دورية أكاديمية
Differential phosphorylation of Clr4 SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis.
| العنوان: | Differential phosphorylation of Clr4 SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis. |
|---|---|
| المؤلفون: | Kuzdere T; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.; University of Basel, Basel, Switzerland., Flury V; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.; University of Basel, Basel, Switzerland., Schalch T; Department of Molecular and Cell Biology, Leicester Institute of Structural and Chemical Biology, University of Leicester, Leicester, UK., Iesmantavicius V; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland., Hess D; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland., Bühler M; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.; University of Basel, Basel, Switzerland. |
| المصدر: | EMBO reports [EMBO Rep] 2023 Jan 09; Vol. 24 (1), pp. e55928. Date of Electronic Publication: 2022 Nov 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 100963049 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-3178 (Electronic) Linking ISSN: 1469221X NLM ISO Abbreviation: EMBO Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2024- : [London] : Nature Publishing Group Original Publication: Oxford, UK : Published for EMBO by Oxford University Press, 2000- |
| مواضيع طبية MeSH: | Schizosaccharomyces pombe Proteins*/genetics , Schizosaccharomyces pombe Proteins*/metabolism , Schizosaccharomyces*/genetics , Schizosaccharomyces*/metabolism, Methylation ; Histones/genetics ; Histones/metabolism ; Phosphorylation ; Heterochromatin/metabolism ; Histone-Lysine N-Methyltransferase/genetics ; Histone-Lysine N-Methyltransferase/metabolism ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Methyltransferases/genetics ; Methyltransferases/metabolism ; Gametogenesis/genetics |
| مستخلص: | Methylation of histone H3 at lysine 9 (H3K9) is a hallmark of heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- and tri-methylation of H3K9. Although H3K9 methylation has been intensely studied in mitotic cells, its role during sexual differentiation remains unclear. Here, we map H3K9 methylation genome-wide during meiosis and show that constitutive heterochromatin temporarily loses H3K9me2 and becomes H3K9me3 when cells commit to meiosis. Cells lacking the ability to tri-methylate H3K9 exhibit meiotic chromosome segregation defects. Finally, the H3K9 methylation switch is accompanied by differential phosphorylation of Clr4 by the cyclin-dependent kinase Cdk1. Our results suggest that a conserved master regulator of the cell cycle controls the specificity of an H3K9 methyltransferase to prevent ectopic H3K9 methylation and to ensure faithful gametogenesis. (© 2022 Friedrich Miescher Institute for Biomedical Research. Published under the terms of the CC BY 4.0 license.) |
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| معلومات مُعتمدة: | BB/S018549 United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
| فهرسة مساهمة: | Keywords: chromosome segregation; fission yeast; histone methylation; meiosis; phosphorylation |
| المشرفين على المادة: | 0 (Histones) 0 (Schizosaccharomyces pombe Proteins) 0 (Heterochromatin) EC 2.1.1.43 (Histone-Lysine N-Methyltransferase) 0 (Cell Cycle Proteins) EC 2.1.1.- (Methyltransferases) EC 2.1.1.43 (clr4 protein, S pombe) |
| تواريخ الأحداث: | Date Created: 20221121 Date Completed: 20230110 Latest Revision: 20230114 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9827552 |
| DOI: | 10.15252/embr.202255928 |
| PMID: | 36408846 |
| قاعدة البيانات: | MEDLINE |
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