دورية أكاديمية
Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy.
| العنوان: | Effects of Dermatan Sulfate from Marine Invertebrate Styela plicata in the Wound Healing Pathway: A Natural Resource Applied to Regenerative Therapy. |
|---|---|
| المؤلفون: | Rizzo-Valente VS; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil.; Laboratory of Biochemistry and Cell Biology of Glycoconjugates, Clementino Fraga Filho University Hospital and Institute of Medical Biochemistry Leopoldo De Meis, Federal University of Rio de Janeiro, Rio de Janeiro 21941-913, Brazil., Fusco MA; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Cruz RMML; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Santos RA; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Silva LS; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Escaleira RC; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Schulz DF; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Barroso SPC; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Miranda BL; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Santos DZ; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Gregório ML; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Guerra RJA; Biomedical Research Institute, Marcílio Dias Naval Hospital, Brazilian Navy, Rio de Janeiro 20725-090, Brazil., Pavão MSG; Laboratory of Biochemistry and Cell Biology of Glycoconjugates, Clementino Fraga Filho University Hospital and Institute of Medical Biochemistry Leopoldo De Meis, Federal University of Rio de Janeiro, Rio de Janeiro 21941-913, Brazil. |
| المصدر: | Marine drugs [Mar Drugs] 2022 Oct 28; Vol. 20 (11). Date of Electronic Publication: 2022 Oct 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101213729 Publication Model: Electronic Cited Medium: Internet ISSN: 1660-3397 (Electronic) Linking ISSN: 16603397 NLM ISO Abbreviation: Mar Drugs Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2003]- |
| مواضيع طبية MeSH: | Dermatan Sulfate*/metabolism , Dermatan Sulfate*/pharmacology , Urochordata*/metabolism, Animals ; Mice ; Mice, Inbred C57BL ; Wound Healing ; Natural Resources |
| مستخلص: | Acute and chronic dermatological injuries need rapid tissue repair due to the susceptibility to infections. To effectively promote cutaneous wound recovery, it is essential to develop safe, low-cost, and affordable regenerative tools. Therefore, we aimed to identify the biological mechanisms involved in the wound healing properties of the glycosaminoglycan dermatan sulfate (DS), obtained from ascidian Styela plicata , a marine invertebrate, which in preliminary work from our group showed no toxicity and promoted a remarkable fibroblast proliferation and migration. In this study, 2,4-DS (50 µg/mL)-treated and control groups had the relative gene expression of 84 genes participating in the healing pathway evaluated. The results showed that 57% of the genes were overexpressed during treatment, 16% were underexpressed, and 9.52% were not detected. In silico analysis of metabolic interactions exhibited overexpression of genes related to: extracellular matrix organization, hemostasis, secretion of inflammatory mediators, and regulation of insulin-like growth factor transport and uptake. Furthermore, in C57BL/6 mice subjected to experimental wounds treated with 0.25% 2,4-DS, the histological parameters demonstrated a great capacity for vascular recovery. Additionally, this study confirmed that DS is a potent inducer of wound-healing cellular pathways and a promoter of neovascularization, being a natural ally in the tissue regeneration strategy. |
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| معلومات مُعتمدة: | E29/010.002843/2014 Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro |
| فهرسة مساهمة: | Keywords: Styela plicata; ascidian; dermatan sulfate; fibroblast; wound healing |
| المشرفين على المادة: | 24967-94-0 (Dermatan Sulfate) |
| تواريخ الأحداث: | Date Created: 20221110 Date Completed: 20221114 Latest Revision: 20221128 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9693086 |
| DOI: | 10.3390/md20110676 |
| PMID: | 36354999 |
| قاعدة البيانات: | MEDLINE |
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