دورية أكاديمية
Anti- Candida albicans Activity of Ononin and Other Secondary Metabolites from Platonia Insignis MART.
| العنوان: | Anti- Candida albicans Activity of Ononin and Other Secondary Metabolites from Platonia Insignis MART. |
|---|---|
| المؤلفون: | Silva AFD; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Biotechnology-RENORBIO, Federal University of Maranhão, São Luís 65080-805, Brazil., Farias JR; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Health Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil., Franco DCG; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Health Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil., Galiza AA; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Biotechnology-RENORBIO, Federal University of Maranhão, São Luís 65080-805, Brazil., Motta EP; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Health Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil., Oliveira ADS; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Health Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil., Vasconcelos CC; Program in Biotechnology-RENORBIO, Federal University of Maranhão, São Luís 65080-805, Brazil., Cartágenes MDSS; Program in Health Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil.; Laboratory of Experimental Study of Pain, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil., Rocha CQD; Department of Chemistry, Federal University of Maranhão, São Luís 65080-805, Brazil., Silva MCPD; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Health Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil., Lopes AJO; Federal Institute of Science Education and Technology of Maranhão-Campus Santa Inês, Santa Inês 65300-000, Brazil., Nascimento FRFD; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Health Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil., Monteiro CA; Department of Biology, Federal Institute of Science Education and Technology of Maranhão, São Luís 65030-005, Brazil., Guerra RNM; Laboratory of Immunophysiolgy, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Biotechnology-RENORBIO, Federal University of Maranhão, São Luís 65080-805, Brazil.; Program in Health Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil. |
| المصدر: | Metabolites [Metabolites] 2022 Oct 24; Vol. 12 (11). Date of Electronic Publication: 2022 Oct 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101578790 Publication Model: Electronic Cited Medium: Print ISSN: 2218-1989 (Print) Linking ISSN: 22181989 NLM ISO Abbreviation: Metabolites Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel : MDPI |
| مستخلص: | Candida albicans is a human pathogen that is part of the healthy microbiome. However, it is often associated with opportunistic fungal infections. The treatment of these infections is challenging because prolonged exposure to antifungal drugs can culminate in fungal resistance during therapy, and there is a limited number of available drugs. Therefore, this study investigated the antifungal activity of ononin by in silico and in vitro assays, and in Tenebrio molitor as an alternative in vivo model of infection caused by C. albicans . Ononin is an isoflavone glycoside derived from formononetin that has various biological activities. According in silico evaluation, ononin showed the best electron affinity in molecular docking with CaCYP51, with a binding free energy of -10.89 kcal/mol, superior to that of the antifungal drugs fluconazole and posaconazole. The ononin + CaCYP51 complex formed hydrogen bonds with Tyr132, Ser378, Phe380, and Met508, as well as hydrophobic connections with Tyr118, Leu121, Phe126, Leu131, Ile304, and Leu309, and interactions with the heme group. Ononin exerted anti- Candida albicans activity, with MIC between 3.9 and 7.8 µg/mL, and inhibited young and mature biofilms, with a reduction in cell density and metabolic activity of 50 to 80%. The compound was not cytotoxic to sheep red blood cells at concentrations up to 1000 µg/mL. Larvae of the mealworm T. molitor were used as an alternative in vivo model of C. albicans infection. Ononin was able to prolong larval survival at concentrations of 0.5, 1, and 5 mg/kg, and was not toxic up to a concentration of 20 mg/kg. Moreover, ononin reduced the fungal charge in treated animals. In conclusion, our results suggest that ononin has anti- Candida albicans activity and is a potential candidate for the development of new therapeutic alternatives. |
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| معلومات مُعتمدة: | PAEDT-03230/17 Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão |
| فهرسة مساهمة: | Keywords: Candida albicans; Platonia insignis; Tenebrio molitor; antifungal; medicinal chemistry; molecular docking; new drugs; ononin |
| تواريخ الأحداث: | Date Created: 20221110 Latest Revision: 20221129 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC9696916 |
| DOI: | 10.3390/metabo12111014 |
| PMID: | 36355097 |
| قاعدة البيانات: | MEDLINE |
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