dilutions. Culture of the 7-day raw semen readily detected CEMO at all dilutions, but extended semen yielded counts that were two log cycles lower at equivalent dilutions, with the organism being nearly undetectable at the maximal dilutions. By contrast, PCR sensitivity was not affected by extender, but for 7-day-old raw semen, PCR detection declined abruptly three log dilutions earlier than detection by culture. The more aged 23-day-old semen proved less satisfactory for spiking, with detection of CEMO by culture failing in three of the four samples due to overgrowth with commensal organisms. However, PCR performance was similar in both the 23- and 7-day spiking series. The detection limit by PCR is estimated at between 10 4 and 10 5 cfu/mL. Typical CEMO concentrations in the semen of colonized stallions are not widely reported but where natural semen contamination has been investigated, the organism was present at this order of magnitude. The reliability of detecting CEMO infection using semen samples by either method is discussed. (Crown Copyright © 2022. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Contagious equine metritis; Culture; PCR; Semen; Taylorella equigenitalis |
| تواريخ الأحداث: | Date Created: 20221016 Date Completed: 20221216 Latest Revision: 20221221 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.jevs.2022.104138 |
| PMID: | 36244608 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0737-0806 |
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| DOI: | 10.1016/j.jevs.2022.104138 |