دورية أكاديمية
أعرض في EDS

Avirulent phenotype promotes Bordetella pertussis adaptation to the intramacrophage environment.

التفاصيل البيبلوغرافية
العنوان: Avirulent phenotype promotes Bordetella pertussis adaptation to the intramacrophage environment.
المؤلفون: Farman MR; Institute for Theoretical Chemistry, University of Vienna, Vienna, Austria., Petráčková D; Czech Academy of Sciences, Laboratory of Post-transcriptional Control of Gene Expression, Institute of Microbiology, Prague, Czech Republic., Kumar D; Czech Academy of Sciences, Laboratory of Post-transcriptional Control of Gene Expression, Institute of Microbiology, Prague, Czech Republic., Držmíšek J; Czech Academy of Sciences, Laboratory of Post-transcriptional Control of Gene Expression, Institute of Microbiology, Prague, Czech Republic., Saha A; Czech Academy of Sciences, Laboratory of Post-transcriptional Control of Gene Expression, Institute of Microbiology, Prague, Czech Republic., Čurnová I; Czech Academy of Sciences, Laboratory of Post-transcriptional Control of Gene Expression, Institute of Microbiology, Prague, Czech Republic., Čapek J; Czech Academy of Sciences, Laboratory of Post-transcriptional Control of Gene Expression, Institute of Microbiology, Prague, Czech Republic., Hejnarová V; Czech Academy of Sciences, Laboratory of Post-transcriptional Control of Gene Expression, Institute of Microbiology, Prague, Czech Republic., Amman F; Institute for Theoretical Chemistry, University of Vienna, Vienna, Austria., Hofacker I; Institute for Theoretical Chemistry, University of Vienna, Vienna, Austria., Večerek B; Czech Academy of Sciences, Laboratory of Post-transcriptional Control of Gene Expression, Institute of Microbiology, Prague, Czech Republic.
المصدر: Emerging microbes & infections [Emerg Microbes Infect] 2023 Dec; Vol. 12 (1), pp. e2146536.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101594885 Publication Model: Print Cited Medium: Internet ISSN: 2222-1751 (Electronic) Linking ISSN: 22221751 NLM ISO Abbreviation: Emerg Microbes Infect Subsets: MEDLINE
أسماء مطبوعة: Publication: 2019- : [Philadelphia, PA] : Taylor & Francis
Original Publication: New York : NPG, 2012-2018.
مواضيع طبية MeSH: Bordetella pertussis*/metabolism , Whooping Cough*, Humans ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Phenotype ; Macrophages/metabolism ; Gene Expression Regulation, Bacterial
مستخلص: Bordetella pertussis , the causative agent of whooping cough, is an extracellular, strictly human pathogen. However, it has been shown that B. pertussis cells can escape phagocytic killing and survive in macrophages upon internalization. Our time-resolved RNA-seq data suggest that B. pertussis efficiently adapts to the intramacrophage environment and responds to host bactericidal activities. We show that this adaptive response is multifaceted and, surprisingly, related to the BvgAS two-component system, a master regulator of virulence. Our results show that the expression of this regulatory circuit is downregulated upon internalization. Moreover, we demonstrate that the switch to the avirulent Bvg - phase augments a very complex process based on the adjustment of central and energy metabolism, cell wall reinforcement, maintenance of appropriate redox and metal homeostasis, and repair of damaged macromolecules. Nevertheless, not all observed effects could be simply attributed to the transition to Bvg - phase, suggesting that additional regulators are involved in the adaptation to the intramacrophage environment. Interestingly, a large number of genes required for the metabolism of sulphur were strongly modulated within macrophages. In particular, the mutant lacking two genes encoding cysteine dioxygenases displayed strongly attenuated cytotoxicity toward THP-1 cells. Collectively, our results suggest that intracellular B. pertussis cells have adopted the Bvg - mode to acclimate to the intramacrophage environment and respond to antimicrobial activities elicited by THP-1 cells. Therefore, we hypothesize that the avirulent phase represents an authentic phenotype of internalized B. pertussis cells.
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فهرسة مساهمة: Keywords: Bordetella pertussis; BvgAS; adaptation to stress; avirulent phase; cysteine toxicity; intramacrophage environment
المشرفين على المادة: 0 (Bacterial Proteins)
تواريخ الأحداث: Date Created: 20221110 Date Completed: 20230123 Latest Revision: 20230124
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9858536
DOI: 10.1080/22221751.2022.2146536
PMID: 36357372
قاعدة البيانات: MEDLINE
الوصف
تدمد:2222-1751
DOI:10.1080/22221751.2022.2146536