دورية أكاديمية
أعرض في EDS

The association of hyperketonemia with fecal and rumen microbiota at time of diagnosis in a case-control cohort of early lactation cows.

التفاصيل البيبلوغرافية
العنوان: The association of hyperketonemia with fecal and rumen microbiota at time of diagnosis in a case-control cohort of early lactation cows.
المؤلفون: Miles AM; Department of Animal Science, College of Agricultural Sciences, The Pennsylvania State University, University Park, State College, PA, 16802, USA.; Current address: Animal Genomics and Improvement Laboratory, Agricultural Research Service, United States Department of Agriculture (USDA), Beltsville, MD, 20705, USA., McArt JAA; Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA., Lima SF; Department of Medicine, Jill Roberts Institute for IBD Research, Weill Cornell Medicine, New York, NY, 10021, USA., Neves RC; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, Lafayette, IN, 47907, USA., Ganda E; Department of Animal Science, College of Agricultural Sciences, The Pennsylvania State University, University Park, State College, PA, 16802, USA. ganda@psu.edu.
المصدر: BMC veterinary research [BMC Vet Res] 2022 Nov 21; Vol. 18 (1), pp. 411. Date of Electronic Publication: 2022 Nov 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Cattle Diseases*/diagnosis , Ketosis*/veterinary , Microbiota*, Female ; Cattle ; Pregnancy ; Animals ; Rumen/metabolism ; Case-Control Studies ; RNA, Ribosomal, 16S/genetics ; Prospective Studies ; Milk/metabolism ; Lactation ; 3-Hydroxybutyric Acid
مستخلص: Background: Many dairy cows experience a state of energy deficit as they transition from late gestation to early lactation. The aims of this study were to 1) determine if the development of hyperketonemia in early lactation dairy cows is indicated by their gut microbiome, and 2) to identify microbial features which may inform health status. We conducted a prospective nested case-control study in which cows were enrolled 14 to 7 days before calving and followed through their first 14 days in milk (DIM). Hyperketonemic cows (HYK, n = 10) were classified based on a blood β-hydroxybutyrate (BHB) concentration 1.2 mmol/L within their first 14 DIM. For each HYK cow, two non-HYK (CON, n = 20) cows were matched by parity and 3 DIM, with BHB < 1.2 mmol/L. Daily blood BHB measures were used to confirm CON cows maintained their healthy status; some CON cows displayed BHB 1.2 mmol/L after matching and these cows were reclassified as control-HYK (C-HYK, n = 9). Rumen and fecal samples were collected on the day of diagnosis or matching and subjected to 16S rRNA profiling.
Results: No differences in taxa abundance, or alpha and beta diversity, were observed among CON, C-HYK, and HYK health groups for fecal microbiomes. Similar microbiome composition based on beta diversity analysis was detected for all health statuses, however the rumen microbiome of CON and HYK cows were found to be significantly different. Interestingly, highly similar microbiome composition was observed among C-HYK cow rumen and fecal microbiomes, suggesting that these individual animals which initially appear healthy with late onset of hyperketonemia were highly similar to each other. These C-HYK cows had significantly lower abundance of Ruminococcus 2 in their rumen microbiome compared to CON and HYK groups. Multinomial regressions used to compute log-fold changes in microbial abundance relative to health status were not found to have predictive value, therefore were not useful to identify the role of certain microbial features in predicting health status.
Conclusions: Lower relative abundance of Ruminococcus 2 in C-HYK cow rumens was observed, suggesting these cows may be less efficient at degrading cellulose although the mechanistic role of Ruminococcus spp. in rumen metabolism is not completely understood. Substantial differences in fecal or rumen microbiomes among cows experiencing different levels of energy deficit were not observed, suggesting that hyperketonemia may not be greatly influenced by gut microbial composition, and vice versa. Further studies using higher resolution -omics approaches like meta-transcriptomics or meta-proteomics are needed to decipher the exact mechanisms at play.
(© 2022. The Author(s).)
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معلومات مُعتمدة: This work is/was supported by the USDA National Institute of Food and Agriculture and Hatch Appropriations under Project #PEN04752 Accession #1023328. U.S. Department of Agriculture
فهرسة مساهمة: Keywords: 16S rRNA sequencing; Dairy cow; Gut microbiome; Hyperketonemia; Metagenomics
المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
TZP1275679 (3-Hydroxybutyric Acid)
تواريخ الأحداث: Date Created: 20221121 Date Completed: 20221123 Latest Revision: 20221213
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9677665
DOI: 10.1186/s12917-022-03500-4
PMID: 36411435
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-022-03500-4