دورية أكاديمية
Functional antibodies against G-protein coupled receptors in Beagle dogs infected with two different strains of Trypanosoma cruzi .
| العنوان: | Functional antibodies against G-protein coupled receptors in Beagle dogs infected with two different strains of Trypanosoma cruzi . |
|---|---|
| المؤلفون: | Wallukat G; Experimental and Clinical Research Center, Max-Delbrück Center for Molecular Medicine, Berlin, Germany.; Berlin Cures GmbH, Berlin, Germany., Botoni FA; Postgraduate Program in Infectiology and Tropical Medicine, School of Medicine, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.; Fundação Hospitalar do Estado de Minas Gerais, Belo Horizonte, MG, Brazil., Rocha MODC; Postgraduate Program in Infectiology and Tropical Medicine, School of Medicine, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil., Louise V; Postgraduate Program in Infectiology and Tropical Medicine, School of Medicine, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.; Department of Biological Sciences, Institute of Exact and Biological Sciences & Postgraduate Program of Health and Nutrition, School of Nutrition, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Müller J; Berlin Cures GmbH, Berlin, Germany., Talvani A; Postgraduate Program in Infectiology and Tropical Medicine, School of Medicine, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.; Department of Biological Sciences, Institute of Exact and Biological Sciences & Postgraduate Program of Health and Nutrition, School of Nutrition, Federal University of Ouro Preto, Ouro Preto, MG, Brazil. |
| المصدر: | Frontiers in immunology [Front Immunol] 2022 Oct 25; Vol. 13, pp. 926682. Date of Electronic Publication: 2022 Oct 25 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Chagas Cardiomyopathy* , Chagas Disease* , Trypanosoma cruzi*, Animals ; Dogs ; Rats ; Autoantibodies ; Receptor, Muscarinic M2 |
| مستخلص: | The interaction of the anti-beta1-adrenergic receptor autoantibodies (β1ARAb) and the anti-muscarinic M2 receptor autoantibodies (M2RAb) with cardiac neurotransmitter receptors were identified in human chronic Chagas cardiomyopathy (CCC) related to the ECG and dysautonomia disturbances. Dogs are considered gold model to the study of Trypanosoma cruzi infection due the clinical similarities with CCC. This study aims to evaluate whether anti-β1ARAb, anti-β2ARAb, and anti-muscarinic M2RAb are generated in Beagle dogs infected by T. cruzi using Y and Berenice-78 strains of T. cruzi. Animals were infected with 4.0 x 10 3 bloodstream trypomastigotes/kg of body weight and, after 25 months of infection, blood sample was collected, and serum stored at -80°C. Dog serum was treated by ammonium sulphate precipitation and the IgG antibodies isolated and added to the beating neonatal rats' cardiomyocytes. All T. cruzi -infected dogs developed agonistic β1ARAb, β2ARAb, and M2RAb. Animals infected by Berenice strain presented less β2ARAb and M2RAb activities than dogs infected by Y strain of the parasite. In cardiomyocytes culture, the antibodies recognized an epitope on the second extracellular loop of the receptors which were similar to findings in human Chagas disease. There was no detection of antibody against G protein-coupled receptor in serum from uninfected dogs. In conclusion, both Y and Berenice-78 strains of T. cruzi induced dog antibodies, whose targets located in the second extracellular loop of the adrenergic and muscarinic receptors were similar to those observed in individuals with CCC. Therefore, our findings highlight dogs as a promisor model to investigate pathogenic roles of functional Ab against G-protein coupled receptors. Competing Interests: Authors GW and JM were employed by company Berlin Cures GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Wallukat, Botoni, Rocha, Louise, Müller and Talvani.) |
| References: | Clin Exp Immunol. 1988 Dec;74(3):349-54. (PMID: 2853016) Front Immunol. 2018 Aug 06;9:1842. (PMID: 30127792) Circulation. 2007 Mar 6;115(9):1101-8. (PMID: 17339568) Eur J Heart Fail. 2012 Dec;14(12):1374-88. (PMID: 22892122) Mem Inst Oswaldo Cruz. 2022 Jun 06;117:e210172. (PMID: 35674528) Cardiovasc Res. 2003 Oct 15;60(1):96-107. (PMID: 14522411) PLoS One. 2019 Jul 5;14(7):e0214263. (PMID: 31276517) BMJ. 2021 Apr 9;373:n901. (PMID: 33837041) Front Microbiol. 2019 Aug 21;10:1856. (PMID: 31496999) Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3541-5. (PMID: 7536937) Vet Immunol Immunopathol. 2009 Jul 15;130(1-2):43-52. (PMID: 19211152) Mem Inst Oswaldo Cruz. 1992 Jan-Mar;87(1):59-71. (PMID: 1308556) FASEB J. 1997 Jan;11(1):77-83. (PMID: 9034169) J Clin Invest. 2004 May;113(10):1419-29. (PMID: 15146239) Mem Inst Oswaldo Cruz. 2008 Sep;103(6):528-34. (PMID: 18949320) Methods Mol Biol. 2019;1955:247-261. (PMID: 30868533) Int J Cardiol. 2016 Sep 15;219:98-104. (PMID: 27332740) PLoS Negl Trop Dis. 2014 Dec 18;8(12):e3384. (PMID: 25521296) Int J Cardiol. 2010 Nov 19;145(2):339-340. (PMID: 20051298) J Mol Cell Cardiol. 1995 Jan;27(1):397-406. (PMID: 7539084) Bol Oficina Sanit Panam. 1961 Nov;51:404-28. (PMID: 14457497) J Infect Dis. 2008 Feb 15;197(4):544-7. (PMID: 18237267) Circulation. 2000 Feb 1;101(4):385-91. (PMID: 10653829) Nat Rev Cardiol. 2012 Oct;9(10):576-89. (PMID: 22847166) J Am Coll Cardiol. 2010 Feb 2;55(5):463-8. (PMID: 20117461) Biomed Biochim Acta. 1987;46(8-9):S634-9. (PMID: 2449194) Mem Inst Oswaldo Cruz. 1999;94 Suppl 1:257-62. (PMID: 10677729) Microbes Infect. 2006 Aug;8(9-10):2459-64. (PMID: 16963301) Heart Fail Rev. 2012 Jan;17(1):45-64. (PMID: 21165698) Int J Cardiol. 2014 Jan 1;170(3):e66-7. (PMID: 24268984) Arch Pathol Lab Med. 1981 Sep;105(9):460-4. (PMID: 6791609) Acta Trop. 2014 Nov;139:57-66. (PMID: 25017312) J Mol Cell Cardiol. 1995 Jan;27(1):427-36. (PMID: 7539085) Clin Exp Immunol. 1984 Sep;57(3):679-86. (PMID: 6088139) |
| فهرسة مساهمة: | Keywords: Cardiomyocytes; Trypanosoma cruzi; autoantibodies; chagas disease; dog model; g-protein coupled receptors |
| المشرفين على المادة: | 0 (Autoantibodies) 0 (Receptor, Muscarinic M2) |
| تواريخ الأحداث: | Date Created: 20221117 Date Completed: 20221122 Latest Revision: 20221222 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9641151 |
| DOI: | 10.3389/fimmu.2022.926682 |
| PMID: | 36389686 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
|---|---|
| DOI: | 10.3389/fimmu.2022.926682 |