دورية أكاديمية
The endocytic recycling compartment serves as a viral factory for hepatitis E virus.
| العنوان: | The endocytic recycling compartment serves as a viral factory for hepatitis E virus. |
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| المؤلفون: | Bentaleb C; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Hervouet K; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Montpellier C; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Camuzet C; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Ferrié M; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Burlaud-Gaillard J; Inserm U1259, Morphogénèse et Antigénicité du VIH et des Virus des Hépatites (MAVIVH), Université de Tours and CHRU de Tours, 37032, Tours, France.; Université de Tours et CHRU de Tours, Plateforme IBiSA de Microscopie Electronique, Tours, France., Bressanelli S; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-Sur-Yvette, France., Metzger K; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Werkmeister E; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, UMR2014-US41-PLBS-Plateformes Lilloises de Biologie and Santé, Lille, France., Ankavay M; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France.; Division of Gastroenterology and Hepatology, Institute of Microbiology, Lausanne, Switzerland., Janampa NL; Inserm U1259, Morphogénèse et Antigénicité du VIH et des Virus des Hépatites (MAVIVH), Université de Tours and CHRU de Tours, 37032, Tours, France., Marlet J; Inserm U1259, Morphogénèse et Antigénicité du VIH et des Virus des Hépatites (MAVIVH), Université de Tours and CHRU de Tours, 37032, Tours, France., Roux J; BIOTEM, Apprieu, France., Deffaud C; BIOTEM, Apprieu, France., Goffard A; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Rouillé Y; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Dubuisson J; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Roingeard P; Inserm U1259, Morphogénèse et Antigénicité du VIH et des Virus des Hépatites (MAVIVH), Université de Tours and CHRU de Tours, 37032, Tours, France.; Université de Tours et CHRU de Tours, Plateforme IBiSA de Microscopie Electronique, Tours, France., Aliouat-Denis CM; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France., Cocquerel L; University of Lille, CNRS, Inserm, CHU Lille, Pasteur Institute of Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France. laurence.cocquerel@cnrs.fr. |
| المصدر: | Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2022 Dec 03; Vol. 79 (12), pp. 615. Date of Electronic Publication: 2022 Dec 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Switzerland NLM ID: 9705402 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-9071 (Electronic) Linking ISSN: 1420682X NLM ISO Abbreviation: Cell Mol Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Basel : Springer Original Publication: Basel ; Boston : Birkhauser, c1997- |
| مواضيع طبية MeSH: | Hepatitis E virus*/genetics, Humans ; Viral Replication Compartments ; Capsid Proteins ; Biological Transport ; Antibodies, Monoclonal |
| مستخلص: | Although hepatitis E virus (HEV) is the major leading cause of enterically transmitted viral hepatitis worldwide, many gaps remain in the understanding of the HEV lifecycle. Notably, viral factories induced by HEV have not been documented yet, and it is currently unknown whether HEV infection leads to cellular membrane modeling as many positive-strand RNA viruses. HEV genome encodes the ORF1 replicase, the ORF2 capsid protein and the ORF3 protein involved in virion egress. Previously, we demonstrated that HEV produces different ORF2 isoforms including the virion-associated ORF2i form. Here, we generated monoclonal antibodies that specifically recognize the ORF2i form and antibodies that recognize the different ORF2 isoforms. One antibody, named P1H1 and targeting the ORF2i N-terminus, recognized delipidated HEV particles from cell culture and patient sera. Importantly, AlphaFold2 modeling demonstrated that the P1H1 epitope is exposed on HEV particles. Next, antibodies were used to probe viral factories in HEV-producing/infected cells. By confocal microscopy, we identified subcellular nugget-like structures enriched in ORF1, ORF2 and ORF3 proteins and viral RNA. Electron microscopy analyses revealed an unprecedented HEV-induced membrane network containing tubular and vesicular structures. We showed that these structures are dependent on ORF2i capsid protein assembly and ORF3 expression. An extensive colocalization study of viral proteins with subcellular markers, and silencing experiments demonstrated that these structures are derived from the endocytic recycling compartment (ERC) for which Rab11 is a central player. Hence, HEV hijacks the ERC and forms a membrane network of vesicular and tubular structures that might be the hallmark of HEV infection. (© 2022. The Author(s).) |
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| معلومات مُعتمدة: | MAT-PI-17006 Inserm Transfert; DiagHepE Institut Pasteur de Lille; DiagHepE Région Hauts-de-France |
| فهرسة مساهمة: | Keywords: AlphaFold2; Antibodies; Electron microscopy; Endocytic recycling compartment; Hepatitis E virus; Infectious particles; ORF2 capsid protein; Rab11; Viral factories |
| المشرفين على المادة: | 0 (Capsid Proteins) 0 (Antibodies, Monoclonal) |
| تواريخ الأحداث: | Date Created: 20221202 Date Completed: 20221206 Latest Revision: 20221213 |
| رمز التحديث: | 20221214 |
| مُعرف محوري في PubMed: | PMC9718719 |
| DOI: | 10.1007/s00018-022-04646-y |
| PMID: | 36460928 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1420-9071 |
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| DOI: | 10.1007/s00018-022-04646-y |